SpectraMax Paradigm Multi-Mode Microplate Reader

A compact, capable, and user-upgradeable microplate reader that enables you to do more, with less.

Future-proof your lab with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Get the system that delivers the data you need today, and when you need more, transform your reader with a detection-mode addition upgrade that takes less than two minutes. The SpectraMax Paradigm reader can do absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence measurements. Detection cartridge modularity allows you to configure the system to meet your current needs, while providing flexibility to address future applications.


  • Flexible design allows for single or dual excitation and emission, to address existing needs and future expansion
  • User upgradable microplate reader in < 2 minutes
  • High speed detection
  • Excellent sensitivity and performance
  • Applications at your fingertips


  • Array of detection cartridge capabilities
  • Automatic Z-height optimization (both top and bottom read configurations)
  • Fluorescence measurements no longer require you to adjust the gain
  • Patented LED intensity adjustment
  • Up to 1536-well format
  • Temperature control
  • Monochromator-based absorbance
  • Filter-based fluorescence
  • TUNE Cartridge available for unmatched sensitivity in wavelength tunable detection
  • Custom Cartridge development available to meet your specific research needs
  • SpectraDrop™ Plate protocols enabling micro volume applications measurement are included
  • Seamlessly integrate with StakMax® Microplate Handling Stacker through SoftMax® Pro Software interface


Assay Kits


  • We offer optimized assay kits for use with the SpectraMax Paradigm reader.

SpectraMax® Paradigm® features future-ready applications that can be installed and ready to use in less than two minutes. With over 20 user-installable detection cartridges available, including custom cartridges, users can easily expand their microplate reader to meet their current and future research needs.

  • Fluorescence Intensity
    • DNA/RNA/protein quantitation
    • Endpoint qPCR
    • Green fluorescent protein
    • Receptor-ligand binding
  • Fluorescence Polarization
    • SNP genotyping
    • Protein-protein interactions
    • Drug receptor studies
  • Absorbance
    • DNA/RNA and protein purity
    • ELISA
    • Cell viability
    • Enzyme kinetics
    • Low volume applications
  • Time-Resolved Fluorescence
    • Enzyme Activity
    • TR-FRET Eu+3 chelates
    • Protein-protein interactions
    • Western blot detection
  • Glow Luminescence
    • LIA
    • Reporter gene
    • cAMP
    • Toxicity
    • Screens
    • Kinetics

SMP 7 Software

SoftMax® Pro Software is the most published microplate reader control and microplate data analysis software package available to scientists. The inclusive package of ready-to-run protocols, analysis algorithms, and 21 different curve fit options provides the full solution from data acquisition to analysis to publishing. The FDA 21 CFR Part 11 compliance tools available in SoftMax Pro GxP Software for regulated laboratories deliver a complete solution to minimize validation costs, provide end-to-end chain of custody, and eliminate the need to validate additional analysis software.


General Specifications


Light source
High Powered LED or Flash Lamp
Plate formats
6- to 1536-well
Temperature range
4°C above ambient to 45°C
± 0.75°C
Linear, Orbital
Endpoint measurement
All Modes
Kinetic measurement
All Modes
Well area scanning
All Modes
Wavelength scanning
Dimensions W Ã-H Ã-D cm (in.)
39 (15.4) -45 (17.7) -63 (24.8)
Weight, without cartridges
45 kg (99 lbs)
Power source
100-240 VAC, 2 A, 50/60 Hz
Robotic compatible
Linear dynamic range
> 5 decades


Technical Specifications


Fluorometry (FI) (Fluorescein)
96-well (200 μL)
0.100 fmol
0.020 fmol
384-well (75 μL)
0.100 fmol
0.012 fmol
1536-well (8 μL)
0.030 fmol
0.003 fmol
Fluorescence Polarization (FP) (Fluorescein 1 nM, SD)
96-well (200 μL)
3.0 mP
0.5 mP
384-well (75 μL)
3.0 mP
0.5 mP
1536-well (8 μL)
6.0 mP
1.5 mP
Time Resolved Fluorescence (TRF) (Europium)
96-well (200 μL)
10.0 amol
2.5 amol
384-well (75 μL)
5.0 amol
1.0 amol (100 μL)
1536-well (8 μL)
1.5 amol
0.6 amol
Luminescence (Lum) (ATP-Glow)
96-well (200 μL)
0.50 fmol
0.30 fmol
384-well (50 μL)
0.50 fmol
0.30 fmol
1536-well (8 μL)
0.20 fmol
0.15 fmol
 * For properly functioning, operated and maintained equipment.
Wavelength Range
230-1000 nm
Wavelength Selection
Monochromator (1.0 nm increments)
Wavelength Bandwidth
4.0 nm
Wavelength Accuracy
± 1 nm
Wavelength Repeatability
± 0.5 nm
Photometric Range
3.5 OD
Photometric Resolution
0.0001 OD
Photometric Accuracy
± (2% + 0.010 OD), 0-2 OD @ 405 nm
Photometric Precision
± (1% + 0.005 OD), 0-2 OD @ 405 nm

Related Videos

This video features the SpectraMax Paradigm Multi-Mode Microplate Reader with the TUNE Cartridge.






