Application Note

Rapid selection of adherent cell lines using ClonePix Technology

  • Save time over traditional methods such as limiting dilution with a rapid one-step process
  • Select clones/cell lines with optimum expression levels that cannot be forced into suspension
  • Detect intrinsic and cell surface proteins by direct fluorescence

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Introduction

During the last decade, production of monoclonal antibodies and recombinant proteins by mammalian cells has become a cutting edge technology. However, the generation of transient as well as stable cell lines has been a major challenge for many cell types since overall transfection efficiencies and/or integration frequencies reveal considerable variation. Selection of high-productivity clones in a transfected pool of cells poses a major bottleneck in cell-line development and manufacturing of recombinant proteins and monoclonal antibodies (mAbs). As transfected pool of cells is teeming with low producers, and high producers are rare, spotting a desirable high-producing clone is tantamount to finding a needle in a haystack.

ClonePix™ System technology and associated reagents represent a well-established one-step solution for the identification and isolation of rare clones from large heterogeneous cell populations without the need for labor- and time-intensive limiting dilution (manual methods) or other cumbersome cell screening techniques. The ClonePix System captures both white light and fluorescent images in situ to quantify the fluorescent protein expression levels with capabilities of picking both suspension and adherent cells. The latter, such as HEK 293 and CHO-K1 cell lines, require an efficient and gentle picking process to retain cell viability for further clonal expansion. In previous years, we have successfully demonstrated the capability of the ClonePix System to perform high-throughput cell selection and product secretion–based clone isolation for suspension cells that are plated at low density in a semisolid medium supplemented with a fluorescently tagged antibody that is specific to the secreted product. High producers are thus identified at the first stage of screening, which subsequently shortens the overall development timeline.

Suspension cells are predominantly used in cell line development, however the suspension cell lines have traditionally proven to be difficult to transfect. One main advantage of using adherent cells is the ease of transfection, especially when testing various reporter constructs. Furthermore, the screening process can be a lot easier for adherent cell selection with the aid of ClonePix System, based on the intracellular or cell surface expression of reporter protein. Additionally, the ClonePix System can be useful to identify and isolate clones expressing recombinant proteins apart from hybridomas/cell lines secreting monoclonal antibodies. The desirable adherent clones identified by intracellular or cell surface fluorescence intensity would thereby indicate clones with stable protein expression and/or correct protein conformation.

Two adherent cell lines, one expressing green fluorescent protein and the other red fluorescent protein were used to evaluate the efficacy of the ClonePix 2 System with a range of adherent cell picking reagents of increasing stringency for use in cell culture. Here we present a workflow for adherent cell picking using the ClonePix System in which distinct clones can be imaged with the specific fluorescence channels, analyzed and picked proficiently. Data illustrated here display sustaining cell viability post-picking and clonal expansion with subsequent culturing into 96-well plates.

Materials

Cell lines

  • HEK-293 adherent cell lines stably expressing fluorescent proteins obtained from GenTarget Inc.
    • HEK293-green fluorescent protein/GFP (GenTarget Inc., Cat. #SC001)
    • HEK293- red fluorescent protein/RFP (GenTarget Inc., Cat. #SC007)

Adherent Cell Picking Reagents

  • Adherent Cell Picking Reagent Test Kit types A, B, C, and D (Molecular Devices, Cat. #K8005)
    • A: very low stringency
    • B: low stringency
    • C: medium stringency
    • D: high stringency

ClonePix 2 System

  • Automated mammalian cell colony picking system
  • Image colonies in white light and fluorescence
  • Trans-illumination supports imaging low contrast colonies such as adherent monolayers
  • Software-controlled switching between up to five excitation/emission filter pairs
  • Ranks colonies using user-definable criteria
  • Target colonies identified and picked into 96-well destination plates
  • Support multiple applications for both suspended and adherent cell lines including:

CloneSelect™ Imager

  • Label-free white light imaging of cells
  • Objective, quantitative assessment of cell growth
  • Simple, user-friendly software interface
  • Growth rates accurately determined in every well of a 96-well plate
  • Supports multiple applications including:
    • Monitoring cell growth
    • Verification of monoclonality

Methods

Plating of HEK-GFP and HEK-RFP co-cultures

The HEK-GFP and HEK-RFP cells were mixed at equal ratios in a range of low- to high-cell densities (25 cells/well – 300 cells/ well) and plated on a series of 6-well tissue culture treated plates to encourage formation of adherent colonies. The cells were co-cultured for seven days in liquid tissue culture media at 37°C, 5% CO2 , monitored periodically for colony formation. Low plating density resulted in forming discrete clonal colonies.

Adherent cell screening and picking with ClonePix 2 System

Upon successful formation of colonies (typically 7-10 days post-plating), the following adherent picking protocol was implemented using the adherent cell picking reagent test kit (Molecular Devices Cat. #K8005). The four Adherent Cell Picking Reagents from the kit (A, B, C, and D) comprise a range of increasing stringency from very low to high. The appropriate stringency conducive for gentle plus efficient cell dissociation and picking was evaluated empirically with each respective picking reagent solutions A, B, C, and D.

