SpectraMax MiniMax 300 Imaging Cytometer

Observe the viability of your cells with the field-upgradeable cellular imaging option for the SpectraMax i3/i3x Multi-mode Detection Platform

See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity, cell proliferation, and protein expression. Now with StainFree™ Cell counting Technology, additional brightfield analysis features, and fluorescent green and red detection channels.

The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform  that offers cellular imaging capability.

  • Simplified workflow: Add cellular imaging assays in a small footprint
  • No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using StainFree™ Technology
  • More data: Multi-parametric cell-based assays with brightfield and green and red fluorescent imaging
  • Familiar software: Set up, image, and analyze cells in a snap using SoftMax® Pro Microplate Data Acquisition & Analysis Software
  • Higher sensitivity: Easily measure low cell concentration samples
  • Lower variance: Normalize your plate reader assay data to the number of cells in each well

The SpectraMax MiniMax 300 Imaging Cytometer supports a multitude of cellular imaging applications, including:

  • Cell counting
  • StainFree cell counting
  • Cell proliferation
  • Cell viability and cytotoxicity
  • Marker expression
  • Apoptosis
  • Mitochondrial toxicity



Cell Count/Viability


  • CHO cells were plated in 2X dilutions starting from 32000 cells/well in a 96-well plate
  • Cells were then stained with Calcein AM dye and counted

Cell Counts Segmentation

Cell Count Dilution
Top: The cell counting module of the SoftMax Pro Software MiniMax Imaging Edition displays segmentation mask of the image (left), selected cell-by-cell measurements (middle), and a histogram of objects by average intensity (right). Bottom: The software allows user to graph cell counts of serially diluted CHO cells.


Cell Proliferation/Cytotoxicity


  • Hepatocytes were treated with known compounds causing liver toxicity
  • Proliferation/toxicity effects assessed by measuring total cell area

Cell Area Heatmap

Cell Area Hepatotoxicity
Top: Heat map of hepatotixicity experiment demonstrating dose-wise response to four compounds (blue - low area cells; red - high area cells). Bottom: After image analysis, the software plots results and fits the curves to determine IC50s (red - aflatoxin 1; green - aflatoxin 4; blue - staurosporine; purple - mitomycin C). iCell Hepatocytes from Cellular Dynamics International.


Marker Expression


  • HUVEC cells were pre-treated with anti-inflammatory compounds and then cultured in the presence of inflammatory cytokines
  • Stimulated protein expression was measured by total integrated intensity

Cell Proliferation HUVEC

Cell Proliferation Anti-Inflammatory
Top: Expression of VCAM protein on cells activated by inflammatory cytokines. Bottom: Total integrated intensity of adhesion molecules expressed in the presence of anti-inflammatory compounds (blue - SB202190; red - SB203580).


Technical Specifications

Light source Proprietary solid state illumination, White, 460/20 nm and 625/20 nm excitation
Detector 1.25 megapixel, 12-bit high sensitivity CCD camera
Emission Brightfield; Green 541/108 nm; Red 713/123 nm
Objective Single 4X objective
Autofocus Proprietary laser scanning autofocus
Resolution 1.9 μm x 1.9 μm pixel size
Data acquisition and analysis software SoftMax® Pro Software MiniMax Imaging Edition
Imaging Speed* Acquisition Acquisition + Analysis
1 color - 96 wells 3:40 6:30
2 color - 96 wells 3:40 6:30
Specimen carriers ANSI/SBS-conformant microplates,
96 and 384 wells
Dimensions (cm) 39.2 cm W x 19.5 cm H x 60.6 cm D (SpectraMax® MiniMax™ 300 Imaging Cytometer) 
39.2 W x 44.0 H x 60.6 L
(with base system)

* Using single site acquisition with 10 ms exposure time.

Related Video

SpectraMax i3x Multi-Mode Microplate Reader System

Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video.


Related Collateral

Data Sheet



SpectraMax MiniMax300细胞成像系统



Related Webinar

Cell-based Assays Using a Novel Plate Reader Platform with Imaging Cytometry

Visit @ the Cell Counter for our monthly features.

The SpectraMax MiniMax 300 Imaging Cytometer is a field-upgradable option to the SpectraMax i3 Multi-Mode Detection Platform. It can be purchased together with the SpectraMax i3 base system or added separately should your application needs expand in the future.


Part Number

SpectraMax MiniMax 300 Imaging Cytometer
SpectraMax MiniMax 300 Desktop Computer
SpectraMax MiniMax 300 22" Monitor
SpectraMax i3x Multi-Mode Microplate Reader