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Antibody Discovery Using Phage Display

Phage Display Workflow

Phage Display is a technique used to study the interaction of proteins displayed on the surface of a bacteriophage with other molecules such peptides, DNA, and other proteins. Phage display is commonly used to find high affinity interactions between antibodies and antigens, which play a critical role in viral pathogenesis, vaccines, and other treatments.  

 

Antibody discovery workflow using phage display
 

Step 1: Panning

Panning is an iterative process for enriching phage within a population that possess high affinity binding to a target of interest compared to others.
 

Step 2: Colony picking 

Bacteriophage selected from the previous step are then cloned and picked in order to isolate each unique protein binder.
 

Step 3: Ag-antibody interactions 

During panning, phages displaying proteins with higher binding affinity are selected in relation to phages displaying lower affinity proteins. This qualitative selection process requires validation using more quantitative immunoassays to assess antibody-antigen interactions such as ELISA, immunofluorescence, HTRF, complement fixation, agglutination, and/or precipitation.
 

Step 4: Functional screening

Following the characterization of antibody-antigen interactions, candidate molecules are then screened for functional activity (e.g. viral neutralization or vaccine efficacy), often using cell-based assays.
 

 

Systems to accelerate your COVID-19 research

Get up and running rapidly with proven technologies

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AquaMax Microplate Washer

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SpectraMax i3x Multi Mode Microplate Reader

Our SpectraMax® series of microplate readers measure absorbance, fluorescence, and luminescence plus additional detection modes with available upgrades for western blot, cell imaging, and fast kinetics with injectors. GMP/GLP labs can meet FDA guidelines with our SoftMax Pro GxP compliance and validation solution.

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Softmax Pro Gxp Software

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QPix 420 Microbial Colony Pickers

The QPix™ 400 Series Microbial Colony Pickers combine intelligent image analysis with precise automation for fast and efficient screening of large libraries. Capable of picking up to 3000 colonies per hour, it will streamline your workflow.

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ClonePix 2 Mammalian Colony Picker

The ClonePix™ 2 Mammalian Colony Picker is a fully automated system for the selection of high-value clones used in antibody discovery and cell line development.

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fsight

The CloneSelect™ Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge.

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CloneSelect Imager

The CloneSelect™ Imager is a high-throughput automated solution for imaging and analyzing mammalian cells.

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ImageXpress Pico Automated Cell Imaging System

Our ImageXpress systems offer an end-to-end solution for high-content screening and analysis. All our systems support a wide range of applications, increased throughput, and streamlined workflows.

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Resources related to COVID-19 and viral research applications

Learn more about how our technology solutions can help support your research of COVID-19 cellular responses and vaccine development.

Systems to accelerate your COVID-19 cellular response and vaccine development

We have validated and compliant laboratory solutions including microplate readers, microplate washers, biopharma and cellular imaging systems to meet your research needs.