Grow, Image, and Analyze Organoids — Reliably and at Scale
End-to-end solutions for organoid development, long-term imaging, AI-driven analysis, and automated workflows—from early model development to high-throughput studies.
Welcome to the Organoid Innovation Center
The Organoid Innovation Center is a centralized resource designed to help scientists grow robust organoids, monitor their development over time, analyze complex 3D biology, and scale workflows through automation.
Need help viewing or growing robust organoids?
Learn about imaging, analysis, and automated cell culture solutions.
Looking for ready-to-use or pre-optimized organoid model systems?
Learn about Kool-Aid, DILI, and Cellesce expansion services.
How can we help you today?
Choose a workflow outcome to get recommended solutions.
Build High-Quality Organoid Models
Optimize differentiation and expansion from 2D precursor cultures to create reproducible, scalable organoids.
Monitor Organoid Growth Over Time
Capture long-term, non-invasive imaging across single or multiple plates with walk-away operation.
Analyze Organoid Structure and Function
Quantitatively measure morphology, growth, and response using AI-driven image analysis.
Scale and Automate Organoid Workflows
Reduce variability and increase throughput with automated handling, incubation, and imaging.
End-to-End Organoid Workflow
Visual: Grow & Differentiate → Image Over Time → Analyze with AI → Scale & Automate
Our solutions are designed to work together across the entire organoid lifecycle—helping you move from model development to actionable insight with confidence.
Solutions Enabled by the Organoid Innovation Center
Automated Cell Culture
Standardize feeding, passaging, and culture maintenance.
Custom Automated Workflows
Scale studies by connecting handling, incubation, imaging, and analysis
End-to-End Workflow Support
Connect assays, equipment, analysis, and expertise
Ready-to-Use and Pre-Optimized Organoid Model Systems
Accelerate your program with organoid model systems and expansion services designed to reduce startup time and improve reproducibility.
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Kool-Aid
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DILI
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Cellesce expansion services
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Trusted by Scientists Advancing Organoid Research
Researchers rely on Molecular Devices technologies to improve organoid reproducibility, scalability, and data quality across basic research, disease modeling, and drug discovery.
FAQ - Get answers to common questions about organoid culture, imaging, analysis, automation, and workflow optimization.
What are organoids used for?
Organoids are three-dimensional cell models used for disease modeling, drug discovery, toxicity testing, stem cell research, and personalized medicine.
Because they more closely mimic human biology than traditional 2D cultures, organoids can provide more predictive insights into treatment response. Molecular Devices supports organoid research with solutions for automated culture, high-content imaging, AI-powered analysis, and laboratory automation.
How are organoids grown in the laboratory?
Organoids are typically grown from stem cells or primary tissues under conditions that promote self-organization into three-dimensional structures.
Successful organoid culture depends on maintaining consistent growth conditions, environmental control, and standardized workflows. Molecular Devices helps researchers improve consistency and scalability through automated cell culture solutions.
What is the difference between organoids and spheroids?
Organoids are complex tissue-like models that mimic aspects of organ structure and function, while spheroids are simpler three-dimensional cell aggregates.
Spheroids are commonly used for cancer research and drug screening, whereas organoids provide more physiologically relevant models for studying biology and disease. Molecular Devices offers imaging and analysis solutions for both applications.
What are the biggest challenges in organoid research?
The biggest challenges in organoid research include reproducibility, culture variability, imaging complexity, scalability, and data analysis.
As organoid studies grow in size and duration, maintaining consistent experimental conditions becomes increasingly difficult. Molecular Devices helps address these challenges through integrated workflows for automated culture, imaging, analysis, and laboratory automation.
How can researchers improve organoid reproducibility?
Researchers can improve organoid reproducibility by standardizing culture conditions, reducing manual handling, and automating routine workflows.
Variability introduced during feeding, passaging, and maintenance can significantly impact results. The CellXpress.ai™ Automated Cell Culture System helps standardize these processes to improve consistency across experiments.
What is the best way to image organoids?
Longitudinal, non-invasive imaging is the most effective way to monitor organoid growth, morphology, and treatment response over time.
Capturing organoid development throughout an experiment provides deeper biological insights than endpoint measurements alone. ImageXpress® high-content imaging systems enable automated organoid imaging with environmental control for long-term studies.
How do you analyze organoid morphology?
Organoid morphology is analyzed by measuring features such as size, shape, growth, viability, and structural complexity.
Automated image analysis improves consistency and scalability while reducing manual interpretation. IN Carta® Image Analysis Software uses AI-powered tools to segment, quantify, and analyze organoid phenotypes across experiments.
How can organoid workflows be automated?
Organoid workflows can be automated by integrating culture, media exchange, incubation, imaging, and analysis into a streamlined process.
Automation improves consistency, increases throughput, and reduces time spent on repetitive laboratory tasks. Molecular Devices provides scalable automation solutions that support organoid workflows from culture through high-throughput screening.
