High-throughput screening for GPCRs and Ion Channels
The FLIPR Tetra® High-Throughput Cellular Screening System provides an automated solution for identifying early leads in the drug discovery process and for evaluating drug efficacy and toxicity. With simultaneous pipette and read function, the system supports fast kinetic cellular assays. It can be quickly configured based on library size, detection mode, screening format, assay and target. As a fully integrated solution, the transition from assay development to lead optimization is seamless.
Enable more assays
The system supports an incredible range of assays including calcium, cardiotoxicity, potassium, and membrane potential. Design custom protocols or choose from our variety of turnkey kits.
Screen with multiple detection modes
Seven fluorescence LED sets with many filter options and optional aequorin luminescence detection available for single wavelength and ratiometric assays. Easily switch between fluorescence and luminescence read modes.
Configure protocols easily
ScreenWorks® Software simplifies the assay development process by presenting options consistent with corresponding fluidics and optics in a drag-and-drop user interface.
FLIPR Tetra High-Throughput
User-changeable pipette modules
Pipette heads can be exchanged in minutes without the use of tools. Easily change the pipettor head from 96, 384, or 1536 channels to accommodate assay throughput needs.
Configurable excitation and emission optics allow for expanded dye capabilities. User-configurable optics support rapid optical changes, beneficial in multi-user, multi-assay environments.
Advanced data handling
View signal traces, EC50 curves, and Z factors on-the-fly without having to export data to another software program. The system provides rapid analysis of compound efficacy or toxicity.
Multi-peak data analysis
Optional ScreenWorks PeakPro Software simplifies the characterization of calcium oscillations in primary cells and stem cells. It offers analysis of multi-peak data and 15 extra analysis parameters.
Internal plate handler
The FLIPR TetraCycler™ Plate Handler supports microplate and tip changes, and facilitates integration into larger laboratory robotics systems supporting ultra high-throughput drug discovery.
Custom automation options*
The Automated Workflow Engineering Solutions Team can offer a variety of customized services from automated compound plate delivery to fully integrated workstations with automated incubation.
*Price, time to deliver, and specifications will vary based on technical requirements agreed upon. Solution requirements may cause adjustment to standard performance.
Applications of FLIPR Tetra High-Throughput Cellular Screening System
Early prediction of drug-induced functional cardiotoxicity requires robust in vitro systems suitable for high-throughput screening. Readily-available iPSC-derived human cardiomyocytes may be used in conjunction with calcium-sensitive dyes, and beat rates are monitored as changes in intracellular calcium. These calcium peaks can be analyzed with our ScreenWorks PeakPro Software which provides the investigator a suite of powerful tools for quantification of cardiomyocyte activity.
The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity. Calcium flux can be used as a measurement for a host of cellular processes including neurotransmitter release, GPCR activity, voltage or ligand gated ion channels, and cardiomyocyte beat patterns, among many others. The FLIPR Tetra System is the drug discovery tool for evaluation of calcium flux in high and ultra-high throughput due to its ease of use, sensitivity and user configurability.
The FLIPR Tetra System is an excellent tool for monitoring calcium flux related to neuronal activity. Newly developed commercially available iPSC neurons have made high-throughput neuronal screening a reality. These cells and assays are essential for monitoring a variety of factors including neural cell culture conditions, drug compound effects, and the effect of environmental neurotoxins. Neuronal activity and neurotransmitter release can be evaluated with fluorescence-based calcium indicator dyes combining fast, simple, and powerful assays with scalable throughput.
There are dozens of identified disorders caused by impaired ion channel function. Ion channels are important drug discovery targets because they are highly druggable and specific. The FLIPR® Membrane Potential Assay Kits provide a fast, simple and reliable fluorescence-based assay for detecting changes in voltage across the cell membrane mediated by ion channel to evaluate target or off-target effects based on the application.
Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery. These dyes make GPCR screening assays highly sensitive, easy to perform, and low cost compared to other assays. Instruments capable of measuring fluorescent-based calcium indicators in high-throughput have become significantly more affordable making them obtainable even for academic groups.
