The CellReporterXpress Automated Image Acquisition and Analysis Software works with the ImageXpress® Pico system. It has a simple, easy-to-learn interface for performing quantitative analysis on images acquired from automated microscopy. The software enables distributed analysis of images for increased throughput and is ideal for scaling your digital microscopy imaging with slides or microplates. An icon-driven, linear workflow with a range of predefined protocols provides a streamlined user experience. With features such as Automated focus algorithms for a variety of applications including custom organ-on-a-chip plates, Digital Confocal 2D on-the-fly image deconvolution, and Live Preview advanced region of interest identification, you can save time while improving assay quality and significantly increasing resolution.
Reduce the time to run experiments with our integrated on-the-fly image analysis capability, allowing you to view numeric data during acquisition. In addition, our Digital Confocal option performs 2D image deconvolution in real-time allowing you to significantly increase resolution and assay quality.
Predefined analysis protocols for label-free and fluorescence imaging provides you with the ability to quickly run common biological assays while still allowing you to create and save your own protocols. With optimized templates ranging from simple cell counting to complex neurite tracing, and including custom 2 and 3 lane Mimetas protocols, users no longer need to worry about adjusting complicated parameters or exporting to third-party software for robust analysis. With the addition of IN Carta® Image Analysis Software, you can leverage machine learning to get more information and increase accuracy in the analysis of your data to enable new discoveries with confidence.
The software makes sharing, presenting, and collaborating with peers easier, allowing multiple users to utilize the software simultaneously through browser-based remote access.
User guided artificial intelligence routines optimize parameters automatically resulting in robust, easy-to-use analysis protocols.
Provides reliable focusing across all of your labware to ensure high-quality imaging for a wide variety of sample types.
Multiple unique positions within a well or slide can be selected to acquire and analyze only key areas of interest.
Automatically identifies objects in brightfield including small round cells, large cells, and beads.
This feature auto calculates object parameters based on user-selected cells of interest, simplifying analysis setup for cell counting and for cellular responses.
Zoom or pan high-resolution images from whole well view down to cell level view. Simultaneously compare images from two wells.
Exceptional usability and precise control is built-in whether using a personal computer or a tablet.
The ImageXpress Pico system can be controlled through a browser with data from any computer on the user’s network via Chrome™ or Safari®.
CellReporterXpress software, along with the ImageXpress Pico system, does more than imaging—it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays.
Automated focus algorithms for a variety of applications
Two robust autofocus mechanisms: Detect Surface for hardware autofocus, and Find Best Plane for image-based autofocus. Provides reliable focusing across all of your labware to ensure high-quality imaging for a wide variety of sample types.
Increase resolution with on-the-fly deconvolution
Enhance contrast of images during acquisition with the Digital Confocal* 2D on-the-fly deconvolution option, allowing you to increase resolution and improve assay quality.
Identify regions of interest quickly and easily
Live Preview simplifies identification of regions of interest, letting you pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort.
Monitor live cell assays with on-board environmental control
Multi-day, time-lapse, and live cell assays can be run using the on-board environmental system with options for humidity, CO2, and O2 control. Optimized to prevent Z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions.
Features multi-wavelength cell scoring with up to four fluorescent stains
The preconfigured protocol is ideal for counting and logging measurements of cells in multiple wavelength experiments. Using a fluorescent marker for the nucleus and additional markers for the cytoplasm, each wavelength is analyzed and cells are assigned multiparametric phenotypic profiles.
Capture deeper insights with Z-stack acquisition
Generate sharper images for more accurate segmentation using Z -stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection.
Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis. With features like the click-to-find tool, analysis parameters can be optimized by simply clicking on a few cells that match specific criteria.
Click on the icons below for additional information on each of the CellReporterXpress capabilities
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells.
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.
