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Microscopy imaging for cell counting through to complex image analysis

The CellReporterXpress Automated Image Acquisition and Analysis Software works with the ImageXpress® Pico systems. It has a simple, easy-to-learn interface for performing quantitative analysis on images acquired from automated microscopy. The software enables distributed analysis of images for increased throughput and is ideal for scaling your digital microscopy imaging with slides or microplates. An icon-driven, linear workflow with a range of predefined protocols provides a streamlined user experience. With features such as Digital Confocal 2D on-the-fly image deconvolution, and Live Preview advanced region of interest identification, you can save time while improving assay quality and significantly increasing resolution.

  • Analyze during acquisition

    Analyze during acquisition

    Reduce the time to run experiments with our integrated on-the-fly image analysis capability, allowing you to view numeric data during acquisition. In addition, our Digital Confocal option performs 2D image deconvolution in real-time allowing you to significantly increase resolution and assay quality.

  • Protocols

    Simplify image analysis

    Predefined analysis protocols for label-free and fluorescence imaging provides you with the ability to quickly run common biological assays while still allowing you to create and save your own protocols. With optimized templates ranging from simple cell counting to complex neurite tracing, and including custom 2 and 3 lane Mimetas protocols, users no longer need to worry about adjusting complicated parameters or exporting to third-party software for robust analysis.

  • Visualize results differently

    Visualize results differently

    The software allows users to visualize their data in a variety of formats including whole plate, slide, or individual cell views. You can view populations of cells across the entire well in scatter plot, table, or histogram. In addition, the Live Preview function simplifies identification of regions of interest, letting users pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort.

ImageXpress Pico Automated Cell Imaging System-Interactive Demo

Features

  • Region selection for acquisition

    Multiple unique positions within a well or slide can be selected to acquire and analyze only key areas of interest.

  • Label-free analysis

    Automatically identifies objects in brightfield including small round cells, large cells, and beads.

  • Click-to-find feature

    This feature auto calculates object parameters based on user-selected cells of interest, simplifying analysis setup for cell counting and for cellular responses.

  • Side-by-side cell magnification

    Zoom or pan high-resolution images from whole well view down to cell level view. Simultaneously compare images from two wells.

  • Touch screen capability

    Exceptional usability and precise control is built-in whether using a personal computer or a tablet.

  • Browser-based software

    The ImageXpress Pico system can be controlled through a browser with data from any computer on the user’s network via Chrome™ or Safari®.

Save time and reduce errors with preconfigured analysis protocols

 

Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis. With features like the click-to-find tool, analysis parameters can be optimized by simply clicking on a few cells that match specific criteria.
 

Click on the icons below for additional information on each of the CellReporterXpress capabilities
 

CellReporterXpress is part of the ImageXpress Pico Automated Cell Imaging System. To learn more, please visit the ImageXpress Pico product page.

ImageXpress Pico Automated Cell Imaging System

  • Ideal for cell counting, transfection efficiency, and cell health assays
  • 25+ preconfigured application protocols
  • 3D z-stack acquisition
  • Digital Confocal 2D on-the-fly image deconvolution
  • Live Preview
  • Browser-based, Tablet and touchscreen compatible software

Learn more

ImageXpress Pico Automated Cell Imaging System

  • Live Cell Imaging

    Live Cell Imaging

    Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.

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Resources of CellReporterXpress Image Acquisition and Analysis Software

Presentations
Videos & Webinars
Antigen / Immunogen Discovery and Optimization

Immunology and Vaccine Development Workflow

Hybridoma Workflow

Hybridoma Workflow

Preview on Imagexpress Pico

How to easily image slides and regions of interest with Live Preview on the ImageXpress Pico

Environmental control settings in ImageXpress Pico

Configuring environmental control settings on the ImageXpress Pico

z-stack image acquisition using CellReporterXpress

How to perform a z-stack image acquisition using CellReporterXpress

Setting up acquisition and analysis

Setting up acquisition and analysis on the ImageXpress Pico

Transmitted light cell scoring

Transmitted light cell scoring on the ImageXpress Pico

Reviewing Experiments on ImageXpress Pico

Reviewing completed experiments on the ImageXpress Pico

ImageXpress Pico Automated Cell Imaging System

ImageXpress Pico Automated Cell Imaging System

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Evaluation of mitochondrial integrity and mitochondria membrane potential using automated cell imaging and analysis

    Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP)1,2. Mitochondrial depolarization is an early signal for hypoxic damage or oxidative stress. Cationic fluorescent dyes are commonly used tools to assess MMP. We performed a short-term (60 min) compound treatment… View more

    Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP)1,2. Mitochondrial depolarization is an early signal for hypoxic damage or oxidative stress. Cationic fluorescent dyes are commonly used tools to assess MMP. We performed a short-term (60 min) compound treatment with two known inhibitors of oxidative phosphorylation, Antimycin A and CCCP.

    Contributors: Matthew Hammer, Oksana Sirenko  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Analysis of apoptosis using automated cell imaging

    Apoptosis is a process of programmed cell death that occurs in multicellular organisms.1,2 Biochemical events lead to characteristic changes in cell morphology and cell death. Morphological changes include cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and mRNA decay. Apoptosis is a highly regulated… View more

    Apoptosis is a process of programmed cell death that occurs in multicellular organisms.1,2 Biochemical events lead to characteristic changes in cell morphology and cell death. Morphological changes include cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and mRNA decay. Apoptosis is a highly regulated process that can be initiated via the intrinsic pathway as a response to various stressors including starvation, infection, hypoxia, oxidative stress, etc. Mitochondria damage plays an important role in the initiation of apoptosis. In the extrinsic pathway, apoptosis is initiated by external signals, including tumor necrosis factor receptor family-mediated signaling. Both pathways induce cell death by activating caspase enzymes.

    Contributors: Matthew Hammer, Oksana Sirenko  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices | Sunnyvale, CA

    Detection of autophagy using automated imaging

    Autophagy is a highly regulated process of degrading and recycling damaged proteins and organelles in response to cellular stress.1, 2 It is mediated by a unique vesicle called the autophagosome, which is assembled by the formation of a double membrane around the cellular component marked for destruction.1 The autophagosome vesicle then fuses with… View more

    Autophagy is a highly regulated process of degrading and recycling damaged proteins and organelles in response to cellular stress.1, 2 It is mediated by a unique vesicle called the autophagosome, which is assembled by the formation of a double membrane around the cellular component marked for destruction.1 The autophagosome vesicle then fuses with the lysosome to deliver its contents for degradation by lysosomal hydrolases.

    Contributors: Oksana Sirenko  
    Go to article

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