Picking protocol

  1. Pour off growth medium from cell colonies.
  2. Dispense an equal volume of Adherent Cell Picking Reagent into the dish/well.
  3. Pour off Adherent Cell Picking Reagent.
  4. Leave at room temperature for a minimum of 10 minutes before picking colonies.
  5. Gentle wash with PBS. Suspend in liquid tissue culture media.
  6. Using the ClonePix 2 System, imaging and selection of colonies performed with the aid of user defined criteria.
  7. Selected colonies were picked using F2 pins (700 mm diameter) with ‘Adherent’ Pin Option in Picking Settings to ensure the pin descends fully to the base of the plate. (F2 pins have a wider diameter as compared with F1 pins, and therefore allow more cells to be picked up from the bottom of the plate).
  8. Following user-specified picking, each colony was transferred to individual wells in a 96-well destination plate for expansion of clonal cells. The growth/confluence was monitored periodically for seven days using CloneSelect Imager.

Note: K8005 is a test kit of 4 different picking reagents—the optimum stringency for efficient picking and survival post-picking would be contingent upon a particular cell line).

Fluorescence-based image analysis and custom clone selection

For screening and selection of colonies, ClonePix 2 System enabled the imaging in white light and multiplex fluorescence channel. Using userdefined selection criteria, the colonies were ranked according to interior mean fluorescence intensity

Figure 1. GFP and RFP clones

Figure 2. Colony ranking and grouping

Figure 3. Tracking colonies                                                                    Figure 4. Picked colonies

Figure 5. Monitoring growth/confluence post-picking

The growth/confluence was monitored periodically from 0–seven days post-picking using CloneSelect Imager. Cell recovery and expansion after picking was recorded by growth confluence and growth curve (at right) using CloneSelect Imager. Illustration of growth curves demonstrate up to 98% confluence on day seven for wells picked with adherent picking solutions type C and D.

Data display

Imaging software generates a 2-D map of clones and their secretion levels from a series of images generated in situ. Screening and selection of colonies is based upon several criteria:

  • Size, roundness and proximity to neighbors
  • Ranking according to fluorescence levels
  • Closely placed colonies ignored via usercontrolled “proximity” software setting

Colonies displaying high levels of interior mean fluorescence were selected for picking based on three groups—green only, red only, plus both green and red fluorescence expression. The picked colonies were transferred to a 96-well plate for expansion. Isolated clones were monitored for viability and growth using CloneSelect Imager.

Tracking cell growth using CloneSelect Imager

To investigate the rate of viability of the picked cells, CloneSelect Imager was used to monitor cell growth in the 96-well destination plates for seven days post-picking. Good growth was seen with wells achieving up to 98% confluence within seven days. The picking reagents type C and D were optimal as compared to type A and B, for the cell lines used in this study as demonstrated by 100% survival and 98% cell expansion/confluence in the corresponding picked wells. The picking treatment with reagents type A and B were unsuccessful in disrupting the attachment of the adherent clones from the base of the plate. For efficacious picking of different types of adherent cell lines, the optimal picking reagent stringencies can vary depending on intrinsic properties of the cell lines.

Validation of fluorescence based clone selection

Accuracy of picking was validated by imaging wells using the ImageXpress® Micro Widefield High Content Screening System. Of the HEK-GFP and HEK-RFP classified picked wells, 98% of clones demonstrated either GFP or RFP only respectively. This demonstrates the ClonePix 2 System’s ability to accurately differentiate and select between the green and red fluorescence with a high level of confidence.

Figure 6. Wells picked as HEK-GFP and HEK-RFP only

Summary

Mammalian cell culture technology has greatly expanded its application from basic research to the biopharmaceutical industry for the manufacturing of biotherapeutic agents, such as vaccines, monoclonal antibodies, and bioactive recombinant proteins. ClonePix Technology provides a rapid solution for picking adherent cells while maintaining viability that is critical for cell line expansion downstream. The picking process on the ClonePix System is independent of enzymatic digestion of cell-surface proteins (such as trypsin-treatment), and thereby enables quick and gentle picking of colonies while maintaining clonality.

A range of four Adherent Cell Picking Reagents of increasing stringency (A-D) described here, are effective on a wide range of anchorage dependent cell lines, enabling detachment and picking of clones on the ClonePix System. The optimal picking efficiency in relation to the stringency of picking reagents varies with different cell lines and would be dependent on the particular intrinsic characteristics of the cell lines, and needs to be determined empirically. Furthermore, as these picking reagents are chemicallydefined and free of animal products, it provides an excellent non-animal derived ready-to-use alternative for complying with ever increasing regulatory requirements.

Imaging and clone selection criteria on the ClonePix System provide accurate differentiation between the green and red fluorescent expression as validated in combination with the ImageXpress Micro System. ClonePix Technology, offers rapid, one-step solution for optimal clone selection, thereby aiding in enhancing the future of adherent mammalian cell culture technology in the biopharmaceutical industry.

 

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