High-content imaging (a branch of microscopy aimed at capturing images at high-throughput) usually involves producing thousands or millions of images in one data-acquisition round fo…
3D bioprinting is defined as the additive deposition of cells and biocompatible materials to build biologically functional 3D structure or artificial tissue models
The intestinal crypt system is an important part of the intestinal organoid where mature organoids have more complex and numerous crypt structures. Yet, due to their high turnover rate…
From customer feedback to workflow improvements The path to understanding complex biological processes and diseases is paved with a lot of challenges. As the desired level of…
If you didn't get a chance to visit us at our poster sessions during ISSCR 2021, don’t fret. We've gathered all our sessions right here for you. The ISSCR Annual Meeting brought…
The CellReporterXpress® Automated Image Acquisition and Analysis Software works with the ImageXpress® Pico Automated Cell Imaging System. It has a clean, easy-to-learn interface for…
From endpoint and live-cell imaging applications to imaging and analyzing 2D monolayers, 3D cell models, and organ-on-a-chip platforms, the world of cellular imaging is very diverse.…
Environmental control mimics the cell environment and enables you to run multi-day studies, time-lapse, and live cell assays. With CellReporterXpress™ software, the ImageXpress® Pico…
There have been significant advancements in microscopy and camera technology, as well as advancements in technologies for labeling molecules of interest over the past decade. These…
Artificial intelligence (AI) is finding its way into many aspects of modern life, from autonomous vehicles to voice-powered personal assistants, and even the creation of art. But it…
Cheryl Bell talks about making the transition from manual microscopy to automated microscopy Our Field Applications Scientist, Cheryl Bell, was recently featured in a Women in…
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translation research. Significant progress has b…
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
For over 40 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Kayla Hill discusses the latest trends in cellular imaging We recently hosted a webinar with our Field Applications Scientist, Kayla Hill, PhD, who explored high-content analysis…
There is a growing trend to develop screening assays using three-dimensional (3D) cell culture.
With each passing day, continuous advancements are being made in the studies that cutting-edge instruments can perform and in the complexity of the biological samples that are being…
OUR ABILITY to image cells has come a long way since the pioneering days of Galileo Galilei and Antonie van Leeuwenhoek. The multiple imaging techniques available today range from si…
Depending on your application and exactly what you are researching, there are multiple imaging techniques available, ranging from confocal microscopy to electron microscopy and more…
Developments in informatics solutions and high-content imaging instruments, increased focus on cell-based research, and rising government support of research and development for drug…
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. O…
Immunology and Vaccine Development Workflow
How to easily image slides and regions of interest with Live Preview on the ImageXpress Pico
Configuring environmental control settings on the ImageXpress Pico
How to perform a z-stack image acquisition using CellReporterXpress
Setting up acquisition and analysis on the ImageXpress Pico
Transmitted light cell scoring on the ImageXpress Pico
Reviewing completed experiments on the ImageXpress Pico
ImageXpress Pico Automated Cell Imaging System
Latest Citations: For a complete list, please click here .
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP)1,2. Mitochondrial depolarization is an early signal for hypoxic damage or oxidative stress. Cationic fluorescent dyes are commonly used tools to assess MMP. We performed a short-term (60 min) compound treatment with two known inhibitors of oxidative phosphorylation, Antimycin A and CCCP.
Apoptosis is a process of programmed cell death that occurs in multicellular organisms.1,2 Biochemical events lead to characteristic changes in cell morphology and cell death. Morphological changes include cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and mRNA decay. Apoptosis is a highly regulated process that can be initiated via the intrinsic pathway as a response to various stressors including starvation, infection, hypoxia, oxidative stress, etc. Mitochondria damage plays an important role in the initiation of apoptosis. In the extrinsic pathway, apoptosis is initiated by external signals, including tumor necrosis factor receptor family-mediated signaling. Both pathways induce cell death by activating caspase enzymes.
Autophagy is a highly regulated process of degrading and recycling damaged proteins and organelles in response to cellular stress.1, 2 It is mediated by a unique vesicle called the autophagosome, which is assembled by the formation of a double membrane around the cellular component marked for destruction.1 The autophagosome vesicle then fuses with the lysosome to deliver its contents for degradation by lysosomal hydrolases.