| Continuous single electrode voltage-clamp (cSEVC) | discontinuous single electrode voltage-clamp (dSevc) | |
|---|---|---|
| Pipette | The same pipette is used simultaneously for voltage recording and current passing | The same micropipette is used and shares time to inject current and record voltage |
| Cell Size | Better with smaller cells | Better with larger cells as signal is noisier with small cells |
| Optimal Current Range | Currents <5 nA due to noise | Currents must be modest or large in size >5 nA |
| Resistance Compensation | Series resistance compensation performed by amplifier | Series resistance compensated by sampling rate and capacitance compensation |
| Voltage at the Tip of the Electrode | Voltage at the tip of the electrode and the ruptured patch benefits from series resistance compensation by the amplifer to achieve command voltage | Voltage at the tip of the electrode (within the cell) is clamped to the command voltage |
| Voltage at the Top of the Pipette | The voltage at the top of the pipette is controlled by a voltage-clamp circuit. It is the sum of the membrane potential and the current-induced voltage across the pipette | Works better with a larger access resistance (from both pipette and ruptured patch) |
| Available Axon Amplifier(s) | Axopatch 200B and MultiClamp 700B (whole-cell patch mode) | Axoclamp 900A |
| Additional Information | What is continuous single electrode-voltage clamp (cSEVC)? | What is discontinuous single electrode-voltage clamp (dSEVC)? |
For more information, please download our Axon Guide.
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