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Z-height and microplate optimization increases assay dynamic range and sensitivity

The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Detection cartridge modularity allows for easy addition of new read modes within minutes without requiring a service visit, while providing the flexibility for future applications.

  • Get automatic Z-height optimization

    Get automatic Z-height optimization

    Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.

  • Eliminate manual gain adjustment

    Eliminate manual gain adjustment

    The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.

  • Tailor your data analysis

    Tailor your data analysis

    Perform multiple calculations on one data set, compare multiple experiments side-by-side, and adjust reduction parameters while collecting real-time data with SoftMax® Pro Software.

SpectraMax Paradigm Multi-Mode Microplate Reader

SpectraMax Paradigm Multi-Mode Microplate Reader

Features

  • Configuration changes in minutes

    Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.

  • Increased dynamic range and sensitivity

    Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.

Applications of SpectraMax Paradigm Multi-Mode Microplate Reader

  • Absorbance

    Absorbance

    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    AlphaScreen

    AlphaScreen Technology

    AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular binding. Donor and acceptor beads are attached to biomolecules, and when the beads are brought into proximity (via binding), a cascade of chemical reactions occurs, amplifying a signal that is detected on the SpectraMax® i3x and Paradigm® Multi-Mode Microplate Readers.

    Read Application Note 

  • ELISA

    Elisa

    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

    Fluorescence

    Fluorescence

    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  

  • Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

    Learn more  

    HTRF

    HTRF

    HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
    (This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

    Read Application Note 

  • Kinase/Phosphatase Assays

    Kinase/Phosphatase Assays

    Kinases are one of the most critical targets in drug discovery today. These enzymes are key components in cellular signaling pathways, and disruption to their function causes a variety of diseases, such as metabolic diseases, certain cancers, and cardiac disease. The functionally related phosphatases and phosphodiesterases are also important screening targets.

    Read Application Note 

    Luminescence

    Luminescence

    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

    Learn more  

  • TRF, TR-FRET & HTRF

    Time-resolved

    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

    Learn more  

    Western Blot

    Western Blot Detection

    Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. We’ll also introduce you to a novel, first-of-its-kind western blot detection system on a multi-mode microplate reader.

    Learn more 

Specifications & Options of SpectraMax Paradigm Multi-Mode Microplate Reader

 

*Using lowest settings and speed read when available.

Resources of SpectraMax Paradigm Multi-Mode Microplate Reader

Presentations
Videos & Webinars
Microplate Readers from ELISA to AlphaScreen

Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Pluripotent Stem Cell Applications in Drug Discovery

Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery

SpectraMax Paradigm Multi-Mode Microplate Reader

SpectraMax Paradigm Multi-Mode Microplate Reader

  • Citation
    Dated: Dec 15, 2020
    Publication Name: Molecular Devices

    High Throughput Cell Based Assays for Toxicity Assessment using SpectraMax® Paradigm Plate Reader

    Development of predictive in vitro assays suitable for safety and efficacy testing is of high interest for improving the drug development process and reducing drug attrition. Recently, there has been a great interest in using stem cells as tools for screening compounds during early drug development. Cardiomyocytes, neuronal progenitors,… View more

    Development of predictive in vitro assays suitable for safety and efficacy testing is of high interest for improving the drug development process and reducing drug attrition. Recently, there has been a great interest in using stem cells as tools for screening compounds during early drug development. Cardiomyocytes, neuronal progenitors, hepatocytes, and hematopoietic stem cells have all been shown to be attractive cell models for performing a variety of assays. Typically, such assays are performed using high content imaging and analysis (HCA) that allows individual assessment of cell response and multi-parametric outputs. However, there is a need for higher throughput methods with similar sensitivity. Here we present results obtained from the SpectraMax® Paradigm® Plate Reader for performing toxicity assessment of various compounds using stem cell derived cell models in a high throughput manner. The system is configured for cell-based assays with a highly sensitive direct bottom fluorescence read. Plates can be scanned using “Well Scan” or “On-The-Fly” read types. Several viability markers were evaluated including Calcein AM, actin, and mitochondrial stain. We have characterized a number of known hepatotoxic compounds and IC50 values were determined. Excellent agreement was found between plate reader and HCA results.

