ScanLater Western Blot Assay Kit

The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. Using this novel western blot method, researchers can:

  • Time-resolved fluorescence detection
  • Europium-labeled goat anti-rabbit, goat anti-mouse, donkey anti-rat, donkey anti-goat or streptavidin labeled antibodies
  • Supports nitrocellulose and PVDF membranes
  • 340/80 nm excitation with 616/10 nm emission range
  • Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3 and Paradigm® Multi-Mode Platforms
  • Eliminate time-dependent substrate addition steps
  • Sustain blot signal stability for at least one month
  • Enhance assay sensitivity using TRF method to reduce background noise
  • Maintain femtogram to picogram protein sensitivity similar to traditional western blot detector

Data of ScanLater Western Blot Assay Kit

  • Signal stability over time

    Signal Stability over Time

    Results demonstrate the signal stability of the ScanLater Assay System. Three dilutions of Transferrin were separated by electrophoresis and transferred to an Immobilon FL membrane and probed with rabbit anti-transferrin. Blots were incubated with the ScanLater Eu-labeled Anti-rabbit Secondary Antibodies. Membranes were measured on day 1 and day 30. Results represent a three-fold serial dilution of transferrin 1x sample buffer was loaded on a 4-20% gradient gel run for 30 min. Proteins were transferred to an Immobilon FL membrane and probed with rabbit anti-transferrin overnight at 4˚C followed by incubation with europium-labeled anti-rabbit IgG for 1 hr. Blots were washed, dried, and scanned on the same day and after a month using a SpectraMax Paradigm Reader.

  • Signal Stability Across Multiple Scans

    Results demonstrate the signal stability of the ScanLater Eu-labeled Secondary Antibodies after 7 successive measurements of the same membrane. Demonstrates stability of europium-labeled anti-rabbit IgG blots after 7 scans on the SpectraMax Paradigm Reader.

    Signal stability across mulitple scans

  • Detection of Trace Amounts of Endogenous Phosphorylated Form of pChk1

    Detection of phosphorylated form of pChk1 in HEK 293 cells on treatment with carcinogen MMS & UV radiation (Stanford University).

  • Detection of Trace Amounts of Endogenous Phosphorylated Form of JNK1

    Detection of phosphorylated form of JNK1 in NHLF (natural human lung fibroblast) cells treated with stimulants & inhibitory drugs.

    Detection of trace amounts of endogenous phosphorylated form of JNK1

  • Comparison of ScanLater Western Blot System to chemiluminescent western blot detection

    Comparison of ScanLater Western Blot System to Chemiluminescent Western Blot Detection

    Detection of ubiquitinated Rad-18 in HEK 293 cells on treatment with different concentration (0, 50, 100 ppm) of carcinogen MMS, an alkylating agent. Comparable result using ScanLater Western Blot System vs. chemiluminescence western blot (Stanford University).

Technology of ScanLater Western Blot Assay Kit

  • Workflow of ScanLater Western Blot Assay

    ScanLater Western Blot Assay Workflow

  • Principle of ScanLater Western Blot Assay

    ScanLater Western Blot Assay Principle

    Schematic of protein detection method shows the secondary antibody is directly labeled with Europium.

  • Europium-Chelate Excitation and Emission Spectra

    Europium-Chelate Excitation and Emission Spectra

    Schematic representation of Europium-chelate excitation and emission spectra

  • Emission Lifetimes of Background Fluorescence

    Emission Lifetimes of Background Fluorescence

    Schematic representation of emission lifetimes of background fluorescence and Eu-chelate showing principle of TRF measurement. Signal is measured after appropriate time-delay to reduce auto-fluorescence.

Ordering Options of ScanLater Western Blot Assay Kit

ScanLater: Eu-Streptavidin Kit

Evaluation Kit

  • Europium-labeled streptavidin (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol

#R8200

$309.00 USD

1

Bulk Kit

  • Europium-labeled streptavidin (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol

#R8203

$660.00 USD

1

ScanLater: Anti-Mouse Kit

Evaluation Kit

  • Europium-labeled goat anti-mouse antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol

#R8201

$309.00 USD

1

Bulk Kit

  • Europium-labeled goat anti-mouse antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol

#R8205

$660.00 USD

1

ScanLater: Anti-Rabbit Kit

Evaluation Kit

  • Europium-labeled goat anti-rabbit antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol

#R8202

$309.00 USD

1

Bulk Kit

  • Europium-labeled goat anti-rabbit antibody (60 μg)
  • Blocking buffer (120 mL), Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol

#R8204

$660.00 USD

1

ScanLater: Anti-Goat Kit

Evaluation Kit

  • Europium-labeled donkey anti-goat antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol

#R8224

$299.00 USD

1

Bulk Kit

  • Europium-labeled donkey anti-goat antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol

#R8227

$629.00 USD

1

ScanLater: Anti-Rat Kit

Evaluation Kit

  • Europium-labeled donkey anti-rat antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol

#R8225

$299.00 USD

1

Bulk Kit

  • Europium-labeled donkey anti-rat antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol

#R8228

$629.00 USD

1

ScanLater Western Blot Protein Ladder

Protein Ladder

  • 7 biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD
  • 3 pre-stained marker proteins at 18, 31, and 70 kD
  • Ready to use, for 30 mini gels with recommended protocol

#R8220

$299.00 USD

1

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.