Organoid Innovation Center
Modular, automated 3D cell culture and image analysis lab enables customers to streamline and scale organoid interrogation, advancing drug discovery research
Automated 3D cell culture and image analysis lab streamlines and scales complex biology research
The new Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel 3D biology methods to address key challenges of scaling complex 3D biology.
The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house scientists.
An end-to-end solution standardizes the organoid development process with cell culture, treatment, and incubation, through to imaging, analysis, and data processing, delivering consistent, unbiased, and biologically-relevant results at scale.
Quickly adopt innovative, 3D biological methods and technologies for drug discovery
The center expands beyond imaging to demonstrate a fully-integrated solution that addresses the challenges associated with every step in the sample prep-to-report pipeline for assays performed on complex 3D biological models.
The Organoid Innovation Center showcases cutting-edge instruments that work harmoniously together for autonomous, long-term, live cell 2D and 3D cell culture growth and monitoring with intelligent label-free imaging. This integrated workflow provides quality control alerts and readiness, 3D organoid screening, and deep learning image analysis that reveals hidden patterns other technologies miss.
See the power and flexibility of an automated and customizable high-throughput screening solution
With intuitive scheduling software researchers can control the 3D workflow remotely, tracking the cell journey from single cell to differentiated organoid, along the way. Cell culture and incubation is streamlined with an automated incubator and collaborative robot that maintains culture consistency. Media exchange for culture maintenance is standardized and streamlined with automated liquid handling, minimizing manual intervention. 3D model development can be monitored over time with label-free imaging to assess assay readiness – and with real-time feedback, scheduling of automated compound addition and treatment is standardized.
Organoid Screening Workflow
- Step 1) 2d Pre-culture – Organoids are pre-cultured from iPSC-derived or primary cells (intestine, lung, or brain)
- Step 2) Developing 3D Organoids – Cells are transferred to 24-well plates then placed in an incubator to promote cell growth and differentiation into specific tissue in 3D
- Step 3) Organoid Culture – Organoid culture process require multiple steps with different media exchanges
- Step 4) Monitor organoid growth & development – Organoid are monitored and characterized for complex analysis of tissue structure and differentiation
- Step 5) Confocal imaging and 3D analysis – The visualization and characterization of multiple quantitative descriptors used for studying disease phenotypes and compound effects
Our new ImageXpress Confocal HT.ai High-Content Imaging system is designed for 3D imaging. This system offers eight high-powered laser excitation channels and automated water immersion objectives that boost signal and assay sensitivity without sacrificing speed. Spinning disk confocal technology with five pinhole geometry options reduces haze from out-of-focus light for deeper organoid penetration and improved axial resolution. For analysis of complex 3D biology, IN Carta Image Analysis Software provides a streamlined workflow with powerful deep-learning-based segmentation, machine learning-based classification, and 3D volumetric analysis.
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Complete solutions for a fully integrated lab automation workflow
Our lab automation solutions include scientists and engineers who can customize our instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. From incubators, liquid handlers, and robotics to customized software and hardware—and with over 35 years of experience in the life science industry—you can count on us to deliver quality products and provide worldwide support.
Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms
SLAS 2023 snapshot: Innovations, learnings, and what you may have missed
The Society for Lab Automation and Screening (SLAS) 2023 conference was another exciting year to learn about innovative laboratory technologies. Whether you attended in person or visited us online at our virtual events page, we were excited to share new methods and protocols to automate your complex biology workflows.
We caught up with Laura Dranschak, Director of Commercial Operations & Custom Solutions, to chat on all things SLAS 2023. See what all the buzz was about on the show floor, what the automation industry holds for labs of the future, and download our latest automation research poster on 3D complex assays.
Lung organoid cell image gallery
Image analysis of organoid
Lung organoids 2
Automated imaging of live organoids in Matrigel was done using confocal option, 4x or 10x magnification
High complexity lung organoid
Gut organoid dTomato cells in red Detection of Double positive(mNeon) in green
Internally expressed genes dTomato magenta mNeon green 10x
Intestinal organoid 40x,dTomato cell count
Lung organoid 10x 20x Multiple stains Hoechst MitoTracker
Lung organoids,projection image and a panel with Z stack; live stained with Hoechstblue,nuclei),MitoTracker(red,mitochondria),and Calcein AM(green,viability dye);20x water immersion
Lung organoids 3
Lung organoids 4
Lung organoids lung airway epithelial cells cultured in matrigel domes for 8 weeks
Organoid analysis multiplexed cell scoring
Organoid analysis multiplexed cell scoring 2
Applications and assays
The Organoid Innovation Center builds on Molecular Devices 35 years of experience delivering high-performance life science technology to customers for improved drug development, biotechnology research, and clone screening workflows.
Learn more about our industry leading 3D biology and automated high-content imaging applications: