Comparison between continuous (cSEVC) and discontinuous (dSEVC) single electrode voltage-clamp

  Continuous single electrode voltage-clamp (cSEVC) discontinuous single electrode voltage-clamp (dSevc)
Pipette The same pipette is used simultaneously for voltage recording and current passing The same micropipette is used and shares time to inject current and record voltage
Cell Size Better with smaller cells Better with larger cells as signal is noisier with small cells
Optimal Current Range Currents <5 nA due to noise Currents must be modest or large in size >5 nA
Resistance Compensation Series resistance compensation performed by amplifier Series resistance compensated by sampling rate and capacitance compensation
Voltage at the Tip of the Electrode Voltage at the tip of the electrode and the ruptured patch benefits from series resistance compensation by the amplifer to achieve command voltage Voltage at the tip of the electrode (within the cell) is clamped to the command voltage
Voltage at the Top of the Pipette The voltage at the top of the pipette is controlled by a voltage-clamp circuit. It is the sum of the membrane potential and the current-induced voltage across the pipette Works better with a larger access resistance (from both pipette and ruptured patch)
Available Axon Amplifier(s) Axopatch 200B and MultiClamp 700B (whole-cell patch mode) Axoclamp 900A
Additional Information What is continuous single electrode-voltage clamp (cSEVC)? What is discontinuous single electrode-voltage clamp (dSEVC)? 

For more information, please download our Axon Guide.

Other links:

Return to Patch-Clamp Basics >>