Fluorescence detection typically offers greater sensitivity and a wider dynamic range compared to absorbance. Because many reagents can be fluorescently labeled, fluorescence single-mode microplate readers (also referred to as microplate spectrofluorometers) are used to detect many biological and chemical processes. There are often fluorescent alternatives for applications like cell viability and nucleic acid quantification.
Find an extensive collection of application notes, research, and technology related to fluorescence microplate reader assays and applications.
Nanoorange protein quantitation
This application note describes how use the NanoOrange® Protein Quantitation Kit from Life Technologys in SpectraMax® microplate readers with the fluorescence detection mode and..
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