Automated pipetting ensures consistent performance over multiple assay conditions on a microplate
The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics.
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Enable more assay conditions
Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate.
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Automate pipetting
Improve assay quality and increase throughput with automated pipetting ensuring consistent addition time and minimizing errors, thus providing tighter assay CVs within and between experiments.
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Validate your reader
Get fluid transfer validation with our PathCheck® Feature to quantify pipettor head performance. Use it with SoftMax® Pro Software and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance.
FlexStation 3 Multi-Mode Microplate Reader
Features
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Onboard pipettor
Integrated 8-channel or 16-channel pipettor increases assay flexibility by transferring reagents from 96 or 384 distinct wells in a source plate to the read plate.
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Optimal assay excitation
Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.
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Low volume applications
SpectraDrop™ Micro-Volume Microplate offers a high-throughput solution for low volume measurement, accelerating sample preparation time and increased productivity in DNA, RNA and protein research.
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Robot integration
The FlexStation 3 reader can be integrated with all leading partner robots and with our out-of-the-box automation solution for savings on up-front integration time and resources.
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Application Note
Development of a platelet calcium flux assay using Fura-2, AM on the FlexStation 3 reader
Development of a platelet calcium flux assay using Fura-2, AM on the FlexStation 3 reader
Platelets are small, anucleate blood cells that mediate haemostasis by aggregating at sites of blood vessel injury to form a thrombus (or clot) that limits blood loss. When platelets…
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Application Note
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most…
Applications of FlexStation 3 Multi-Mode Microplate Reader
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Fast Kinetic Calcium Mobilization Assays
Gq protein-coupled receptor (GPCRs) activation can be monitored by measuring the increase in intracellular calcium with fluorescent dyes. A calcium-sensitive dye is taken into the cytoplasm of the cell. Upon ligand binding to the receptor, calcium is released into the cytoplasm of the cell. The dye binds to the intracellular calcium and an increase in fluorescence is measured.
Fast Kinetic Calcium Mobilization Assays
Gq protein-coupled receptor (GPCRs) activation can be monitored by measuring the increase in intracellular calcium with fluorescent dyes. A calcium-sensitive dye is taken into the cytoplasm of the cell. Upon ligand binding to the receptor, calcium is released into the cytoplasm of the cell. The dye binds to the intracellular calcium and an increase in fluorescence is measured.
Enzyme Activity
Enzyme activity can be monitored in real time using a chromogenic substrate which, upon its addition to the enzyme, produces a change in color that is detectable on an absorbance microplate reader. Using an instrument with onboard liquid handling capability, substrate can be added automatically while the reaction is monitored, enabling detection of the earliest part of the reaction and thus more accurate estimates of binding affinity.
Enzyme Activity
Enzyme activity can be monitored in real time using a chromogenic substrate which, upon its addition to the enzyme, produces a change in color that is detectable on an absorbance microplate reader. Using an instrument with onboard liquid handling capability, substrate can be added automatically while the reaction is monitored, enabling detection of the earliest part of the reaction and thus more accurate estimates of binding affinity.
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Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Specifications & Options of FlexStation 3 Multi-Mode Microplate Reader
Resources of FlexStation 3 Multi-Mode Microplate Reader
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Application Note
Development of a platelet calcium flux assay using Fura-2, AM on the FlexStation 3 reader
Development of a platelet calcium flux assay using Fura-2, AM on the FlexStation 3 reader
Platelets are small, anucleate blood cells that mediate haemostasis by aggregating at sites of blood vessel injury to form a thrombus (or clot) that limits blood loss. When platelets…
-
Application Note
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most…
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Application Note
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FlexStation 3 Multi-Mode Microplate Reader
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FlexStation 3 Multi-Mode Microplate Reader
We present the utility of a new potassium assay kit on the FLIPR® Tetra System to investigate hERG compound activity. The assay exploits the permeability of thallium (Tl+) for…
Data Sheet
FlexStation 3 Multi-Mode Microplate Reader
FlexStation 3 Multi-Mode Microplate Reader
This datasheet for the FlexStation 3 Multi-Mode Microplate Reader can be utilized in a variety of experiments using its five read-modes and flexible liquid transfer and pipetting capabi…
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Application Note
Calcium flux assay for in vitro neurotoxicity studies and drug screening
Calcium flux assay for in vitro neurotoxicity studies and drug screening
Calcium imaging, or monitoring changes in intracellular calcium, is an extremely useful technique for investigating the variety of roles that calcium ions have in functioning neurons.…
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Application Note
Detect dual luciferase expression on the FlexStation 3 microplate reader
Detect dual luciferase expression on the FlexStation 3 microplate reader
