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Automated pipetting ensures consistent performance over multiple assay conditions on a microplate

The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics.

  • Enable more assay conditions

    Enable more assay conditions

    Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate.

  • Automation

    Automate pipetting

    Improve assay quality and increase throughput with automated pipetting ensuring consistent addition time and minimizing errors, thus providing tighter assay CVs within and between experiments.

  • Validate your reader

    Validate your reader

    Get fluid transfer validation with our PathCheck® Feature to quantify pipettor head performance. Use it with SoftMax® Pro Software and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance.

FlexStation 3 Multi-Mode Microplate Reader

FlexStation 3 Multi-Mode Microplate Reader


  • Onboard pipettor

    Integrated 8-channel or 16-channel pipettor increases assay flexibility by transferring reagents from 96 or 384 distinct wells in a source plate to the read plate. 

  • Optimal assay excitation

    Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.

  • Low volume applications

    SpectraDrop™ Micro-Volume Microplate offers a high-throughput solution for low volume measurement, accelerating sample preparation time and increased productivity in DNA, RNA and protein research.

  • Robot integration

    The FlexStation 3 reader can be integrated with all leading partner robots and with our out-of-the-box automation solution for savings on up-front integration time and resources.

Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational specifications
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs


Applications of FlexStation 3 Multi-Mode Microplate Reader

  • Absorbance


    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

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    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

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  • Enzyme Activity

    Enzyme Activity

    Enzyme activity can be monitored in real time using a chromogenic substrate which, upon its addition to the enzyme, produces a change in color that is detectable on an absorbance microplate reader. Using an instrument with onboard liquid handling capability, substrate can be added automatically while the reaction is monitored, enabling detection of the earliest part of the reaction and thus more accurate estimates of binding affinity.

    Read Application Note 

    Fast Kinetic Calcium Mobilization Assays

    Kinetic Calcium Mobilization Assays

    Gq protein-coupled receptor (GPCRs) activation can be monitored by measuring the increase in intracellular calcium with fluorescent dyes. A calcium-sensitive dye is taken into the cytoplasm of the cell. Upon ligand binding to the receptor, calcium is released into the cytoplasm of the cell. The dye binds to the intracellular calcium and an increase in fluorescence is measured.

    Read Application Note 

  • Fluorescence


    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

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    Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

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  • Luminescence


    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

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    Reporter Gene Assay

    SpectraMax DuoLuc Reporter Assay High Resolution

    Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.

    Read Application Note 



    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

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Specifications & Options of FlexStation 3 Multi-Mode Microplate Reader


*Using lowest settings and speed read when available.

Resources of FlexStation 3 Multi-Mode Microplate Reader

Videos & Webinars
Microplate Readers from ELISA to AlphaScreen

Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen

FlexStation 3 Multi-Mode Microplate Reader

FlexStation 3 Multi-Mode Microplate Reader

FlexStation® 3 Series Multi-Mode Microplate Readers

Product Part Number
FlexStation 3 Microplate Reader with SoftMax Pro Software Includes: FlexStation 3 Base System, 1 year warranty covering parts & labor. **Does not include 8 or 16 channel pipettor head, must be purchased separately and MUST be ordered at the time of ordering the instrument. Flex3
SpectraDrop™ Micro-Volume Starter Kit 0200-6262
SpectraDrop™ Micro-Volume HTS Kit 0200-6267
FlexStation 3 Benchtop Multi-Mode Microplate Reader IQ/OQ Validation Protocols 0112-0160

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