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SpectraMax Paradigm Multi-Mode Microplate Reader
High-throughput screening capability on one fast, configurable microplate reader
Z-height and microplate optimization increases assay dynamic range and sensitivity
The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Detection cartridge modularity allows for easy addition of new read modes within minutes without requiring a service visit, while providing the flexibility for future applications.
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Get automatic Z-height optimization
Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
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Eliminate manual gain adjustment
The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.
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Tailor your data analysis
Perform multiple calculations on one data set, compare multiple experiments side-by-side, and adjust reduction parameters while collecting real-time data with SoftMax® Pro Software.

SpectraMax Paradigm Multi-Mode Microplate Reader
Features
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Configuration changes in minutes
Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.
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Increased dynamic range and sensitivity
Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.
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Application Note
GeneBLAzer cell-based FRET Assay for G-protein coupled receptors
GeneBLAzer cell-based FRET Assay for G-protein coupled receptors
GeneBLAzer assay is a fluorescence resonance energy transfer (FRET)-based method to study G-protein-coupled receptors and other drug targets in vivo (living cells) or in vitro (cell lysates…
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Application Note
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
In this application note we show how the SpectraMax® Paradigm® Multi-Mode Microplate Reader is used to perform robust, no-wash cytokine assays with excellent Z’ factors. HTRF® is a…
Applications of SpectraMax Paradigm Multi-Mode Microplate Reader
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AlphaScreen
AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular binding. Donor and acceptor beads are attached to biomolecules, and when the beads are brought into proximity (via binding), a cascade of chemical reactions occurs, amplifying a signal that is detected on the SpectraMax® i3x and Paradigm® Multi-Mode Microplate Readers.
AlphaScreen
AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular binding. Donor and acceptor beads are attached to biomolecules, and when the beads are brought into proximity (via binding), a cascade of chemical reactions occurs, amplifying a signal that is detected on the SpectraMax® i3x and Paradigm® Multi-Mode Microplate Readers.
HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.) -
Kinase/Phosphatase Assays
Kinases are one of the most critical targets in drug discovery today. These enzymes are key components in cellular signaling pathways, and disruption to their function causes a variety of diseases, such as metabolic diseases, certain cancers, and cardiac disease. The functionally related phosphatases and phosphodiesterases are also important screening targets.
Kinase/Phosphatase Assays
Kinases are one of the most critical targets in drug discovery today. These enzymes are key components in cellular signaling pathways, and disruption to their function causes a variety of diseases, such as metabolic diseases, certain cancers, and cardiac disease. The functionally related phosphatases and phosphodiesterases are also important screening targets.
Western Blot Detection
Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. As well as an introduction to a novel, first-of-its-kind western blot detection in a multi-mode microplate reader platform.
Western Blot Detection
Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. As well as an introduction to a novel, first-of-its-kind western blot detection in a multi-mode microplate reader platform.
Specifications & Options of SpectraMax Paradigm Multi-Mode Microplate Reader
Resources of SpectraMax Paradigm Multi-Mode Microplate Reader
Application Note
GeneBLAzer cell-based FRET Assay for G-protein coupled receptors
GeneBLAzer cell-based FRET Assay for G-protein coupled receptors
GeneBLAzer assay is a fluorescence resonance energy transfer (FRET)-based method to study G-protein-coupled receptors and other drug targets in vivo (living cells) or in vitro (cell…
Application Note
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
In this application note we show how the SpectraMax® Paradigm® Multi-Mode Microplate Reader is used to perform robust, no-wash cytokine assays with excellent Z’ factors. HTRF® is a…
Application Note
AlphaLISA Screen on the SpectraMax Paradigm Multi-Mode Microplate Detection Platform
AlphaLISA Screen on the SpectraMax Paradigm Multi-Mode Microplate Detection Platform
Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine production and adhesion molecule expression, which may…
Application Note
Maximizing performance of the SpectraDrop Micro-Volume Microplate for DNA quantitation
Maximizing performance of the SpectraDrop Micro-Volume Microplate for DNA quantitation
Molecular Devices SpectraDrop™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax® Microplate Readers, with sample volumes as low as 2 µL. The…
Application Note
Fluorescence-based quantitation of small DNA samples with the SpectraDrop Micro-Volume Microplate
Fluorescence-based quantitation of small DNA samples with the SpectraDrop Micro-Volume Microplate
Formerly known as the μMax, Molecular Devices’ unique SpectraDrop™ Micro- Volume Microplate offers the highest throughput solution for low volume measurement available on the market…
Application Note
Optimized Wavelength Scanning of Fluorescent Proteins with the SpectraMax Paradigm Platform and TUNE Technology
Optimized Wavelength Scanning of Fluorescent Proteins with the SpectraMax Paradigm Platform and TUNE Technology
The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with…
Application Note
Migration Assay for High Throughput Screening ( HTS )
Migration Assay for High Throughput Screening ( HTS )
