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Microscopy imaging for cell counting through to complex image analysis

The CellReporterXpress Automated Image Acquisition and Analysis Software works with the ImageXpress® Pico system. It has a simple, easy-to-learn interface for performing quantitative analysis on images acquired from automated microscopy. The software enables distributed analysis of images for increased throughput and is ideal for scaling your digital microscopy imaging with slides or microplates. An icon-driven, linear workflow with a range of predefined protocols provides a streamlined user experience. With features such as Automated focus algorithms for a variety of applications including custom organ-on-a-chip plates, Digital Confocal 2D on-the-fly image deconvolution, and Live Preview advanced region of interest identification, you can save time while improving assay quality and significantly increasing resolution.

  • Analysis Icon

    Analyze during acquisition

    Reduce the time to run experiments with our integrated on-the-fly image analysis capability, allowing you to view numeric data during acquisition. In addition, our Digital Confocal option performs 2D image deconvolution in real-time allowing you to significantly increase resolution and assay quality.

  • Protocols Icon

    Simplify image analysis

    Predefined analysis protocols for label-free and fluorescence imaging provides you with the ability to quickly run common biological assays while still allowing you to create and save your own protocols. With optimized templates ranging from simple cell counting to complex neurite tracing, and including custom 2 and 3 lane Mimetas protocols, users no longer need to worry about adjusting complicated parameters or exporting to third-party software for robust analysis.

  • Share and access data from anywhere

    Share and access data from anywhere

    The software makes sharing, presenting, and collaborating with peers easier, allowing multiple users to utilize the software simultaneously through browser-based remote access.

See what’s new with CellReporterXpress

Features

  • preconfigured application protocols

    +25 preconfigured application protocols

    User guided artificial intelligence routines optimize parameters automatically resulting in robust, easy-to-use analysis protocols.

  • Automated focusing routines for a variety cell-based imaging applications

    Automated focusing routines for a variety cell-based imaging applications

    Provides reliable focusing across all of your labware to ensure high-quality imaging for a wide variety of sample types.

  • Picks Icon

    Region selection for acquisition

    Multiple unique positions within a well or slide can be selected to acquire and analyze only key areas of interest.

  • Analysis Icon

    Label-free analysis

    Automatically identifies objects in brightfield including small round cells, large cells, and beads.

  • Clicks Icon

    Click-to-find feature

    This feature auto calculates object parameters based on user-selected cells of interest, simplifying analysis setup for cell counting and for cellular responses.

  • Accuracy Icon

    Side-by-side cell magnification

    Zoom or pan high-resolution images from whole well view down to cell level view. Simultaneously compare images from two wells.

  • Touchscreen Icon

    Touch screen capability

    Exceptional usability and precise control is built-in whether using a personal computer or a tablet.

  • Software Icon

    Browser-based software

    The ImageXpress Pico system can be controlled through a browser with data from any computer on the user’s network via Chrome™ or Safari®.

Intelligent image acquisition and analysis

 

CellReporterXpress software, along with the ImageXpress Pico system, does more than imaging—it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays.

Autofocus

Autofocus

Automated focus algorithms for a variety of applications

Two robust autofocus mechanisms: Detect Surface for hardware autofocus, and Find Best Plane for image-based autofocus. Provides reliable focusing across all of your labware to ensure high-quality imaging for a wide variety of sample types.

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Digital Confocal

digital-confocal

Increase resolution with on-the-fly deconvolution

Enhance contrast of images during acquisition with the Digital Confocal* 2D on-the-fly deconvolution option, allowing you to increase resolution and improve assay quality.

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Live Preview

Live Preview

Identify regions of interest quickly and easily

Live Preview simplifies identification of regions of interest, letting you pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort.

Environmental Control

Environmental Control

Monitor live cell assays with on-board environmental control

Multi-day, time-lapse, and live cell assays can be run using the on-board environmental system with options for humidity, CO2, and O2 control. Optimized to prevent Z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions.

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Multi-wavelength Cell Scoring

Multi-wavelength Cell Scoring

 

Features multi-wavelength cell scoring with up to four fluorescent stains

The preconfigured protocol is ideal for counting and logging measurements of cells in multiple wavelength experiments. Using a fluorescent marker for the nucleus and additional markers for the cytoplasm, each wavelength is analyzed and cells are assigned multiparametric phenotypic profiles.