Functional evaluation of potassium ion channels in a cell is of critical importance in the drug discovery process, particularly when it involves cardiac safety. The FLIPR® Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+ indicator dye produces a bright fluorescent signal proportional to the number of potassium channels in the open state providing a functional indication of the potassium channel activities
Specifications & Options of FLIPR Tetra High-Throughput Cellular Screening System
Resources of FLIPR Tetra High-Throughput Cellular Screening System
Shorten growth timelines when screening compound effects on calcium transients in beating cardiomyocytes by using the iCell® Cardiomyocytes2 Kit (Cellular Dynamics International).…
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FLIPR Tetra System
Drug-induced inhibition of the human ether-à-go-go-related gene (hERG) ion channel has been related to the susceptibility of patients to potentially fatal ventricular tachyarrhythmia,…
Comparison of Photina luminescent calcium mobilization assays on the FlexStation 3 reader and FLIPR Tetra System
G protein-coupled receptors (GPCRs) represent one of the most important therapeutic targets in drug discovery research. GPCRs are membrane-localized proteins that play an important…
ScreenWorks Peak Pro Software: Visualize and analyze the temporal response curves associated with changes in intracellular calcium ion concentration. The new analysis features simplify…
FLIPR® Membrane Potential (FMP) Assay Kits provide a rapid and reliable fluorescence-based method to detect changes in membrane potential brought about by compounds that modulate or…
Measuring GPCR Activity Using the Tango GPCR Assay on the FLIPR Tetra Fluorimetric Imaging Plate Reader System
The FLIPR Tetra System has long been used for uHTS and HTS of fast-kinetic GPCR and ion channel assays. The limitations of current microplate readers with respect to format and…
In this application protocol we demonstrate the use of the modified luminescent firefly luciferase-based Promega GloSensor™ cAMP Assay on the FLIPR Tetra® High Throughput Cellular…
Combining a novel fluorophore and proven masking technology, the FLIPR Calcium 5 Assay Kit delivers reliable pharmacology, a larger signal window, and improved assay performance. With…
Eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process with the FLIPR Tetra System, together with ScreenWorks Peak Pro…
The FLIPR Tetra System delivers a screening platform to address real-time kinetic cell-based assays in 96-, 384- and 1536-well format. Regardless of the FLIPR Tetra System microplate…
In this study, we demonstrate the novel utility of optogenetic tools to control membrane potential in a reversible and precise fashion for screening state-dependent calcium channel…
Development of a cell-based potassium-chloride transporter assay using the FLIPR Potassium Assay Kit
The FLIPR Potassium Assay Kit can be used to measure the functional activity of the hKCC2 cation-chloride cotransporter using a homogeneous, no-wash protocol. The assay kit displays a…
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
The assessment of cardiotoxicity is important in the early stages of drug discovery to enable elimination of potentially toxic compounds from further development. This is critical to…
This document provides the information about FLIPR Tetra Instrument LED and Filter Combinations. Explore this document for more detailed information.
Explore this data sheet to learn about Pin tool liquid handling for FLIPR Tetra system which allows direct transfer of compounds that have been suspended in 100% DMSO or ethanol.
Explore PDF to learn about a broad range of optics for FLIPR Tetra System that enable application you require to support your research & screening.
Evaluation of Cryopreserved Bacmam Transduced Aequorin Cells in 384-and 1536-Well Suspension Cell Assays Using the Aequorin Option for the FLIPRTETRA® System
Explore PDF to learn about the FLIPR TETRA suspension cell system that provides an effective method to perform Aequorin assays over an extended period of time enabling robust HTS.
Read this PDF to know how fluorescence-based assay kit is useful for the measurement of neurotransmitter transporter uptake activity in cell lines stably expressing the human DAT, NET,…
Read this PDF to explore LED light sources with wavelengths at 340 nm and 380 nm which enable ratiometric Fura‐2 measurements on the FLIPR Tetra system.
Calcium Release-Activated Channel (CRAC) Assays on the FLIPR® Tetra System: Evaluation of a Novel Fluorescent Calcium Reagent
Get detailed information about Calcium release-activated calcium channels by exploring this PDF. Learn how CRAC channels are playing an important role in intracellular Ca2+ homeostasis.
Cell Based Assays on the FLIPR® Tetra System: Comparison of a Novel FLIPR® Calcium Assay to other Fluorescence based Calcium Flux Assays
Cell based assays on FLIPR Tetra System to compare a novel FLIPRCcalcium 6 and 6-QF assay to other fluorescence-based calcium flux assays.
