SpectraMax Paradigm Multi-Mode Microplate Reader

AlphaScreen

AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular binding. Donor and acceptor beads are attached to biomolecules, and when the beads are brought into proximity (via binding), a cascade of chemical reactions occurs, amplifying a signal that is detected on the SpectraMax® i3x and Paradigm® Multi-Mode Microplate Readers.

HTRF

HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

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Kinase/Phosphatase Assays

Kinases are one of the most critical targets in drug discovery today. These enzymes are key components in cellular signaling pathways, and disruption to their function causes a variety of diseases, such as metabolic diseases, certain cancers, and cardiac disease. The functionally related phosphatases and phosphodiesterases are also important screening targets.

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Western Blot Detection

Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. As well as an introduction to a novel, first-of-its-kind western blot detection in a multi-mode microplate reader platform.

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