PREV CONTENT
Automated 3D cell culture and image analysis lab streamlines and scales complex biology research
The new Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel 3D biology methods to address key challenges of scaling complex 3D biology.
The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house scientists.
An end-to-end solution standardizes the organoid development process with cell culture, treatment, and incubation, through to imaging, analysis, and data processing, delivering consistent, unbiased, and biologically-relevant results at scale.
Quickly adopt innovative, 3D biological methods and technologies for drug discovery
The center expands beyond imaging to demonstrate a fully-integrated solution that addresses the challenges associated with every step in the sample prep-to-report pipeline for assays performed on complex 3D biological models.
The Organoid Innovation Center showcases cutting-edge instruments that work harmoniously together for autonomous, long-term, live cell 2D and 3D cell culture growth and monitoring with intelligent label-free imaging. This integrated workflow provides quality control alerts and readiness, 3D organoid screening, and deep learning image analysis that reveals hidden patterns other technologies miss.
See the power and flexibility of an automated and customizable high-throughput screening solution
With intuitive scheduling software researchers can control the 3D workflow remotely, tracking the cell journey from single cell to differentiated organoid, along the way. Cell culture and incubation is streamlined with an automated incubator and collaborative robot that maintains culture consistency. Media exchange for culture maintenance is standardized and streamlined with automated liquid handling, minimizing manual intervention. 3D model development can be monitored over time with label-free imaging to assess assay readiness – and with real-time feedback, scheduling of automated compound addition and treatment is standardized.
Organoid Screening Workflow
- Step 1) 2d Pre-culture – Organoids are pre-cultured from iPSC-derived or primary cells (intestine, lung, or brain)
- Step 2) Developing 3D Organoids – Cells are transferred to 24-well plates then placed in an incubator to promote cell growth and differentiation into specific tissue in 3D
- Step 3) Organoid Culture – Organoid culture process require multiple steps with different media exchanges
- Step 4) Monitor organoid growth & development – Organoid are monitored and characterized for complex analysis of tissue structure and differentiation
- Step 5) Confocal imaging and 3D analysis – The visualization and characterization of multiple quantitative descriptors used for studying disease phenotypes and compound effects
Our new ImageXpress Confocal HT.ai High-Content Imaging system is designed for 3D imaging. This system offers eight high-powered laser excitation channels and automated water immersion objectives that boost signal and assay sensitivity without sacrificing speed. Spinning disk confocal technology with five pinhole geometry options reduces haze from out-of-focus light for deeper organoid penetration and improved axial resolution. For analysis of complex 3D biology, IN Carta Image Analysis Software provides a streamlined workflow with powerful deep-learning-based segmentation, machine learning-based classification, and 3D volumetric analysis.
Complete solutions for a fully integrated lab automation workflow
Our lab automation solutions include scientists and engineers who can customize our instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. From incubators, liquid handlers, and robotics to customized software and hardware—and with over 35 years of experience in the life science industry—you can count on us to deliver quality products and provide worldwide support.
Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms
Molecular Devices is honored to be designated as a "Lab of the Future" Company
Exhibitors that received the SLAS2024 Lab of the Future designation have demonstrated their ability to provide solutions that go beyond automated instrumentation alone to push the boundaries of current technology and break new ground in achieving complete integration of automated workflows in the lab space.
Take a step ‘back to the future’ and revisit us at SLAS 2024 – explore six posters that feature this groundbreaking innovation and unveil the latest advancements in laboratory research and technology.
Lung organoid cell image gallery
Image analysis of organoid
Lung organoids
Lung organoids 2
Automated imaging of live organoids in Matrigel was done using confocal option, 4x or 10x magnification
High complexity lung organoid
Gut organoid dTomato cells in red Detection of Double positive(mNeon) in green
Internally expressed genes dTomato magenta mNeon green 10x
Intestinal organoid 40x,dTomato cell count
Lung organoid 10x 20x Multiple stains Hoechst MitoTracker
Lung organoids,projection image and a panel with Z stack; live stained with Hoechstblue,nuclei),MitoTracker(red,mitochondria),and Calcein AM(green,viability dye);20x water immersion
Lung organoids 3
Lung organoids 4
Lung organoids lung airway epithelial cells cultured in matrigel domes for 8 weeks
Organoid analysis multiplexed cell scoring
Organoid analysis multiplexed cell scoring 2
Organoid development
Applications and assays
The Organoid Innovation Center builds on Molecular Devices 35 years of experience delivering high-performance life science technology to customers for improved drug development, biotechnology research, and clone screening workflows.
Learn more about our industry leading 3D biology and automated high-content imaging applications:
Latest Resources
Not Sure Where to Start?
Our organoid workflow experts can help you identify the right tools and strategies based on your research goals, throughput needs, and level of automation.