This application note demonstrates how adjusting shaking intervals in microplate readers can optimize microbial growth and marker expression for diverse strains. Using the SpectraMax iD series of microplate readers with SoftMax® Pro software, researchers can easily program and automate shaking protocols, measure absorbance and fluorescence, and streamline data analysis for reliable, high-throughput results

Key Features:

• Programmable continuous or intermittent shaking protocols for microbial assays
• Advanced shaking upgrade: adjustable RPM and shaking diameter for strain-specific optimization
• SoftMax Pro workflow editor for easy setup and automation
• Simultaneous absorbance (OD600) and fluorescence measurements in one workflow
• Automated Vmax calculation and kinetic data analysis
• High-throughput capability with ready-to-run protocols

Benefits:

• Optimize growth conditions for aerobic, anaerobic, fast-growing, and fragile strains
• Improve consistency and reliability of absorbance readings by maintaining cell suspension
• Reduce artifacts and variability in clump-forming strains with intermittent shaking
• Enhance marker expression and growth phase adaptation with tailored shaking intervals
• Save time and minimize contamination risk with automated, high-throughput workflows
• Effortlessly analyze growth curves and marker expression with SoftMax Pro’s automated tools

Learn more about the systems behind the methods