Application Note

Monitoring receptor mediated changes in [Ca2+] i using single- and dual-wavelength indicators on the FlexStation 3 reader

  • Versatile reader for kinetic, cell-based fluorometric assays such as the measurement of intracellular calcium, membrane potential, and intracellular pH
  • Reliable, reproducible data for calcium mobilization assays used to monitor Gq-coupled GPCRs
  • Ratiometric properties of the dye result in a five-fold increase in signal