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Protein Detection, Quantitation and Analysis

Streamline the process of protein quantitation

Detect, quantify, and analyze proteins

Protein detection, quantitation, and analysis is central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.

Cellular signaling and other biological processes may be analyzed using fluorescent proteins. For example green fluorescent protein (GFP) can be expressed in living cells and used to visualize the localization and protein dynamics under experimental conditions. Tryptophan, an amino acid whose fluorescence emission properties are affected by its microenvironment, has also been used to gain information on changes in the conformational state of proteins.

Protein quantitation methods and techniques

Microplate readers with absorbance, fluorescence, and luminescence detection modes provide a versatile solution for the quantitation and study of proteins. Our application notes demonstrate how these instruments can be used to obtain and analyze results.

Resources for Protein quantitation and analysis