Luminescence-based assays often provide greater sensitivity than absorbance or fluorescence. Luminescence single-mode microplate readers (also called microplate luminometers) are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. ‘Glow’ luminescence assays with long-lived signal can be detected by any luminescence reader, while ‘flash’ assays with short-lived signal require a microplate reader equipped with injectors.
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REACTIVE OXYGEN SPECIES (ROS)
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems...
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