Patch-clamp amplifiers from single channels to large macroscopic recordings
The Axon Instruments® series of amplifiers provide best-in-class solutions for the entire range of patch-clamp experiments. The portfolio of amplifiers includes Axopatch™ 200B for ultra low-noise single-channel recordings, MultiClamp™ 700B for whole-cell voltage-clamp and high-speed current-clamp recordings, and Axoclamp™ 900A for two-electrode voltage-clamp and current-clamp recordings.
Minimize signal to noise ratio
The Axopatch 200B Capacitor Feedback Patch Clamp Amplifier offers one of the lowest-noise single-channel recordings available via innovative capacitor-feedback technology.
Perform multi-channel experiments
The MultiClamp 700B Microelectrode Amplifier enables whole-cell voltage-clamp and current-clamp recordings. It is the most versatile amplifier in the portfolio.
Measure large currents
Large output compliance range of our Axoclamp 900A Microelectrode Amplifier facilitates the measurement of large and rapid voltage-clamp and current-clamp recordings.
Actively cooled headstage
The Axopatch 200B amplifier features proprietary technology that provides active headstage cooling that reduces electrical noise close to the theoretical limits of physics.
Software control of settings
The MultiClamp 700B and Axoclamp 900A amplifiers offer software control. Software control streamlines setup, and enables automation of parameters, telegraphing, and advanced protocols.
Support up to four headstages
The MultiClamp 700B supports up to two primary CV-7B headstages and two optional auxiliary headstages (HS-2 or VG-2 type) enabling multi-channel recording for cellular network studies.
Large output compliance range
The Axoclamp 900A amplifier supports the measurement of larger currents and ensures faster clamp speed (±180 V in TEVC and HVIC modes).
Multiple modes of operation
The Axoclamp 900A amplifier offers 5 modes of operation: current clamp, discontinuous current clamp, two-electrode voltage clamp, discontinuous single-electrode voltage clamp, high-voltage current clamp.
Works with any data acquisition system
The family of amplifiers integrates with most data acquisition programs. The pCLAMP™ 11 Software and DigiData® 1550B system for data acquisition and analysis provide optimal performance.
Which amplifier is right for me?
|Axopatch 200B Amplifier||MultiClamp 700B Amplifier||Axoclamp 900A Amplifier|
|Extracellular field-potential recording||
|Intracellular sharp-electrode recording||
|Two-electrode voltage-clamp recording||
Specifications & Options of Axon Instruments Patch-Clamp Amplifiers
* Holding level, current passing, filter option, multiple signal outputs, pipette offset, fast and whole cell capacitance compensation, series compensation, pipette neutralization, bridge balance
Resources of Axon Instruments Patch-Clamp Amplifiers
Explore this Axon Axoclamp 900A Microelectrode Amplifier datasheet. this instrument offers several modes of operation that measure signals from single cells, tissue slices and whole…
Download datasheet to learn about Axon Axopatch 200B Microelectrode Amplifier which includes cooling of the active elements to achieve the lowest possible electrical noise.
NMDAR ion channels are found in neurons and are frequent targets of research efforts. In addition to potentially playing a key role in learning and memory, it is also a target for re…
By the end of the tutorial, you will be ready to acquire data and understand how to tailor setup configurations to meet your needs. We will explain standard setup for configuring the…
A complete workflow solution for patch-clamp electrophysiology
For the latest featured videos, webinars and tutorials on our Axon instrument solution including Axon Patch-clamp Amplifiers, Digidata 1550B Digitizer plus HumSilencer, and pCLAMP Software Suite, visit our Axon Patch-Clamp Video Gallery.