Related Collateral





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SpectraMax® Paradigm Multi-Mode Microplate Reader



Part Number

SpectraMax Paradigm Multi-Mode Microplate Reader with SoftMax Pro Software PARADIGM
SpectraMax Absorbance (ABS) Detection Cartridge
SpectraMax Tunable Wavelength (TUNE) Detection Cartridge
SpectraMax Multi-mode (MULTI) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) GeneBLAzer Detection Cartridge
SpectraMax Fluorescence Intensity Dual Label (FI-DL) (MultiTox-Fluor) Detection Cartridge
SpectraMax Time Resolved Fluorescence (TRF) Detection Cartridge
SpectraMax Fluorescence Polarization (FP) (fluorescein) Detection Cartridge
SpectraMax Fluorescence Polarization (FP) (rhodamine) Detection Cartridge
SpectraMax Cisbio HTRF Detection Cartridge
SpectraMax Glow Luminescence (LUM) Detection Cartridge
SpectraMax Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge
SpectraMax  Glow Luminescence (LUM) (96) Detection Cartridge
SpectraMax Glow Luminescence (LUM) (384) Detection Cartridge
SpectraMax Dual Color Luminescence (LUM) (BRET2TM) Detection Cartridge
SpectraMax AlphaScreen 384 STD Detection Cartridge
SpectraMax AlphaScreen 384 HTS Detection Cartridge
SpectraMax AlphaScreen 1536 HTS Detection Cartridge

Other Accessories

 Product Name
Part Number
SpectraDrop™ Micro-Volume Microplate Starter Kit
SpectraDrop™ Micro-Volume Microplate HTS Kit
Multi-Mode Validation Plate for SpectraMax® Paradigm® & FilterMax™ F3/F5 Systems

Number of Citations*: 3,750

Latest Citations:
For a complete list, please click here.

Rootstock-induced physiological and biochemical mechanisms of drought tolerance in sweet orange

LP Gonçalves, TFO Alves, CPS Martins… - Acta Physiologiae …, 2016 - Springer
... One unit of SOD activity was defined as the amount of enzyme required to cause
50 % inhibition of reduction of NBT measured at 560 nm on a spectrophotometer
(SPECTRAMax Paradigm, Molecular Devices, Sunnyvale, USA). ...

Involvement of Bcl-2 Signal Pathway in the Protective Effects of Apigenin on Anoxia/Reoxygenation-induced Myocardium Injury

C Chen, H He, Y Luo, M Zhou, D Yin… - Journal of cardiovascular …, 2016 -
Abstract: Apigenin is a type of flavonoids, which has been
demonstrated to protect myocardium agains.

Melanin pigmentation gives rise to black spots on the wings of the silkworm Bombyx mori

K Ito, M Yoshikawa, T Fujii, H Tabunoki… - Journal of Insect …, 2016 - Elsevier
... 2011). Because the absorption maximum of melanin is at 405 nm, the amount of
melanin was determined spectrophotometrically using a SpectraMax Paradigm reader
(Molecular Devices, Sunnyvale, CA) (Akihisa et al., 2010). ...

Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes

KA Tomcik, WJ Smiles, DM Camera, HM Hügel… - European journal of …, 2016 - Springer
... Cell viability was assessed by measuring the fluorescence excited at 550 nm and read at 600
nm using a SpectraMax Paradigm plate reader (Molecular Devices, Sunnyvale, CA). All assays
were performed in quadrupli- cate and independently repeated. Page 3. Eur J Nutr 1 3 ...

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

D Manoil, A Filieri, J Schrenzel, S Bouillaguet - Journal of Photochemistry …, 2016 - Elsevier
... dimethylsulfoxide (DMSO – Sigma-Aldrich, Buchs, CH). The absorbance of both solutions
in the range of 350–650 nm was measured using a Spectramax Paradigm
spectrophotometer (Molecular Devices, CA, USA) (Fig. 1A). A dental quartz ...

[HTML] A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Z ZhangB WangQ Hu, M Sommerfeld… - Biotechnology and …, 2016 - Wiley Online Library
... In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of
heterotrophically grown cells to specific light/nutrient ... Cell growth was monitored by measuring
optical density at 750 nm with a UV-vis spectrophotometer (SPECTRAmax 340PC, Sunnyvale, ...

Optimizing surface-engineered ultra-small gold nanoparticles for highly efficient miRNA delivery to enhance osteogenic differentiation of bone mesenchymal stromal …

M Yu, B LeiC GaoJ YanPX Ma - Nano Research, 2016 - Springer
... China). After further incubation for 5 min, the OD was measured at 610 nm by a
Spectramax paradigm (Molecular Devices). The ... USA). Luminescence measurements
were performed on a Spectramax paradigm (Molecular Devices). At ...

Functional characteristics of the natural polymorphisms of HIV-1 gp41 in HIV-1 isolates from enfuvirtide-naïve Korean patients

YH Shin, CH Yoon, HJ Yang, H Lim, BS Choi, SS Kim… - Archives of …, 2016 - Springer
... to infect cells in triplicate. At 48 h postinfection, the infectivity was determined by
measuring luminescence using a SpectraMax Paradigm Multi-Mode Microplate
Reader (Molecular Devices). IC50 determination for enfuvirtide ...

Protein coingestion with alcohol following strenuous exercise attenuates alcohol-induced intramyocellular apoptosis and inhibition of autophagy

WJ Smiles, EB Parr, VG Coffey… - American Journal of …, 2016 - Am Physiological Soc
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* as of December 6, 2016.  Source: Google Scholar.