Image deconvolution algorithms reverse out-of-focus fluorescent blurring in widefield microscopy
Widefield microscopy is a powerful tool that has been utilized to investigate cellular and molecular processes involved in cell signaling. Although fluorescent blurring from out-of-focus light is an innate result from all widefield microscopes, image deconvolution algorithms can reverse this out-of-focus light artifact.
The Digital Confocal Option is a 2D real-time deconvolution technology that helps to restore a cellular image’s resolution and contrast by mathematical deblurring of the image through reversal of the point spread function. As a result, images and fine detail become sharper while maintaining quantitative information.
Resources for Digital Confocal Option – 2D Real-Time Deconvolution
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Designed for the ImageXpress® Pico Automated Cell Imaging System, and utilizing CellReporterXpress® Image Acquisition and Analysis Software, the Digital Confocal* Option provides 2D on-…
Widefield microscopy is a powerful tool that has been utilized to investigate cellular and molecular processes involved in cell signaling.
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