The digitizer that enables acquisition of patch-clamp data

The Axon™ Digidata® 1550B plus HumSilencer® is one of the most advanced analog-to-digital signal converters on the market, offering the innovative line synchronous noise-cancelling feature, HumSilencer. This advanced feature learns, adapts, and eliminates local 50/60 Hz line-frequency noise and high-frequency harmonics from incoming signals. The digitizer works with pCLAMPTM 11 Software for data acquisition and analysis. The software supports eight channels of analog acquisition and four acquisition modes.

  • Eliminate line-frequency noise

    Eliminate line-frequency noise

    HumSilencer removes 50/60 hz line-frequency noise on up to four channels in parallel. The function is quickly enabled through a software checkbox.

  • Record high-resolution traces

    Record high resolution traces

    All eight analog input channels can be simultaneously digitized at an impressive sampling rate of 500 kHz per channel.

  • Digitize cellular network studies

    Digitize cellular network studies

    With Digidata 1550B system, you can now record cellular networks without 50/60 Hz line-frequency noise in a single digitizer while saving money, time, and reducing hassle.

Axon Digidata 1550B Low Noise Data Acquisition System

Introducing the Axon Digidata 1550B Low Noise Data Acquisition System plus HumSilencer

Features

  • 50/60 hz noise cancellation

    HumSilencer is an advanced feature that learns, adapts, and eliminates local 50/60 Hz line-frequency noise patterns and associated high-frequency harmonics from incoming signal up to 20 V peak-to-peak.

  • Noise canceling

    HumSilencer is not a filter and has no effect on acquired signals. It causes no signal distortion such as frequency change, amplitude attenuation, phase shift, or DC voltage change.

  • Wide range of input signals

    Digidata 1550B digitizes a wide range of input signals from -10 to +10 V.

  • HumSilencer adaptation

    HumSilencer automatically recognizes changes in noise amplitudes and adapts by removing noise in less than one second.

  • Optimized for pCLAMP 11 Software

    The pCLAMP Software suite is the most widely-used patch-clamp electrophysiology data acquisition and analysis program for control and recording of voltage-clamp, current-clamp, and patch-clamp experiments.

  • Three configurations

    Three configurations are available: Digidata 1550B4 with four HumSilencer channels, Digidata 1550B1 with one HumSilencer channel, and Digidata 1550B0 without a HumSilencer channel.

Applications of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

  • Patch-Clamp Electrophysiology Digital Acquisition

    Digital Acquisition

    The current or voltage signal acquired by the amplifier is an analog signal, but to perform data analysis needed for high resolution patch-clamp measurements, the analog signal must be converted into a digital one. Positioned between the amplifier and the computer, the digitizer accomplishes this important task. Signal quality is extremely important and is impacted by the sampling frequency. The latest generation of Digidata digitizers sample at 500 kHz and can be equipped with HumSilencer, which eliminates 50/60 Hz line-frequency noise.

  • HumSilencer Technology

    HumSilencer Technology

    50/60 Hz line-frequency noise, also known as electrical hum, is the most common source of background noise in patch-clamp electrophysiology experiments. This noise can overwhelm biological signals of interest, making sensitive patch-clamp measurements nearly impossible. Traditional trouble shooting is typically only partially effective and can impair data accuracy. HumSilencer is a filter-free, adaptive technology that learns and removes line frequency noise without using methods that impair signal accuracy like filters, that can distort biological signals.

  •  Cellular Pathway Analysis

    Cellular Pathway Analysis

    Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, and neuronal communications.

  • Ion Channels used in Disease Research

    Disease Research

    Ion channels play a role in many diseases including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain, and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease.

Specifications & Options of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Resources of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Videos and Demos

Axon Digidata 1550B Low Noise Data Acquisition System

Introducing the Axon Digidata 1550B Low Noise Data Acquisition System plus HumSilencer

Calculate Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software

How to Combine Traces, Calculate Rise or Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software

Create Customized Command Waveforms Using pCLAMP Software

How to Create Customized Command Waveforms Using the pCLAMP Software

Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software

The Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software

Synchronizing Electrophysiology and Imaging Solutions with pCLAMP and MetaMorph Software

Synchronizing Electrophysiology and Imaging Solution with Axon pCLAMP and MetaMorph Software

Membrane Test Between Sweeps in Clampex & Analysis of Synaptic Events with Clampfit Data Analysis

Online Statistics, Membrane Test Between Sweeps in Clampex and Analysis of Synaptic Events with the Clampfit™ Data Analysis

Use of Axoporator for Single-cell Electroporation for Transfection and Dye-labeling

Use of the Axoporator 800A for Single-cell Electroporation for Transfection and Dye-labeling

Using Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels

Using the Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels

Use of Filters in Data Acquisition and the Clampfit Application

Writing Long-Term Potentiation and Depression Protocols and the Use of Filters in Data Acquisition and the Clampfit Application

Resistance Compensated Series

Series Resistance Compensated or Not

Using Electrophysiological Studies to Accelerate Mechanistic in Reception and Transmission

Using Electrophysiological Studies to Accelerate Mechanistic Study in Reception and Transmission

Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning

Update and Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning

Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in Xenopus Oocyte Model

Investigations of the Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in a Xenopus Oocyte Model

Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies

Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies

Chinese Version - Axon Amplifiers and pCLAMP Software Key Features Reviews

Axon Amplifiers and pCLAMP Software-key Features Reviews (Chinese version)

Using Clampfit in Basic Single Channel Analysis

Basic Single Channel Analysis Using Clampfit

Action Potential Analysis in Clampfit Module

Action Potential Analysis in Clampfit Module

A Walkthrough of Protocol Editor in pCLAMP Data Acquisition Module

A Walkthrough of the Protocol Editor in the pCLAMP Data Acquisition Module

A Walkthrough of Protocol Editor in pCLAMP (Chinese version)

A Walkthrough of Protocol Editor in pCLAMP (Chinese version)

Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Headstages

Description Details Part Number
Axoclamp 900A Headstage HS-9A X0.1U x0.1 headstage 1-2950-0359
Axoclamp 900A Headstage HS-9A X1U x1 headstage 1-2950-0360
Axoclamp 900A Headstage HS-9A X10U x10 headstage 1-2950-0361
Axoclamp 900A Headstage VG-9A X10U x10 virtual ground headstage 1-2950-0362
Axoclamp 900A Headstage VG-9A X100U x100 virtual ground headstage 1-2950-0363
MultiClamp 700B Headstage CV-7B Patch-clamp headstage 1-CV-7B
MultiClamp 700B Headstage CV-7B/BL Bilayer headstage 1-CV-7B/BL
MultiClamp 700B Headstage CV-7B/EC Electrochemistry headstage 1-CV-7B/EC

Electrode holders, adapters, and holder components

Description Details Part Number
Electrode Holder for U-Type Headstages Fits glass pipettes with outer diameter of 1.0 - 1.7 mm 1-HL-U
Electrode Holder Replacement Caps Set of 2 polycarbonate caps for HL-U holders 1-HL-CAP
Cone Washers 1.1mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0 - 1.1 mm 1-HLC-11
Cone Washers 1.3mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1 - 1.3 mm 1-HLC-13
Cone Washers 1.5mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3 - 1.5 mm 1-HLC-15
Cone Washers 1.7mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5 - 1.7 mm 1-HLC-17
Pins 1mm for HL-U Holders Set of 3 brass pins for HL-U holders, 1mm 1-HLP-U
2mm Plugs with Solder Cups Set of 5 general purpose gold plugs, 2mm, with solder cups 1-HLP-0
Silver Wire Set of 5 Ag wires, 0.25mm diameter, 50mm long 1-HLA-005
Silicone Tubing for Silver Wire 1mm ID x 70mm Long Silicone Tubing 1-HLT-70
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assemblies 1-HLA-003
Adapter For BNC Holders To U-Type Headstages Connects BNC holders to CV and HS headstages with threaded collets (U-type) 1-HLB-U
Right-Angle Adapter for HL-U Electrode Holders Fits CV and HS headstages with threaded collets (U-type) 1-HLR-U

Model Cells

Description Details Part Number
Model Cell for Oocytes Axoclamp/ GeneClamp model cell for oocytes. Connects to U-type HS series headstages 1-MCO-2U
Model Cell for TEVC / DSEVC Axoclamp/ GeneClamp model cell for two-electrode voltage clamp/ discontinuous single-electrode voltage-clamp conditions. Connects to U-type HS series headstages 1-CLAMP-1U
Model Cell for Whole Cell/ Single Channels Axopatch/ GeneClamp/ MultiClamp model cell for whole-cell / single-channel patch-clamp conditions. Connects to U-type CV series headstages 1-PATCH-1U
Model Cell for Bilayers Axopatch/ GeneClamp/ MultiClamp model cell for bilayer conditions. Connects to U-type CV series headstages 1-MCB-1U

Cables

Description Details Part Number
Cable To Connect Axoclamp 2 Headstages To Axoclamp 900A Amplifier Allows Axoclamp 2 headstages (HS-2, VG-2) to be used on Axoclamp 900A Amplifiers 1-2100-0934

Miscellaneous

Description Details Part Number
SoftPanel Amplifier Control Unit Provides physical knob and button control for computer-controlled Axoclamp 900-series and MultiClamp 700-series amplifiers. Requires a USB connection. 1-SOFTPANEL (USB)
Remote Buzz For Axoclamp 900A Hand-held buzz duration control for Axoclamp 900A Amplifier (1-50ms) 1-2950-0366
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assembly and Ag wire 1-HLA-003

Related Products & Services of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Featured Applications

Customer Breakthrough

Allegheny College uses Axon Patch-Clamp to Investigate Amyloid Beta Peptide Block Ion Channels in Alzheimer's Disease
Allegheny College uses Axon Patch-Clamp to Investigate Amyloid Beta Peptide Block Ion Channels in Alzheimer's Disease

SUCCESS STORY

Allegheny College uses our Axon patch-clamp instruments to investigate how amyloid beta peptide block ion channels in Alzheimer's disease

Investigating how amyloid beta peptide blocks io channels in Alzheimer's disease

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.