Cell migration is a critical in the development and maintenance of multicellular organisms. Tissue formation during embryonic development, wound healing and immune responses all…
Application Note
IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers
IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers
Cyclic nucleotide phosphodiesterases (PDEs) are a group of enzymes that degrade the phosphodiester bond of cAMP and cGMP, second messengers that are involved in a variety of biological…
Application Note
HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
HTRF is a versatile technology developed by Cisbio Bioassays for detecting biomolecular interactions1. It combines fluorescence resonance energy transfer (FRET) technology with time-…
Application Note
Detection and quantitation of protein with ScanLater Western Blot Detection System
Detection and quantitation of protein with ScanLater Western Blot Detection System
Protein detection is important for pharmaceutical and clinical research today, and western blots are among the most common methods employed for this purpose. Various techniques are…
Data Sheet
Multi-Mode Validation Plate
Multi-Mode Validation Plate
Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader.
Data Sheet
Tunable Wavelength SpectraMax Detection Cartridge
Tunable Wavelength SpectraMax Detection Cartridge
Read more about TUNE SpectraMax Detection Cartridges for the SpectraMax Paradigm System that offers unmatched sensitivity in wavelength tunable detection.
Data Sheet
SpectraMax Paradigm Time-Resolved Fluorescence Detection Cartridge
SpectraMax Paradigm Time-Resolved Fluorescence Detection Cartridge
Explore SpectraMax Paradigm, a time-resolved fluorescence detection cartridge built to measure Europium & Samarium & is ideal for protein-protein interaction, GPCR & enzyme…
Data Sheet
SpectraMax Paradigm Multi-Mode Detection Cartridge
SpectraMax Paradigm Multi-Mode Detection Cartridge
Read PDF & explore more about SpectraMax Paradigm Multi-Mode Detection Cartridge that provides a broad array of measurement capabilities like FI, TRF & LUM.
Data Sheet
Dual Color Luminescence, SpectraMax Detection Cartridge
Dual Color Luminescence, SpectraMax Detection Cartridge
Explore Dual Color Luminescence Detection Cartridges designed to provide optimal performance when used with BRET2 reagents on SpectraMax Paradigm, i3 or i3x.
Data Sheet
Luminescence SpectraMax Detection Cartridge
Luminescence SpectraMax Detection Cartridge
Explore PDF of Luminescence SpectraMax Detection Cartridge for spectra may i3 & Paradigm systems that utilize a patent pending design to provide optimal performance for glow lumines…
Data Sheet
HTRF SpectraMax Detection Cartridge
HTRF SpectraMax Detection Cartridge
Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents.
Data Sheet
Fluorescence Intensity GeneBLAzer SpectraMax Detection Cartridge
Fluorescence Intensity GeneBLAzer SpectraMax Detection Cartridge
Explore Fluorescence Intensity GeneBLAzer SpectraMax Detection Cartridge for SpectraMax i3x & Paradigm Systems.
Data Sheet
Fluorescence Polarization SpectraMax Detection Cartridge
Fluorescence Polarization SpectraMax Detection Cartridge
Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems.
Data Sheet
Fluorescence Intensity SpectraMax Detection Cartridge
Fluorescence Intensity SpectraMax Detection Cartridge
Explore this datasheet to know more about Fluorescence Intensity Detection Cartridges for SpectraMax Paradigm and i3x, which provide speed, sensitivity, and flexibility & uncompromi…
Data Sheet
SpectraMax Paradigm AlphaScreen Detection Cartridges
SpectraMax Paradigm AlphaScreen Detection Cartridges
Download this PDF to explore SpectraMax Paradigm AlphaScreen Detection Cartridges which utilize a 680 nm laser diode to provide a sensitive reading system for AlphaScreen assays.
Data Sheet
Absorbance SpectraMax Detection Cartridge
Absorbance SpectraMax Detection Cartridge
Explore use of Absorbance SpectraMax Detection Cartridges for SpectraMax Paradigm System. Combine scanning & find a broad spectrum wavelength range necessary to address a variety of…
Data Sheet
SpectraMax Paradigm Microplate Reader
SpectraMax Paradigm Microplate Reader
Explore SpectraMax Paradigm Microplate Reader, a user-upgradeable high-throughput multi-mode detection platform where a broad array of Paradigm Detection Cartridges enable the user to p…
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
Brochure
SoftMax Pro 6 Microplate Data Acquisition and Analysis Software
SoftMax Pro 6 Microplate Data Acquisition and Analysis Software
Learn how the SoftMax Pro Software, which offers comprehensive data capture, reduction, and analysis capabilities for Molecular Device's microplate readers.
Brochure
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. Our…
Scientific Poster
Multiplexed High Content Assays for Predictive Hepatotoxicity using Induced Pluripotent stem Cell Derived Hepatocytes
Multiplexed High Content Assays for Predictive Hepatotoxicity using Induced Pluripotent stem Cell Derived Hepatocytes
A large percentage of drugs fail in clinical studies,or are withdrawn from the market due to hepatic toxicity. Therefore, highly predictive in vitro assays suitable for safety and effic…
Scientific Poster
Homogeneous High Throughput Live Cell GPCR Functional and Surface Binding Assays
Homogeneous High Throughput Live Cell GPCR Functional and Surface Binding Assays
Explore the use of tag lite assay platform with SpectraMax Paradigm plate reader for characterization of GPCR agonist & antagonists in Tag-lite receptor ligand binding assays.
Scientific Poster
High‐Throughput Homogeneous Epigenetics Assays Using HTRF Technology and the SpectraMax Paradigm Microplate Detection Platform
High‐Throughput Homogeneous Epigenetics Assays Using HTRF Technology and the SpectraMax Paradigm Microplate Detection Platform
PDF explores the optimization of high-throughput homogeneous epigenetics assays by using HTRF technology on SpectraMax Paradigm microplate detection platform.
Scientific Poster
High Throughput Cell Based Assays for Toxicity Assessment using SpectraMax® Paradigm Plate Reader
High Throughput Cell Based Assays for Toxicity Assessment using SpectraMax® Paradigm Plate Reader
Application note presents a comparison of assay performance between SpectraMax Paradigm reader read types, and the ImageXpress Micro imaging system.
Videos and Demos

