Fluorescence Single-Mode Microplate Readers

Fluorescence detection technology

Fluorescence detection typically offers greater sensitivity and a wider dynamic range compared to absorbance. Because many reagents can be fluorescently labeled, fluorescence single-mode microplate readers (also referred to as microplate spectrofluorometers) are used to detect many biological and chemical processes. There are often fluorescent alternatives for applications like cell viability and nucleic acid quantification.

How does fluorescence detection work?
A microplate reader with fluorescence intensity (FI) detection uses a light source to excite a fluorophore (fluorescent molecule) at a particular wavelength, which is selected by a filter or monochromator. The fluorophore then emits light of a different wavelength, also selected by a filter or monochromator. This emitted fluorescence is detected by a photomultiplier tube (PMT), and the fluorescence intensity of the sample is expressed as relative fluorescence units.
What’s the difference between a fluorescence spectrophotometer and a fluorescence plate reader?
There are many terms that refer to instruments used to measure the fluorescence of a sample: fluorescence spectrophotometer, fluorescence spectrometer, spectrofluorometer, fluorometer, fluorimeter, etc. In general, they use a light source to excite the sample at a certain wavelength and measure the emitted fluorescence at a second wavelength. The sample may be held in many formats, including cuvettes, capillaries, petri dishes, etc. ‘Fluorescence microplate reader’ refers to an instrument that can measure the fluorescence of samples in a microplate, most often a 96- or 384-well plate. Microplates offer higher throughput and are useful for screening or other applications where many samples must be analyzed.
What’s a dual monochromator spectrofluorometer system?
Some fluorescence single-mode microplate readers use dual monochromators instead of filters to select the wavelength of light that excites the sample, as well as the wavelength that is emitted by the sample. Monochromators use a diffraction grating to spatially separate the colors of light and can select a wide range of wavelengths without the need to install a separate filter for each wavelength required by an application.

Fluorescence measurement applications and research

Find an extensive collection of application notes, research, and technology related to fluorescence microplate reader assays and applications.

  • Calcium assays
  • cAMP Detection
  • Caspase-3
  • CyQUANT
  • Cytotoxicity assays
  • EarlyTox Live Cell (Viability)
  • Fluorometric protease assays
  • Green fluorescent protein
  • Molecular beacons
  • NanoOrange Protein Quantitation
  • Nucleic Acid Detection
  • Pico/Ribo/Oli Green
  • PicoGreen DNA detection
  • Tryptophan detection
More Application notes

SpectraMax Gemini XPS/EM Microplate Readers

Fluorescence detection without filters

  • No fliters needed
  • High level of sensitivity
  • Validation tools
  • Robotics compattible
Learn more

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