SpectraMax M Series Multi-Mode Microplate Readers
Configurable readers with triple-mode cuvette ports, validation tools, and compliance software
Dual monochromators increase performance and assay flexibility
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station. With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.
Features
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Patented optimization
The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays.
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IQ/OQ/PM Services
Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.
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Validation tools
Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data.
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Compliance software
SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records.
Proven GxP solutions to assure data integrity and compliance
Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.
- Best-in-class microplate readers and washers support all your assay needs
- IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
- Software installation services verify and document that required components are installed to operational specifications
- Software validation service supports FDA 21 CFR Part 11 guidelines
- Validation plates test the performance of your microplate reader using traceable materials for reliable results
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Application Note
Optimized HTRF settings for SpectraMax multi-mode microplate readers
Optimized HTRF settings for SpectraMax multi-mode microplate readers
HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers.
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Application Note
Faster ELISA results with CatchPoint SimpleStep ELISA kits
Faster ELISA results with CatchPoint SimpleStep ELISA kits
CatchPoint SimpleStep ELISA kits give users a 90-minute ELISA workflow, a nearly two-thirds reduction in time compared to conventional ELISAs.
Applications of SpectraMax M Series Multi-Mode Microplate Readers
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Absorbance
Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.
Absorbance
Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
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ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
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Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.
Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.
Luminescence
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.
Luminescence
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.
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SNP Genotyping
Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. SNP genotyping has proven very useful in identifying disease-related mutations in various species, and as a result many techniques for SNP detection have been developed.
SNP Genotyping
Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. SNP genotyping has proven very useful in identifying disease-related mutations in various species, and as a result many techniques for SNP detection have been developed.
TRF, TR-FRET & HTRF
Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.
TRF, TR-FRET & HTRF
Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.
Specifications & Options of SpectraMax M Series Multi-Mode Microplate Readers
Resources of SpectraMax M Series Multi-Mode Microplate Readers
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Application Note
Optimized HTRF settings for SpectraMax multi-mode microplate readers
Optimized HTRF settings for SpectraMax multi-mode microplate readers
HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers.
Flyer
Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay
Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay
ATP standards ranging from 1 μM to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). 50 μL of standard was pipetted into the wells of a white…
eBook
Software compliance for GMP/GLP labs
Software compliance for GMP/GLP labs
Become 21 CFR Part 11 compliant with SoftMax Pro 7.1.1 GxP Software.
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Application Note
Faster ELISA results with CatchPoint SimpleStep ELISA kits
Faster ELISA results with CatchPoint SimpleStep ELISA kits
CatchPoint SimpleStep ELISA kits give users a 90-minute ELISA workflow, a nearly two-thirds reduction in time compared to conventional ELISAs.
Citations
Novel Interleukin-6 Inducible Gene PDZ-Binding Kinase Promotes Tumor Growth of Multiple Myeloma Cells
Novel Interleukin-6 Inducible Gene PDZ-Binding Kinase Promotes Tumor Growth of Multiple Myeloma Cells
Multiple myeloma (MM) remains an intractable hematological malignancy, despite recent advances in anti-MM drugs. Here, we show that role of PDZ binding kinase (PBK) in MM tumor growt…
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Citations
Assessment of genetically engineered events in heat-treated and non-treated samples using droplet digital PCR and real-time quantitative PCR
Assessment of genetically engineered events in heat-treated and non-treated samples using droplet digital PCR and real-time quantitative PCR
Identification and quantification of genetically engineered (GE) events is routinely utilized due to established regulations and labeling requirements of many countries. Food process…
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eBook
From ELISA to epigenetics with HTRF
From ELISA to epigenetics with HTRF
HTRF® is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Assays encompass the areas of G protein-coupled receptors (GPCR), kinases and cel…
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Application Note
Comparing performance of SpectraMax absorbance microplate readers using an endotoxin assay
Comparing performance of SpectraMax absorbance microplate readers using an endotoxin assay
Control Standard Endotoxin (CSE) was reconstituted with the volume of LAL reagent water (LRW) indicated on the Certificate of Analysis included with the kit to yield a solution of 100…
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Citations
Antibiotic ivermectin selectively induces apoptosis in chronic myeloid leukemia through inducing mitochondrial dysfunction and oxidative stress
Antibiotic ivermectin selectively induces apoptosis in chronic myeloid leukemia through inducing mitochondrial dysfunction and oxidative stress
Mitochondria has been a promising target in blood cancer given their unique dependencies on mitochondrial functions compared to normal hematopoietic cells. In line with this concept,…