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery

SpectraMax Paradigm Multi-Mode Microplate Reader
SpectraMax® Paradigm Multi-Mode Microplate Reader
Product |
Part Number |
SpectraMax Paradigm Multi-Mode Microplate Reader with SoftMax Pro Software | PARADIGM |
SpectraMax Absorbance (ABS) Detection Cartridge
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0200-7000
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SpectraMax Tunable Wavelength (TUNE) Detection Cartridge
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0200-7050
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SpectraMax Multi-mode (MULTI) Detection Cartridge
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0200-7001
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SpectraMax Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
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0200-7002
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SpectraMax Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
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0200-7003
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SpectraMax Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
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0200-7004
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SpectraMax Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
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0200-7005
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SpectraMax Fluorescence Intensity (FI) GeneBLAzer Detection Cartridge
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0200-7006
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SpectraMax Time Resolved Fluorescence (TRF) Detection Cartridge
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0200-7008
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SpectraMax Fluorescence Polarization (FP) (fluorescein) Detection Cartridge
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0200-7009
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SpectraMax Fluorescence Polarization (FP) (rhodamine) Detection Cartridge
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0200-7010
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SpectraMax Cisbio HTRF Detection Cartridge
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0200-7011
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SpectraMax Glow Luminescence (LUM) Detection Cartridge
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0200-7012
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SpectraMax Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge
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0200-7013
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SpectraMax Glow Luminescence (LUM) (96) Detection Cartridge
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0200-7014
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SpectraMax Glow Luminescence (LUM) (384) Detection Cartridge
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0200-7015
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SpectraMax Dual Color Luminescence (LUM) (BRET2TM) Detection Cartridge
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0200-7016
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SpectraMax AlphaScreen 384 STD Detection Cartridge
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0200-7017
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SpectraMax AlphaScreen 384 HTS Detection Cartridge
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0200-7018
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SpectraMax AlphaScreen 1536 HTS Detection Cartridge
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0200-7019
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Other Accessories |
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Product Name
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Part Number
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0200-6262
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0200-6267
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Multi-Mode Validation Plate for SpectraMax® Paradigm® & FilterMax™ F3/F5 Systems
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0200-7200
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Related Products & Services of SpectraMax Paradigm Multi-Mode Microplate Reader
Featured Applications

AlphaScreen
AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular…

HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance…

Kinase/Phosphatase Assays
Kinases are one of the most critical targets in drug discovery today. These enzymes are key…

Western Blot Detection
Western blotting is a popular technique used for protein detection and quantitation. Learn about…
Customer Breakthrough


SUCCESS STORY
University of Copenhagen uses the SpectraMax Paradigm reader to investigate cancer metastasis
Understanding how cancer spreads through the body (a process called metastasis) as this is responsible for over 90% of cancer patient deaths.
Ready to get started?
We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.
Maximize flexibility in application workflow and experimental design
Contact our specialist today to discuss how to implement a solution that works for you
Ready to get started?
We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.
Maximize flexibility in application workflow and experimental design
Contact our specialist today to discuss how to implement a solution that works for you