Z-stack Acquisition

Z-stack Acquisition

Capture deeper insights with Z-stack acquisition

Generate sharper images for more accurate segmentation using Z -stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection.

 

Save time and reduce errors with preconfigured analysis protocols

 

Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis. With features like the click-to-find tool, analysis parameters can be optimized by simply clicking on a few cells that match specific criteria.
 

Click on the icons below for additional information on each of the CellReporterXpress capabilities
 

CellReporterXpress is part of the ImageXpress Pico Automated Cell Imaging System. To learn more, please visit the ImageXpress Pico product page.

Digital microscopy with automated brightfield, fluorescence, and Digital Confocal imaging

  • Ideal for cell counting, transfection efficiency, and cell health assays
  • Hardware autofocus uses an LED beam to find reflective surfaces and is designed for speed
  • Monitor live cell assays with on-board environmental control
  • Browser-based, tablet and touchscreen compatible software
  • Tailored laboratory automation solutions with robotics, incubators, and software

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ImageXpress Pico Automated Cell Imaging System

  • Live Cell Imaging

    Live Cell Imaging

    Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.

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Resources of CellReporterXpress Image Acquisition and Analysis Software

Presentations
Videos & Webinars
Antigen / Immunogen Discovery and Optimization

Immunology and Vaccine Development Workflow

Hybridoma Workflow

Hybridoma Workflow

Preview on Imagexpress Pico

How to easily image slides and regions of interest with Live Preview on the ImageXpress Pico

Environmental control settings in ImageXpress Pico

Configuring environmental control settings on the ImageXpress Pico

z-stack image acquisition using CellReporterXpress

How to perform a z-stack image acquisition using CellReporterXpress

Setting up acquisition and analysis

Setting up acquisition and analysis on the ImageXpress Pico

Transmitted light cell scoring

Transmitted light cell scoring on the ImageXpress Pico

Reviewing Experiments on ImageXpress Pico

Reviewing completed experiments on the ImageXpress Pico

ImageXpress Pico Automated Cell Imaging System

ImageXpress Pico Automated Cell Imaging System

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Evaluation of mitochondrial integrity and mitochondria membrane potential using automated cell imaging and analysis

    Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP)1,2. Mitochondrial depolarization is an early signal for hypoxic damage or oxidative stress. Cationic fluorescent dyes are commonly used tools to assess MMP. We performed a short-term (60 min) compound treatment… View more

    Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP)1,2. Mitochondrial depolarization is an early signal for hypoxic damage or oxidative stress. Cationic fluorescent dyes are commonly used tools to assess MMP. We performed a short-term (60 min) compound treatment with two known inhibitors of oxidative phosphorylation, Antimycin A and CCCP.

    Contributors: Matthew Hammer, Oksana Sirenko  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Analysis of apoptosis using automated cell imaging

    Apoptosis is a process of programmed cell death that occurs in multicellular organisms.1,2 Biochemical events lead to characteristic changes in cell morphology and cell death. Morphological changes include cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and mRNA decay. Apoptosis is a highly regulated… View more

    Apoptosis is a process of programmed cell death that occurs in multicellular organisms.1,2 Biochemical events lead to characteristic changes in cell morphology and cell death. Morphological changes include cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and mRNA decay. Apoptosis is a highly regulated process that can be initiated via the intrinsic pathway as a response to various stressors including starvation, infection, hypoxia, oxidative stress, etc. Mitochondria damage plays an important role in the initiation of apoptosis. In the extrinsic pathway, apoptosis is initiated by external signals, including tumor necrosis factor receptor family-mediated signaling. Both pathways induce cell death by activating caspase enzymes.

    Contributors: Matthew Hammer, Oksana Sirenko  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices | Sunnyvale, CA

    Detection of autophagy using automated imaging

    Autophagy is a highly regulated process of degrading and recycling damaged proteins and organelles in response to cellular stress.1, 2 It is mediated by a unique vesicle called the autophagosome, which is assembled by the formation of a double membrane around the cellular component marked for destruction.1 The autophagosome vesicle then fuses with… View more

    Autophagy is a highly regulated process of degrading and recycling damaged proteins and organelles in response to cellular stress.1, 2 It is mediated by a unique vesicle called the autophagosome, which is assembled by the formation of a double membrane around the cellular component marked for destruction.1 The autophagosome vesicle then fuses with the lysosome to deliver its contents for degradation by lysosomal hydrolases.

    Contributors: Oksana Sirenko  
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