Overview of Western Blot Cartridge

The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3x and SpectraMax Paradigm Multi-Mode Platforms. Leveraging this novel technology, researchers can:

  • Add western blot detection to their microplate reader within 2 minutes
  • Eliminate time-dependent substrate addition steps
  • Maintain femtogram to picogram protein sensitivity comparable to traditional western blot methods
  • Sustain blot signal stability for up to 30 days
  • Use a single software platform SoftMax Pro Software, to run both microplate and western blot detection assays

Technologies of Western Blot Cartridge

  • Schematic representation of emission lifetimes that minimize background fluorescence

    Schematic representation of emission lifetimes that minimize background fluorescence

    Schematic representation of emission lifetimes of background fluorescence and Eu-chelate showing principle of TRF measurement. Signal is measured after appropriate time-delay to reduce auto-fluorescence.

  • ScanLater Western Blot Assay Workflow

    ScanLater Western Blot Assay Workflow

  • ScanLater Western Blot Assay Principle

    ScanLater Western Blot Assay Principle

    Schematic of protein detection method shows the secondary antibody is directly labeled with Europium.

  • Europium-chelate excitation and emission spectra

    Europium-Chelate Excitation and Emission Spectra

    Schematic representation of Europium-chelate excitation and emission spectra

Resources of Western Blot Cartridge

Videos

ScanLater Western Blot Detection Cartridge

ScanLater Western Blot Detection Cartridge

ScanLater Western Blot Detection Cartridge

Product Name Description Part Number
System
ScanLater Western 
Blot Detection System
  • Membrane sample holder
  • SoftMax Pro Software 6.3.1
  • Image plug-in for data analysis
0200-7027
Assay Kit
ScanLater: Eu-Streptavidin
Evaluation Kit
  • Europium-labeled streptavidin (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8200

Click here to purchase

Assay Kit
ScanLater: Eu-Streptavidin
Bulk Kit
  • Europium-labeled streptavidin (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8203

Click here to purchase

Assay Kit
ScanLater: Anti-Mouse
Evaluation Kit
  • Europium-labeled goat anti-mouse antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8201

Click here to purchase

Assay Kit
ScanLater: Anti-Mouse
Bulk Kit
  • Europium-labeled goat anti-mouse antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8205

Click here to purchase

Assay Kit
ScanLater: Anti-Rabbit
Evaluation Kit
  • Europium-labeled goat anti-rabbit antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8202

Click here to purchase

Assay Kit
ScanLater: Anti-Rabbit
Bulk Kit
  • Europium-labeled goat anti-rabbit antibody (60 μg)
  • Blocking buffer (120 mL), Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8204

Click here to purchase

Assay Kit
ScanLater: Anti-Goat
Evaluation Kit
  • Europium-labeled donkey anti-goat antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8224

Click here to purchase

Assay Kit
ScanLater: Anti-Goat
Bulk Kit
  • Europium-labeled donkey anti-goat antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8227

Click here to purchase

Assay Kit
ScanLater: Anti-Rat
Evaluation Kit
  • Europium-labeled donkey anti-rat antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8225

Click here to purchase

Assay Kit
ScanLater: Anti-Rat
Bulk Kit
  • Europium-labeled donkey anti-rat antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8228

Click here to purchase

Protein Ladder
ScanLater Western Blot Protein Ladder
  • 7 biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD
  • 3 pre-stained marker proteins at 18, 31, and 70 kD
  • Ready to use, for 30 mini gels with recommended
    protocol
R8220

Click here to purchase

Assay Kit Components
ScanLater Wash Buffer (100 mL) R8206
ScanLater 5x Blocking Buffer (120 mL) R8207
ScanLater Goat Anti-Mouse Eu-labeled Antibody (60 µg) R8208
ScanLater Goat Anti-Rabbit Eu-labeled Antibody (60 µg) R8209
ScanLater Eu-labeled Antibody Streptavidin (60 µg) R8212
ScanLater Eu-Anti-Goat IgG (60 μg) R8226

Compatible Products & Services of Western Blot Cartridge

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.