Tech Tips with Jeffrey Tang: An introduction to the Humsilencer
How to Combine Traces, Calculate Rise or Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
Using Electrophysiological Studies to Accelerate Mechanistic Study in Reception and Transmission
Update and Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning
Investigations of the Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in a Xenopus Oocyte Model
Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies
- Dated: Nov 30, 2020Publication Name: Biophysical Journal
Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA MoleculesSingle molecules of DNA or RNA can be detected as they are driven through an α-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential, single-file order because the limiting diameter of the pore can accommodate only one strand of DNA or RNA at a… View more
Single molecules of DNA or RNA can be detected as they are driven through an α-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential, single-file order because the limiting diameter of the pore can accommodate only one strand of DNA or RNA at a time. Here we demonstrate that this nanopore behaves as a detector that can rapidly discriminate between pyrimidine and purine segments along an RNA molecule. Nanopore detection and characterization of single molecules represent a new method for directly reading information encoded in linear polymers, and are critical first steps toward direct sequencing of individual DNA and RNA molecules.Contributors: Mark Akeson, Daniel Branton, John J.Kasianowicz § EricBrandin, David W.Deamer
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- Dated: Mar 29, 2006Publication Name: Journal of Neuroscience
Persistent Sodium Current in Layer 5 Neocortical Neurons Is Primarily Generated in the Proximal AxonIn addition to the well described fast-inactivating component of the Na+ current [transient Na+ current (INaT)], neocortical neurons also exhibit a low-voltage-activated, slowly inactivating “persistent” Na+ current (INaP), which plays a role in determining neuronal excitability and synaptic integration. We investigated the Na+ channels… View more
In addition to the well described fast-inactivating component of the Na+ current [transient Na+ current (INaT)], neocortical neurons also exhibit a low-voltage-activated, slowly inactivating “persistent” Na+ current (INaP), which plays a role in determining neuronal excitability and synaptic integration. We investigated the Na+ channels responsible for INaP in layer 5 pyramidal cells using cell-attached and whole-cell recordings in neocortical slices. In simultaneous cell-attached and whole-cell somatic recordings, no persistent Na+ channel activity was detected at potentials at which whole-cell INaP operates. Detailed kinetic analysis of late Na+ channel activity in cell-attached patches at 36°C revealed that somatic Na+ channels do not demonstrate “modal gating” behavior and that the probability of single late openings is extremely low (<1.4 × 10−4 or <0.02% of maximal open probability of INaT). Ensemble averages of these currents did not reveal a sustained component whose amplitude and voltage dependence could account for INaP as seen in whole-cell recordings.Contributors: Nadav Astman, Michael J. Gutnick and Ilya A. Fleidervish
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- Dated: Sep 15, 1996Publication Name: American Chemical SocietyMany of the molecules involved in biological signaling processes are easily oxidized and have been monitored by electrochemical methods. Temporal response, spatial considerations, and sensitivity of the electrodes must be optimized for the specific biological application. To monitor exocytosis from single cells in culture, constant potential… View more