    Contributors: Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell  
    Go to article

  • Citation
    Dated: Aug 15, 2020
    Publication Name: Ecotoxicology and Environmental Safety

    Responses of GABA shunt coupled with carbon and nitrogen metabolism in poplar under NaCl and CdCl2 stresses

    The γ-aminobutyric acid (GABA) shunt is closely associated with plant tolerance; however, little is known about its mechanism. This study aimed to decipher the responses of the GABA shunt and related carbon–nitrogen metabolism in poplar seedlings (Populus alba × Populus glandulosa) treated with different NaCl and CdCl2 concentrations for 30 h. The… View more

    The γ-aminobutyric acid (GABA) shunt is closely associated with plant tolerance; however, little is known about its mechanism. This study aimed to decipher the responses of the GABA shunt and related carbon–nitrogen metabolism in poplar seedlings (Populus alba × Populus glandulosa) treated with different NaCl and CdCl2 concentrations for 30 h. The results showed that the activities of glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) were activated, as well as α-ketoglutarate dehydrogenase (α-KGDH) and succinate dehydrogenase (SDH) activities were enhanced by NaCl and CdCl2 stresses, except for SDH under CdCl2 stress. Meanwhile, the expression levels of GADs, GABA-TSDHs, succinyl-CoA ligases (SCSs), and succinic acid aldehyde dehydrogenases (SSADHs) were also increased. Notably, significant increases in the key components of GABA shunt, Glu and GABA, were observed under both stresses. Soluble sugars and free amino acids were enhanced, whereas citrate, malate and succinate were almost inhibited by both NaCl and CdCl2 stresses except that citrate was not changed or just increased by 50-mM NaCl stress. Thus, these results suggested that the carbon–nitrogen balance could be altered by activating the GABA shunt when main TCA-cycle intermediates were inhibited under NaCl and CdCl2 stresses. This study can enhance the understanding about the functions of the GABA shunt in woody plants under abiotic stresses and may be applied to the genetic improvement of trees for phytoremediation.

    Contributors: Jing Jia, Zheng Shi, Tiantian Xie, Xiaoman Zhang, Wei Chen, Changjian Du, Jiacheng Sun, Jianyun Yue, Xiulian Zhao, Zeping Jiang, Shengqing Shi  
    Go to article

  • Citation
    Dated: Mar 30, 2016
    Publication Name: Frontiers

    Efficacy and mechanism of selenium nanoparticles as antibacterial agents

    The rise in antibiotic resistance has become a daunting concern in the last decade, including strains like methicillin-resistant Staphylococcus aureus (MRSA), causing significant health problems and extensive medical bills. It is important, then, to explore novel, non-drug antibiotics to reduce these life-threatening infections. Selenium… View more

    The rise in antibiotic resistance has become a daunting concern in the last decade, including strains like methicillin-resistant Staphylococcus aureus (MRSA), causing significant health problems and extensive medical bills. It is important, then, to explore novel, non-drug antibiotics to reduce these life-threatening infections. Selenium nanoparticles (SeNP) have been shown to exhibit specific toxicity to bacteria while remaining non-toxic to healthy mammalian cells.

    Contributors: Michelle Stolzoff, Steve Q. Wang and Thomas J. Webster  
    Go to article

SpectraMax® Paradigm Multi-Mode Microplate Reader

Product

Part Number

SpectraMax Paradigm Multi-Mode Microplate Reader with SoftMax Pro Software PARADIGM
SpectraMax Absorbance (ABS) Detection Cartridge
0200-7000
SpectraMax Tunable Wavelength (TUNE) Detection Cartridge
0200-7050
SpectraMax Multi-mode (MULTI) Detection Cartridge
0200-7001
SpectraMax Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
0200-7002
SpectraMax Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
0200-7003
SpectraMax Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
0200-7004
SpectraMax Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
0200-7005
SpectraMax Fluorescence Intensity (FI) GeneBLAzer Detection Cartridge
0200-7006
SpectraMax Time Resolved Fluorescence (TRF) Detection Cartridge
0200-7008
SpectraMax Fluorescence Polarization (FP) (fluorescein) Detection Cartridge
0200-7009
SpectraMax Fluorescence Polarization (FP) (rhodamine) Detection Cartridge
0200-7010
SpectraMax Cisbio HTRF Detection Cartridge
0200-7011
SpectraMax Glow Luminescence (LUM) Detection Cartridge
0200-7012
SpectraMax Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge
0200-7013
SpectraMax  Glow Luminescence (LUM) (96) Detection Cartridge
0200-7014
SpectraMax Glow Luminescence (LUM) (384) Detection Cartridge
0200-7015
SpectraMax Dual Color Luminescence (LUM) (BRET2TM) Detection Cartridge
0200-7016
SpectraMax AlphaScreen 384 STD Detection Cartridge
0200-7017
SpectraMax AlphaScreen 384 HTS Detection Cartridge
0200-7018
SpectraMax AlphaScreen 1536 HTS Detection Cartridge
0200-7019
 

Other Accessories

 Product Name
Part Number
0200-6262
0200-6267
Multi-Mode Validation Plate for SpectraMax® Paradigm® & FilterMax™ F3/F5 Systems
0200-7200

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