Electrophysiology and Biophysics Laboratory Techniques

The Axon Guide Fifth Edition

What you will find in this guide

This guide serves as an information and data resource for electrophysiologists. It covers a broad range of topics ranging from the biological basis of bioelectricity and a description of the basic experimental setup to a discussion of mechanisms of noise and data analysis.

The Axon Guide Fifth Edition is a tool benefiting both the novice and the expert electrophysiologist. Newcomers to electrophysiology will gain an appreciation of the intricacies of electrophysiological measurements and the requirements for setting up a complete recording and analysis system. For experienced electrophysiologists, we include in-depth discussions of selected topics, such as advanced methods in electrophysiology and noise.

For the fifth edition, we are very pleased to be able to offer new content in the Advanced Methods in Electrophysiology section, including advanced methods developed for ion channel recording from liposomes, planar bilayers, and bacterial spheroplasts; current recording from solid-state nanopores, and the use of nanopipettes in intracellular recording.

Table of contents

[Click to view sample]

Electric Circuit
Glass Microelectrodes
Aperture Formation in Plastic cup
Pre-Treatment of Muscle Cells


The most comprehensive laboratory techniques workbook—with over 250 pages—to support electrophysical and biophysical research

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Learn more about the Axon Instruments® portfolio:

Axon Instruments Patch-Clamp Amplifiers

Patch-clamp amplifiers from single channel, whole-cell, and two electrode recordings

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Axon Instruments Patch-Clamp Amplifiers

The Axon™ Digidata® 1550B plus HumSilencer®

High-resolution, low-noise digitizer with 50/60 Hz line-frequency noise elimination

Axon™ Digidata® 1550B plus HumSilencer®
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  • The HumSilencer option with the Digidata range eliminates of 50 and 60Hz line-frequency noise, making the biological signal clearer without distorting the data

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pCLAMP 11 Software Suite

A complete workflow solution for patch-clamp electrophysiology

  • Enhanced protocol editor in pCLAMP 11 Software allows for longer and more sophisticated protocols to be programmed and reduces protocol setup time
  • Multiple windows can be simultaneously displayed during a Membrane Test protocol, making it easier to patch multiple cells
  • Gap-free mode allows the programming of command and digital outputs in a continuous recording, allowing you to enhance protocol design and trigger actions within the protocol automatically

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pCLAMP 11 Software Suite

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