Overview of FLIPR Membrane Potential Assay Kits

Each homogeneous assay kit utilizes a proprietary indicator dye and quencher combination to maximize cell line/channel/compound applicability, while eliminating causes of data variability. This unique formula responds 10 times faster and has greater temperature stability than traditional dyes, providing high quality screening data that shows good correlation with manual patch clamp assays.

Because ion channel activity is highly sensitive and potentially impacted by subtle chemical changes, two FLIPR® Membrane Potential Assay Kits (Red and Blue) are available to select the optimal conditions for your delicate ion channel targets. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels.

Unlike traditional dyes such as DiBAC, FLIPR Membrane Potential Assay Kit detects bidirectional gradient changes so you can monitor both variable and control conditions within a single experiment. We recommend evaluating both assay kits to discern which formulation is right for your target.

Data of FLIPR Membrane Potential Assay Kits

  • Patch Clamp vs FLIPR Membrane Potential Assays

    Comparison of Patch Clamp and FLIPR Membrane Potential Assay Kit Data

    The manual patch clamp method is able to detect fast responses allowing detection of very rapid changes in membrane potential. Comparing data generated using the FLIPR Membrane Potential Assay Kits with results from the manual patch clamp method shows good correlation (Figures 1 and 2). Both the opening and closing of the ion channel can be observed. This differs from DiBAC, which can only show unidirectional changes in membrane potential (Figure 3).

    Comparison between patch clamp (mV) and FLIPR Membrane Potential (fluorescence) Assays on CHO cells expressing a voltage-gated K+ channel. Data courtesy of Michael Xie, Millenium Pharmaceuticals.

  • Fluorescence vs Membrane Potential Change

    Correlation of changes in membrane potential to fluorescence assays on the FLIPR Instrument: CHO cells transfected with K+ channel exposed to various concentrations of potassium. Data courtesy of Michael Xie, Millenium Pharmaceuticals.

    Fluorescence vs Membrane Potential Change

  • Ligand-gated Calcium Channel Comparison

    Ligand-gated Calcium Channel Comparison

    Comparison between FLIPR Membrane Potential Assay Kit, DiBAC, and Fluo-3 assays on ligand-gated Ca2+ channels. Data courtesy of Michael Xie, Millenium Pharmaceuticals.

  • Two Membrane Potential Kit Quench Formulations Options

    Because ion channel activity is sensitive to interference, and chemical interference with a particular ion channel is highly unpredictable, the FLIPR Membrane Potential Assay Kits have two formulations. Both formulations combine the advantages of our proprietary membrane potential indicator dye with our patented quench technology. One formulation uses a blue quencher and the other formulation uses a red quencher. We recommend that both versions be evaluated for each individual target/cell line to determine which formulation will provide optimal performance.

    Modulation of a Nav1.5 channel in CHL-hH1 cells by tetrodotoxin. In this assay, 30 mM veratridine is used to hold the sodium channel in its open state. Modulation occurs as tetrodotoxin concentration increases. A rapid influx of Na+ into the cell occurs, subsequently depolarizing the membrane, and leading to an increase in fluorescence. In this assay, the Membrane Potential Red Kit showed the larger signal window and larger Z factor.

    Modulation of a Nav1.5 channel

Technology of FLIPR Membrane Potential Assay Kits

  • Fluorescence Intensity Changes with Increase or Decrease in Cellular Membrane Potential

    Fluorescence intensity changes with increase or decrease in cellular membrane potential

    FLIPR Membrane Potential Assay Kits detect ion channel modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. The fluorescent signal increases in intensity during membrane depolarization as dye follows the positively charged ions inside the cell. During membrane hyperpolarization, fluorescent signal decreases in intensity as dye follows the positively charged ions out of the cell. The kits are uniquely suited for use with the simultaneous pipet and read capability of the FLIPR® Tetra System and FlexStation® 3 Microplate Readers to capture fast kinetics associated with ion channel activation.

    The kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology (U.S. patent number 6,420,183, EPO patent number 0 906 572) is offered to drug discovery and life science researchers exclusively by Molecular Devices through the purchase of FLIPR Assay Kits.

Ordering Options of FLIPR Membrane Potential Assay Kits

FLIPR Membrane Potential Assay Kit

Explorer Kit RED

  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8126

$629.00 USD

1

Bulk Kit RED

  • (10) vials of component A*

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8123

$3,213.00 USD

1

Explorer Kit BLUE

  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8042

$629.00 USD

1

Bulk Kit BLUE

  • (10) vials of component A*

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8034

$3,213.00 USD

1

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.