Your All Access Pass to Unleash Your Brilliance
The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5 can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD5 is your all access pass to helping you unleash your brilliance.
Using built-in near-field communication (NFC) tags, the SpectraMax iD5 plate reader automatically detects the identification code of the filter to recognize the slide and filter configuration, eliminating confusion and simplifying your filter workflow.
Protein detection is an important task for pharmaceutical and clinical research, and Western Blots are one of the most common methods employed for this purpose. The SpectraMax iD5 plate reader, utilizing an optional enhanced TRF detection module, is capable of scanning and analyzing your membranes for western blot data. The Europium-incubated membranes are resistant to photo bleaching and allow you to read the membranes without loss of signal for longer periods.
Detect assay signal the best way using the hybrid monochromator- and filter-based optical system.
The innovative SpectraMax iD5 is the first five mode microplate reader featuring near-field communication (NFC) technology for touchscreen personalization, user acess control, and filter identification.
Simple-to-use temperature control allows you to adjust your experiment’s conditions from ambient up to 66°C, expanding your laboratory’s capabilities to include temperature sensitive assays.
The SpectraMax iD5 plate reader is injector-ready allowing you to expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 μL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.
SoftMax® Pro Software is the most published microplate reader control and microplate data analysis software package available to scientists. The inclusive package of ready-to-run protocols, analysis algorithms, and 21 different curve fit options provides the full solution from data acquisition to analysis to publishing. The FDA 21 CFR Part 11 compliance tools available in SoftMax Pro GxP Software for regulated laboratories deliver a complete solution to minimize validation costs, provide end-to-end chain of custody, and eliminate the need to validate additional analysis software.
The StakMax® Microplate Handling System is a compact benchtop microplate stacker that works with Molecular Devices SpectraMax® Microplate Readers and Microplate Washers to give you walk-away automation for up to 50 microplates. Use just one operating interface — SoftMax® Pro Software — to set up and run your whole workflow.
The SpectraDrop™ Micro-Volume Microplate handles volumes down to 2 µL and integrates seamlessly with the StakMax Microplate Stacker, enabling you to acquire more data, from smaller samples, in less time.
Standard read times (minutes:seconds)
Absorbance photometric performance
Fluorescence intensity performance
Optimized Top sensitivity (fluorescein)
Optimized Bottom sensitivity (fluorescein)
Fluorescence polarization performance
Time-Resolved Fluorescence performance
Injector system with SmartInject Technology (optional)
CV ≤ 2% at 100 μL
The SpectraMax iD5 is a hybrid monochromator- and filter-based, five-mode microplate reader that allows you to expand the boundaries of your research to include a wide variety of applications.
Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5 reader can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD5 reader is your all access pass to helping you unleash your brilliance.
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Traditional ELISA methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance analytes, such as inflammatory cytokines. The Sword ELISA Booster, a next-generation ELISA detection technology that directly replaces the detection reagents in a traditional, gold-standard ELISA, increases sensitivity up to 30 fold.
This enhanced assay allows you to detect lower levels of these cytokines and advance your understanding of diseases associated with these mediators of inflammation. Here, we demonstrate how three SpectraMax® microplate readers we tested met the criteria for sensitivity outlined for the Sword ELISA Boosters.
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Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most common reporter genes, as they are easily detectable using luminometers or luminescence microplate readers, and the low luminescent background present in cells enables high assay sensitivity. Firefly luciferase is typically used as a readout for expression of a gene of interest. Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter to normalize for transfection efficiency, cell viability, and other factors that may vary among assay samples. Here we show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. NF-κB is a ‘master regulator’ of gene expression for many cellular processes including inflammation, immunity, differentiation, proliferation, and apoptosis.
The process of cell line development for the production of antibody-based therapeutics can vary widely amongst researchers. The ultimate goal is to find a clone that stably produces large quantities of a high-quality monoclonal antibody. In this application note, we introduce the use of the Valita®TITER assay from Valitacell for measuring IgG titers during the antibody development and manufacturing process. Assay detection can be performed on multi-mode microplate readers from Molecular Devices: SpectraMax® iD5 Multi-Mode Microplate Reader, SpectraMax® M5 Multi-Mode Microplate Reader, and SpectraMax® i3x Multi-Mode Microplate Reader with the Fluorescence Polarization Detection Cartridge.
Getting to know your new SpectraMax iD5 Multi-Mode Microplate Reader
View a brief overview of the features and benefits of your new SpectraMax iD5 Multi-Mode Microplate Reader.
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Personalizing your new SpectraMax iD5 Multi-Mode Microplate Reader
Learn how to personalize your SpectraMax iD5 Multi-Mode Microplate Reader. Create user profiles and pair them to NFC tags to easily pull up saved protocols, and experiment results.
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Starting a plate read on your new SpectraMax iD5 Multi-Mode Microplate Reader
Learn how to quickly and easily start a plate read using the high-resolution touchscreen interface.
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Viewing results on your new SpectraMax iD5 Multi-Mode Microplate Reader
Tutorial on how to quickly and easily view your results on the SpectraMax iD5 Multi-Mode Microplate Reader.
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