The Challenge
The goal of these studies is to identify mechanisms that are amenable to therapeutics and therefore applicable for preventing memory loss. Dr. Krishnan investigates differential phospholipase D (PLD) expression and the effects of isoforms using a multi-disciplinary approach consisting of electrophysiology, signaling, and behavioral studies.
The Solution
His electrophysiology studies include the use of two patch-clamp rigs, each with a MultiClamp 700B Microelectrode Amplifier and Digidata digitizer. One rig is used for brain slice patch and field recordings in rodent brain slices, while the other is designated for cellular patch recordings. Both patch-clamp rigs take advantage of dual recordings to evaluate the signaling mechanisms underlying memory and recall. Such studies will be instrumental in mapping the brain circuitry affected while simultaneously recording changes at the level of individual neurons.
- Alzheimer’s disease (AD), the most common and severe age-associated neurodegenerative dementia
- Currently there is no cure and the need to identify innovative targets for prevention and treatment are urgently needed
Products Used
Equipped with up to four HumSilencer™ channels, the Axon™ Digidata® 1550B Low Noise Data Acquisition System enables you to record multiple cells at once for cellular network studies without 50/60 Hz line-frequency noise.
The MultiClamp™ 700B Microelectrode Amplifier is a versatile, computer-controlled microelectrode amplifier designed for patch voltage-clamp (resistive-feedback, four ranges) or high-speed current clamp (voltage-follower, three ranges) recording within the same headstage. Many automated functions and effective signal conditioning. Ideal for many applications, including high-speed current clamp (sharp-electrode or field potentials), patch clamp (whole-cell, macro-patch, or excised patch), voltammetry/amperometry, ion-selective measurements and bilayer recordings.
The Results
Dr. Krishnan uses Two MultiClamp 700B Microelectrode Amplifiers, Axon Digidata 1550A System with HumSilencer, Axon Digidata 1332A System, Axon pCLAMP 10 Electrophysiology Data Acquisition and Analysis Software to:
- Uncover peripheral circadian rhythms of smell in Drosophila melanogaster, the first proof of a functional change in the peripheral oscillator governing circadian rhythms.
- Analyze synaptic function in the amygdala, which controls long term memory expression of aversive and appetitive memories.
- Evaluate how disruption of synaptic function in the hippocampus forms the basis of early events in neurodegenerative states resulting in pathological memory loss.
References
View our webinar with Dr. Krishnan: Using Electrophysiological Studies to Accelerate Mechanistic Study in Reception and Transmission
Abstract from his research at the Neuroscience 2016 meeting