Many of the molecules involved in biological signaling processes are easily oxidized and have been monitored by electrochemical methods. Temporal response, spatial considerations, and sensitivity of the electrodes must be optimized for the specific biological application. To monitor exocytosis from single cells in culture, constant potential amperometry offers the best temporal resolution, and a low-noise picoammeter improves the detection limits. Smaller electrodes, with 1-μm diameters, provided spatial resolution sufficient to identify the locations of release sites on the surface of single cells. For the study of neurotransmitter release in vivo, larger cylindrical microelectrodes are advantageous because the secreted molecules come from multiple terminals near the electrode, and the greater amounts lead to a larger signal that emerges from the Johnson noise of the current amplifier. With this approach, dopamine release elicited by two electrical stimulus pulses at 10 Hz was detected with fast-scan cyclic voltammetry in vivo. Nafion-coated elliptical electrodes have previously been shown to be incapable of detecting such concentration changes without extensive signal averaging. In addition, we demonstrate that high-pass filtering (200 Hz) of cyclic voltammograms recorded at 300 V/s decreases the background current and digitization noise at these microelectrodes, leading to an improved signal. Also, high-pass filtering discriminated against ascorbic acid, DOPAC, and acidic pH changes, three common interferences in vivo.Contributors: Paula S. Cahill, Q. David Walker, Jennifer M. Finnegan, George E. Mickelson, Eric R. Travis, and R. Mark Wightman
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Axon Instruments Patch-Clamp Amplifiers
|Axoclamp 900A Headstage HS-9A X0.1U||x0.1 headstage||1-2950-0359|
|Axoclamp 900A Headstage HS-9A X1U||x1 headstage||1-2950-0360|
|Axoclamp 900A Headstage HS-9A X10U||x10 headstage||1-2950-0361|
|Axoclamp 900A Headstage VG-9A X10U||x10 virtual ground headstage||1-2950-0362|
|Axoclamp 900A Headstage VG-9A X100U||x100 virtual ground headstage||1-2950-0363|
|MultiClamp 700B Headstage CV-7B||Patch-clamp headstage||1-CV-7B|
|MultiClamp 700B Headstage CV-7B/BL||Bilayer headstage||1-CV-7B/BL|
|MultiClamp 700B Headstage CV-7B/EC||Electrochemistry headstage||1-CV-7B/EC|
Electrode holders, adapters, and holder components
|Electrode Holder for U-Type Headstages||Fits glass pipettes with outer diameter of 1.0 - 1.7 mm||1-HL-U|
|Electrode Holder Replacement Caps||Set of 2 polycarbonate caps for HL-U holders||1-HL-CAP|
|Cone Washers 1.1mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0 - 1.1 mm||1-HLC-11|
|Cone Washers 1.3mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1 - 1.3 mm||1-HLC-13|
|Cone Washers 1.5mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3 - 1.5 mm||1-HLC-15|
|Cone Washers 1.7mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5 - 1.7 mm||1-HLC-17|
|Pins 1mm for HL-U Holders||Set of 3 brass pins for HL-U holders, 1mm||1-HLP-U|
|2mm Plugs with Solder Cups||Set of 5 general purpose gold plugs, 2mm, with solder cups||1-HLP-0|
|Silver Wire||Set of 5 Ag wires, 0.25mm diameter, 50mm long||1-HLA-005|
|Silicone Tubing for Silver Wire||1mm ID x 70mm Long Silicone Tubing||1-HLT-70|
|Silver/Silver Chloride Pellet Assemblies||Set of 3 Ag/AgCl pellet assemblies||1-HLA-003|
|Adapter For BNC Holders To U-Type Headstages||Connects BNC holders to CV and HS headstages with threaded collets (U-type)||1-HLB-U|
|Right-Angle Adapter for HL-U Electrode Holders||Fits CV and HS headstages with threaded collets (U-type)||1-HLR-U|
|Model Cell for Oocytes||Axoclamp/ GeneClamp model cell for oocytes. Connects to U-type HS series headstages||1-MCO-2U|
|Model Cell for TEVC / DSEVC||Axoclamp/ GeneClamp model cell for two-electrode voltage clamp/ discontinuous single-electrode voltage-clamp conditions. Connects to U-type HS series headstages||1-CLAMP-1U|
|Model Cell for Whole Cell/ Single Channels||Axopatch/ GeneClamp/ MultiClamp model cell for whole-cell / single-channel patch-clamp conditions. Connects to U-type CV series headstages||1-PATCH-1U|
|Model Cell for Bilayers||Axopatch/ GeneClamp/ MultiClamp model cell for bilayer conditions. Connects to U-type CV series headstages||1-MCB-1U|
|Cable To Connect Axoclamp 2 Headstages To Axoclamp 900A Amplifier||Allows Axoclamp 2 headstages (HS-2, VG-2) to be used on Axoclamp 900A Amplifiers||1-2100-0934|
|SoftPanel Amplifier Control Unit||Provides physical knob and button control for computer-controlled Axoclamp 900-series and MultiClamp 700-series amplifiers. Requires a USB connection.||1-SOFTPANEL (USB)|
|Remote Buzz For Axoclamp 900A||Hand-held buzz duration control for Axoclamp 900A Amplifier (1-50ms)||1-2950-0366|
|Silver/Silver Chloride Pellet Assemblies||Set of 3 Ag/AgCl pellet assembly and Ag wire||1-HLA-003|
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