APPLICATION NOTE
High-Throughput hALT Assay for 3D Liver Microtissues
Rapidly quantify liver cell viability in 3D microtissues with a sensitive, automated ELISA workflow—preserving samples for further research and enabling high-throughput drug screening.
Unlock Faster, More Informative Drug Screening with 3D Liver Culture Biomarker Analysis
- High-Throughput Efficiency: Analyze up to 384 samples in just 90 minutes using Abcam’s SimpleStep ELISA® format for human ALT (hALT)
- Preserve Sample Integrity: Non-destructive workflow keeps 3D liver microtissues intact for additional downstream studies
- Automation-Ready: Seamless integration with automated liquid handling and plate washing systems for reproducible results
- Sensitive Quantitation: Detect hALT concentrations from 100 to 7,000 pg/mL, enabling precise assessment of liver cell viability
- Rapid Data Acquisition: Simultaneous plate washing and fast multi-channel reading dramatically reduce hands-on and analysis time
- Reliable Results: Automated curve fitting and EC₅₀ calculation ensure accurate biomarker quantification for drug screening
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Product family
Microplate Reader
Product primary application
SpectraMax iD3 and iD5 Multi-Mode Microplate Readers; Fluorescence Polarization (FP); Cell Health: Viability, Proliferation, Cytotoxicity, and Cellular Function
CMP
701Rn00000l3eibIAA
Title
High-Throughput hALT Assay for 3D Liver Microtissues
Resource URL
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