Cell line development for recombinant proteins
In order to generate high yields of recombinant protein products, cell lines such as CHO, Hybridoma or HEK 293 are typical platforms of choice. The process of developing stable cell lines starts with transfecting host cells (CHO or HEK 293) with recombinant plasmids or cell fusion for hybridomas. After transfection or cell fusion, large numbers of clones are screened and selected on the basis of expression of protein of interest (therapeutic biologics). Once candidate clones are identified, each “hit” is confirmed, validated, and characterized using DNA sequencing and a variety of downstream analytical assays. Upon completion, the lead clones are expanded and scaled up where bioprocesses occur.
Stages of a typical cell line development process
Workflow for generating recombinant proteins
- Transfection - Transfect host cells with recombinant plasmids encoding a protein of interest.
- Selection of Pool of Transfected Cells - Select cells that are stable and producing protein of interest.
- Clonal Screening and Selection - Screen and select clones for high expression of protein of interest.
- Cell Line Characterization - Validate and characterize each clone for stability and productivity.
- Expansion and Downstream Evaluation - Expand clones and perform downstream bioprocesses. Cell banking is also performed.
Featured assets to optimize your cell line development workflows
Resources for Cell Line Development Workflow
Application Note
Accelerating Gene Edited Cell Lines with the CloneSelect Imager FL
Accelerating Gene Edited Cell Lines with the CloneSelect Imager FL
Gene editing technology allows the manipulations at the DNA level where it is deleted, added, or modified using various tools.
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How to Develop Monoclonal Cell Lines for Stem Cell Engineering
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
Monoclonal assurance is undeniably critical in cell line development for generating therapeutics, such as monoclonal antibodies or cell-based therapies. To utilize a cell line for…
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Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
The market for biotherapeutics and therapeutic proteins was valued at $93.14 billion in 2018 and is expected to nearly double by the end of 2022.
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The evolution of CHO cells’ role in cell line development
The evolution of CHO cells’ role in cell line development
Since the first approval of recombinant insulin and human growth hormone in the early 1980s, a multitude of recombinant protein therapeutics have been approved by regulatory…
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High assurance monoclonality for cell line development
High assurance monoclonality for cell line development
Double confirmation of clonality. Image based evidence confirming single cell deposition event, and single cell in 96- and 384-well plate post sort
eBook
Optimize your high-value cell lines
Optimize your high-value cell lines
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to the mark…
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Streamline the workflow for measuring IgG in cell line development
Streamline the workflow for measuring IgG in cell line development
The process of cell line development for the production of antibody-based therapeutics can vary widely amongst researchers. The ultimate goal is to find a clone that stably produces large…
Brochure
Molecular Devices Advancing your Cell Line Development Workflow through Automated and Proven Technologies
Molecular Devices Advancing your Cell Line Development Workflow through Automated and Proven Technologies
QPix™ systems earned a well-deserved reputation for performance and reliability at major sequencing centers throughout the Human Genome Project. Get ready to discover why research institutes…
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Brochure
Accelerate Cell Line Development
Accelerate Cell Line Development
Explore PDF to learn how you can accelerate your cell line development and get to your high value hits faster.
Videos & Webinars
The many and varied workflows of cell line development