Reporter gene assays are important tools for studying gene expression associated with the activation of cellular pathways. Cells are transfected with a plasmid containing the reporter…
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Application Note
ß-Lactamase-Inhibitor Protein Binding Affinities: Detection of Hydrolysis of ß-Lactam Substrate by TEM-1 Enzyme and Inhibitor Binding Using The FlexStation® 3 Microplate Reader
ß-Lactamase-Inhibitor Protein Binding Affinities: Detection of Hydrolysis of ß-Lactam Substrate by TEM-1 Enzyme and Inhibitor Binding Using The FlexStation® 3 Microplate Reader
Bacterial resistance to ß-lactam drugs through the production of class A ß-lactamase enzymes is an increasing concern in the medical community.1 ß-lactamase inhibitory protein (BLIP)…
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Application Note
Optimization of a muscarinic M3-receptor assay using frozen CHO cells on the FlexStation 3 reader
Optimization of a muscarinic M3-receptor assay using frozen CHO cells on the FlexStation 3 reader
Cell-based assays can often be challenging and time consuming. To facilitate and streamline this complicated process, frozen cells, which can be assayed without prior cultivation, have…
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Application Note
Comparison of Photina luminescent calcium mobilization assays on the FlexStation 3 reader and FLIPR Tetra System
Comparison of Photina luminescent calcium mobilization assays on the FlexStation 3 reader and FLIPR Tetra System
G protein-coupled receptors (GPCRs) represent one of the most important therapeutic targets in drug discovery research. GPCRs are membrane-localized proteins that play an important…
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Application Note
Gq-coupled receptor assays using the FlexStation System: M1 muscarinic receptor
Gq-coupled receptor assays using the FlexStation System: M1 muscarinic receptor
Gq-coupled receptors are transmembrane proteins that are responsible for transmitting extracellular signals to the inside of the cell, which ultimately leads to changes in gene…
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Application Note
Monitoring receptor mediated changes in [Ca2+] i using single- and dual-wavelength indicators on the FlexStation 3 reader
Monitoring receptor mediated changes in [Ca2+] i using single- and dual-wavelength indicators on the FlexStation 3 reader
Calcium (Ca2+) is the most common signal transduction element in cells ranging from bacteria to specialized neurons. Measurement of changes in the concentration of intracellular…
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Application Note
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease,…
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Application Note
Calcium signaling with FLIPR Calcium 6 and 6-QF Assay Kits on the FlexStation 3 reader
Calcium signaling with FLIPR Calcium 6 and 6-QF Assay Kits on the FlexStation 3 reader
FLIPR® Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive fluorescent screens…
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Application Note
IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers
IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers
Cyclic nucleotide phosphodiesterases (PDEs) are a group of enzymes that degrade the phosphodiester bond of cAMP and cGMP, second messengers that are involved in a variety of biological…
eBook
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
This new eBook discusses early drug safety testing using HTS iPSC-derived cells with imaging. Download it now!
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eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
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eBook
Fast Track Cell-Based Assays Like a Pro
Fast Track Cell-Based Assays Like a Pro
Our FlexStation® 3 Multi-Mode Microplate Reader is a multi-detection platform that increases liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays.
Brochure
SoftMax Pro 7 Data Acquisition and Analysis Software
SoftMax Pro 7 Data Acquisition and Analysis Software
Learn how SoftMax Pro 7 Data Acquisition Software provides a new data analysis features, while keeping its intuitive layout.
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Brochure
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. Our…
Scientific Poster
Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes unnecessary proteins by breaking them down into short peptides. It consists of a…
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Scientific Poster
Dual Luciferase® Reporter Assay Detection on Next-Generation FlexStation® 3 Microplate Reader with Integrated Fluidics
Dual Luciferase® Reporter Assay Detection on Next-Generation FlexStation® 3 Microplate Reader with Integrated Fluidics
Learn how the FlexStation 3 can be used to accurately and easiy run Dual Luciferase® Reporter Assays using the integrated fluidics and sensitive optics of the Flexstation 3 system.
Videos and Demos
Getting Started with Fluorescence Polarization
FlexStation 3 Multi-Mode Microplate Reader
FlexStation® 3 Series Multi-Mode Microplate Readers
| Product | Part Number |
|---|---|
| FlexStation 3 Microplate Reader with SoftMax Pro Software Includes: FlexStation 3 Base System, 1 year warranty covering parts & labor. **Does not include 8 or 16 channel pipettor head, must be purchased separately and MUST be ordered at the time of ordering the instrument. | Flex3 |
| SpectraDrop™ Micro-Volume Starter Kit | 0200-6262 |
| SpectraDrop™ Micro-Volume HTS Kit | 0200-6267 |
| FlexStation 3 Benchtop Multi-Mode Microplate Reader IQ/OQ Validation Protocols | 0112-0160 |
Related Products & Services of FlexStation 3 Multi-Mode Microplate Reader
Featured Applications
Enzyme Activity
Enzyme activity can be monitored in real time using a chromogenic substrate which, upon its…
Fast Kinetic Calcium Mobilization Assays
Gq protein-coupled receptor (GPCRs) activation can be monitored by measuring the increase in…
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level…
Customer Breakthrough
SUCCESS STORY
The University of Cambridge uses the FlexStation 3 reader to study the structure and function of calcium signalling pathways
Interactions between Ca2+ and cAMP signalling in human vascular smooth muscles.
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