ScanLater Western Blot Assay Kit
The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. Using this novel western blot method, researchers can:
- Time-resolved fluorescence detection
- Europium-labeled goat anti-rabbit, goat anti-mouse, donkey anti-rat, donkey anti-goat or streptavidin labeled antibodies
- Supports nitrocellulose and PVDF membranes
- 340/80 nm excitation with 616/10 nm emission range
- Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers
- Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate Reader
- Eliminate time-dependent substrate addition steps
- Sustain blot signal stability for at least one month
- Enhance assay sensitivity using TRF method to reduce background noise
- Maintain femtogram to picogram protein sensitivity similar to traditional western blot detector
Latest Resources
Data of ScanLater Western Blot Assay Kit
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Signal Stability over Time
Results demonstrate the signal stability of the ScanLater Assay System. Three dilutions of Transferrin were separated by electrophoresis and transferred to an Immobilon FL membrane and probed with rabbit anti-transferrin. Blots were incubated with the ScanLater Eu-labeled Anti-rabbit Secondary Antibodies. Membranes were measured on day 1 and day 30. Results represent a three-fold serial dilution of transferrin 1x sample buffer was loaded on a 4-20% gradient gel run for 30 min. Proteins were transferred to an Immobilon FL membrane and probed with rabbit anti-transferrin overnight at 4˚C followed by incubation with europium-labeled anti-rabbit IgG for 1 hr. Blots were washed, dried, and scanned on the same day and after a month using a SpectraMax Paradigm Reader.
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Signal Stability Across Multiple Scans
Results demonstrate the signal stability of the ScanLater Eu-labeled Secondary Antibodies after 7 successive measurements of the same membrane. Demonstrates stability of europium-labeled anti-rabbit IgG blots after 7 scans on the SpectraMax Paradigm Reader.
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Detection of Trace Amounts of Endogenous Phosphorylated Form of pChk1
Detection of phosphorylated form of pChk1 in HEK 293 cells on treatment with carcinogen MMS & UV radiation (Stanford University).
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Detection of Trace Amounts of Endogenous Phosphorylated Form of JNK1
Detection of phosphorylated form of JNK1 in NHLF (natural human lung fibroblast) cells treated with stimulants & inhibitory drugs.
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Comparison of ScanLater Western Blot System to Chemiluminescent Western Blot Detection
Detection of ubiquitinated Rad-18 in HEK 293 cells on treatment with different concentration (0, 50, 100 ppm) of carcinogen MMS, an alkylating agent. Comparable result using ScanLater Western Blot System vs. chemiluminescence western blot (Stanford University).
Technology of ScanLater Western Blot Assay Kit
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Emission Lifetimes of Background Fluorescence
Schematic representation of emission lifetimes of background fluorescence and Eu-chelate showing principle of TRF measurement. Signal is measured after appropriate time-delay to reduce auto-fluorescence.
Europium-Chelate Excitation and Emission Spectra
Schematic representation of Europium-chelate excitation and emission spectra
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ScanLater Western Blot Assay Principle
Schematic of protein detection method shows the secondary antibody is directly labeled with Europium.
Resources of ScanLater Western Blot Assay Kit
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
Scientific Poster
Western Blot Protein Detection and Quantitation Based on Europium Labeled Proteins Using a Plate Reader
Western Blot Protein Detection and Quantitation Based on Europium Labeled Proteins Using a Plate Reader
Explore PDF to know more about new SpectraMax Western Blot system, a simple, sensitive, & stable platform that provides excellent Western Blot capability in a microplate reader.…
Application Note
Estimation of Protein Molecular Weights with ScanLater Western Blot Protein Ladder
Estimation of Protein Molecular Weights with ScanLater Western Blot Protein Ladder
The ScanLater Western Blot Protein Ladder is an essential component of the ScanLater Western Blot Detection System. The primary applications of this protein ladder include the…
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Application Note
Detection and quantitation of protein with ScanLater Western Blot Detection System
Detection and quantitation of protein with ScanLater Western Blot Detection System
Protein detection is important for pharmaceutical and clinical research today, and western blots are among the most common methods employed for this purpose. Various techniques are…
Data Sheet
ScanLater Western Blot Detection System
ScanLater Western Blot Detection System
Read more about ScanLater Western Blot Detection System that includes ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Assay Kit, & image acquisition.
Compatible Products & Services of ScanLater Western Blot Assay Kit
Featured Applications
Emission Lifetimes of Background Fluorescence
Schematic representation of emission lifetimes of background fluorescence and Eu-chelate showing…
Europium-Chelate Excitation and Emission Spectra
Schematic representation of Europium-chelate excitation and emission spectra
ScanLater Western Blot Assay Principle
Schematic of protein detection method shows the secondary antibody is directly labeled with…
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