This PDF explores live cell assays for measuring the impact of pharmacological compounds on rate & magnitude of beating cardiomyocytes using high content imaging & electrophysio…
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Learn about cell assays for measuring the impact of pharmacological compounds on rate & magnitude of beating cardiomyocytes using FLIPR Tetra & ImageXpress Micro Systems.
Ion channels are a class of membrane proteins that mediate the movement of charged ions across the cell membrane. Potassium channels constitute the largest and most diverse group of ion…
Videos and Demos
Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay
Latest Advances in Calcium Flux Assays
Innovative Assays for the FLIPR Tetra System
Developing an in Vitro Tl+ Flux Assay for Potassium Channel Agonist Identification
Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery
FLIPR Tetra High-Throughput Cellular Screening System
|FLIPR Tetra High-Throughput Cellular Screening System||FLIPR|
|FLIPR Tetra Camera Conversion Kit, EMCCD to ICCD||0310-5285|
|FLIPR Tetra Cell Suspension, Field Installation Kit||0310-5339|
|FLIPR Tetra TETRACycler||Tetra+Cycler|
|FLIPR Tetra Pipettor Head Kit, 96||0200-6071|
|FLIPR Tetra Pipettor Head Kit, 384||0200-6072|
|FLIPR Tetra Pipettor Head Kit, 1536||0200-6073|
|1FLIPR Tetra Pin Tool Head Kit, 384||384 Pin Tool Hd Kit|
|1FLIPR Tetra Pin Tool Head Kit, 1536||1536 Pin Tool Hd Kit|
|FLIPR Tetra Pipette tips, black, non-sterile, 96-well 50 racks/case||9000-0762|
|FLIPR Tetra Pipette tips, clear, non-sterile, 96-well 50 racks/case||9000-0761|
|FLIPR Tetra Pipette tips, black, non-sterile, 384-well 50 racks/case||9000-0764|
|FLIPR Tetra Pipette tips, clear, non-sterile, 384-well 50 racks/case||9000-0763|
|FLIPR Tetra 1536 tip gasket, non-sterile, 40/case||9000-0746|
|FLIPR Tetra 1536 tip block||0200-6112|
|1FLIPR Tetra Pin Tool head, 384||384 Pin Tool|
|1FLIPR Tetra Pin Tool head, 1536||1536 Pin Tool|
|LED Modules and Emission filter accessories are available for both versions of the FLIPR Tetra instrument for Calcium sensitive dyes, Membrane Potential dyes, Voltage Sensor dyes and others applications. Please follow this link to a .pdf of the wavelengths and ordering information|
|Optional Cell Suspension System and Consumables|
|FLIPR Tetra Suspension Reservoir||0200-6222|
|FLIPR Tetra 250 mL Spinner Flask Assembly||0200-6223|
|FLIPR Tetra 500 mL Spinner Flask Assembly||0200-6224|
|FLIPR Tetra 1 L Spinner Flask Assembly||0200-6225|
|FLIPR Tetra 2 L Spinner Flask Assembly||0200-6226|
|FLIPR Tetra 3 L Spinner Flask Assembly||0200-6227|
1. Please contact your local Molecular Devices Sales Representative for details regarding this configure to-order item.
FLIPR Tetracycler and Robotic Integration
Optional Cell Suspension System and Consumables
An optional cell management system for the FLIPR Tetra System simplifies luminescent calcium mobilization assays and increases throughput by eliminating the need to plate cells and the need for separate compound plates during aequorin assays. Cells are held in spinner flask which maintains a uniform cell suspension and promotes assay robustness. During the assay, cells are pumped to the reservoir for pipetting and returned to the spinner flask. Adjustable stir speed, pump speeds and pipetting parameters simplify the optimization of cell handling for different cell lines. A variety of flask sizes are available to scale the volume for assay development through high throughput screening. Requires Part number 03010-5339 field installation of Cell Suspension system.
Related Products & Services of FLIPR Tetra High-Throughput Cellular Screening System
The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity.…Read More
Early prediction of drug-induced functional cardiotoxicity requires robust in vitro systems…Read More
Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in…Read More
There are dozens of identified disorders caused by impaired ion channel function. Ion channels are…Read More
The FLIPR Tetra System is an excellent tool for monitoring calcium flux related to neuronal…Read More
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