Stem Cell Applications

Drug-induced organ toxicity is an important cause of pharmaceutical candidates failing to make it to market. Thus, highly predictive assays for safety and efficacy testing are crucial for improving drug development and reducing drug candidate attrition late in clinical trials.

Technology and methods can differ depending on the type of information needed and the cell type used, but the basic workflow remains similar.

Stem Cell Applications Workflow

A method of growing stem cells is to use cell colony pickers to automate the growth of primary cells. The colony picker gently picks and transfers selected stem cell colonies. After this stage, pluripotent stem cells can be induced to differentiate into specific cell types like neurons, cardiomyocytes, hepatocytes, hematopoietic cells, ocular cells, and other cell types. Induced pluripotent stem cells (iPSC) provide researchers a more accurate model as these stem cells exhibit typical characteristics and metabolism of mature cells – as compared to using animal stem cells and can be available in large quantities for screening, allowing for more consistent testing parameters.

Cellular imagers can be used to confirm specific differentiation of stem cells. Microplate readers, as well as high-content imaging systems provide a quantitative method to determine the effects of positive and negative factors on these stem cell assays.

Recently, an increased need for more physiologically-relevant data has pushed researchers to explore the use of 3D cell models to gain more in-vivo-like insight into cell responses. These cells can be captured and analyzed best on high-content, confocal imaging systems designed to explore the cell in the x, y, and z-planes.

For screening assays, using a high-throughput system can help to automate characterization earlier in the drug discovery process by identifying compounds with high toxicological effects.

Stem Cell Technology

Our portfolio of instruments is designed to provide more efficient, reliable methods for simplifying, expanding, and automating stem cell research.

ClonePix Technology

ImageXpress Cellular Imaging Systems
Generate multi-parametric data and capture publication-grade images without sacrificing throughput, reliability, or flexibility.

Phenotypic Characterization of Compound Effects on iPSC-derived Cardiac and Liver Spheroids Using Fast Kinetic Fluorescence and 3D Image Analysis
Multi-parameter in vitro assessment of compound effects on cardiomyocyte physiology using induced pluripotent stem cells

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FLIPR High Throughput Screening System

FLIPR Tetra System
Run fast, reliable cellular screening assays to improve characterization of compounds earlier in the drug discovery process and identify early leads against GPCRs and ion channel targets.

Improve cardiomyocyte assay workflow and quality with shorter iPSC2 cell culture time and the FLIPR Tetra System
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human

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SpectraMax Plate Readers

SpectraMax Microplate Readers
Adapt your lab as your needs change by using upgradable multimode plate readers.

Cardiomyocyte beating and cytotoxicity assays on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer
Toxicity Assays Using Induced Pluripotent Stem Cell-Derived Cells

ClonePix Technology

ClonePix Technology
Automatically screen more clones than conventional techniques, select cells with optimal expression levels, and pick colonies with accuracy.

Selecting Clones of Mouse Embryonic Stem Cells

Molecular Devices Assay Kits

Assay Kits
For best performance and fast results, optimize your stem cell assays with our assay kits, including cardiotoxicity kits.

For the latest list of available resources please visit our Library.