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Explore automation-ready systems for your ELISA workflow


ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. The assay is easy to set up and the range of potential analytes is vast, but the assay procedure is time consuming and labor intensive.

Laboratory automation workflows can help with providing walkaway time, increasing throughput, effectiveness and efficiency of the assay procedure, and reproducibility. 

  • Biomek i7 automated liquid handler executes dispensing reagent or samples via multichannel head.
  • Using an automated heat sealer, a sealing film will be placed on the assay plate to prevent evaporation during incubation.
  • The incubation is done at room temperature in a LiCONiC LPX44, that can store to 44 microplates and while shaking them during the incubation period.
  • After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing.
  • Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head.
  • Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader.
  • SoftMax® Pro Software will automatically generate a standard curve and calculate target antigen concentration in unknown samples.

Fully-integrated, automated ELISA workflow

The Molecular Devices Automation & Customization Team has successfully tailored microplate readers for customers to customize software capabilities and to integrate with other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell.



ELISA Workflow

Integrated workcell lab components:

  • Molecular Devices labware hotels
  • AquaMax Microplate Washer
  • SpectraMax Microplate Reader
  • SoftMax Pro GxP Software
  • Beckman Coulter Biomek i7 automated liquid handler
  • LiCONiC Wave STX44 automated CO2 incubator
  • Precise Automation PreciseFlex 400 robot
  • Automation scheduling software
  • Plate sealer and remover
  • Automated shaker



Resources for Lab automation for high-throughput ELISA workflow

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