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Application Note

Validate CRISPR-Edited Cells using Imaging and Western Blot Detection on a Microplate Reader

  • Measure transfection efficiency by accurately identifying GFP-positive cells vs non-transfected cells
  • Validate CRISPR-mediated gene knockdown using western blot analysis on protein bands
  • Capture high-quality western blot images
  • GFP 阳性细胞 Vs 未转染细胞精 确判断转染效率
  • 使用免疫印迹分析蛋白条带方 式验证 CRISPR 介导基因敲除技 术
  • 获得高质量 Western Blot 图像