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Application Note
HTRF cAMP dynamic 2 and IP-One assays on the SpectraMax M5e reader
HTRF cAMP dynamic 2 and IP-One assays on the SpectraMax M5e reader
HTRF is a versatile technology developed by Cisbio for detecting molecular interactions between biomolecules.1 Combining the features of fluorescence resonance energy transfer (FRET)…
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eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
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eBook
Power of SpectraMax M
Power of SpectraMax M
For over 13 years, scientists have relied on the industry standard, the SpectraMax® M Series Multi-Mode
Microplate Readers, for their consistent performance, reliability and du… -
Application Note
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
This application note describes how to use the NanoOrange® Protein Quantitation Kit from Life Technologies in SpectraMax® microplate readers with the fluorescence detection mode and…
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Application Note
MycoAlert Mycoplasma Detection Assays on Molecular Devices Microplate Readers
MycoAlert Mycoplasma Detection Assays on Molecular Devices Microplate Readers
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of…
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Application Note
IMAP FP kinase assays on the SpectraMax M5 Multi-Mode Microplate Reader
IMAP FP kinase assays on the SpectraMax M5 Multi-Mode Microplate Reader
Protein kinases are central to the regulation of many cellular processes. In recent years they have emerged as one of the most important classes of drug targets for cancer and many…
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Application Note
EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
Cell viability assays are often performed to assess the effects of a variety of treatments, including drug candidates, pathway activators and inhibitors, and reporter genes. One of the…
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Application Note
Protein quantitation with the EMax Plus Microplate Reader
Protein quantitation with the EMax Plus Microplate Reader
Endpoint readers are prolific in the laboratory since absorbance has become the detection of choice for many applications. Examples include ELISAs for quantitation of cytokines and…
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Application Note
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth. The CyQUANT Cell Proliferation…
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Application Note
Noninvasive measurement of fluorescent proteins in live cells
Noninvasive measurement of fluorescent proteins in live cells
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria was the…
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Application Note
Quant-iT PicoGreen dsDNA assay with SpectraMax Microplate Readers
Quant-iT PicoGreen dsDNA assay with SpectraMax Microplate Readers
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about…
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Application Note
IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers
IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers
Cyclic nucleotide phosphodiesterases (PDEs) are a group of enzymes that degrade the phosphodiester bond of cAMP and cGMP, second messengers that are involved in a variety of biological…
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Application Note
HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
HTRF is a versatile technology developed by Cisbio Bioassays for detecting biomolecular interactions1. It combines fluorescence resonance energy transfer (FRET) technology with time-…
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Application Note
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA Boosters
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA Boosters
Traditional ELISA (enzyme-linked immunosorbent assay) methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance…
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Application Note
Detect SNPs with KASP genotyping technology on SpectraMax microplate readers
Detect SNPs with KASP genotyping technology on SpectraMax microplate readers
Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. The many methods available for genotyping enable researchers to…
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Application Note
Multitask kinetic measurements of IPTG’s effects on protein expression and cell growth
Multitask kinetic measurements of IPTG’s effects on protein expression and cell growth
Measuring several different signal outputs simultaneously over time is especially useful for studying a protein or compound’s effect on cellular growth or gene expression. In this…
Scientific Poster
High -Throughput Multiplexed Inflammation Assay Using Primary Cells
High -Throughput Multiplexed Inflammation Assay Using Primary Cells
Learn how we performed a multiplexed, primary human cell-based assay to fluorometrically measure inflammation markers using our SpectraMax microplate readers in this poster.
Brochure
SpectraMax M Series Multi-Mode Microplate Readers
SpectraMax M Series Multi-Mode Microplate Readers
Learn more about the SpectraMax M Series Microplate readers. They contain a wide range of high-performance capabilities ideal for life science research & drug discovery screening…
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Brochure
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. O…
SpectraMax M5e Multi-Mode Microplate Reader
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Citations
Novel Interleukin-6 Inducible Gene PDZ-Binding Kinase Promotes Tumor Growth of Multiple Myeloma Cells
Multiple myeloma (MM) remains an intractable hematological malignancy, despite recent advances in anti-MM drugs. Here, we show that role of PDZ bin…
-
Citations
Assessment of genetically engineered events in heat-treated and non-treated samples using droplet digital PCR and real-time quantitative PCR
Identification and quantification of genetically engineered (GE) events is routinely utilized due to established regulations and labeling requireme…
-
Citations
Antibiotic ivermectin selectively induces apoptosis in chronic myeloid leukemia through inducing mitochondrial dysfunction and oxidative stress
Mitochondria has been a promising target in blood cancer given their unique dependencies on mitochondrial functions compared to normal hematopoieti…
SpectraMax® M Series Multi-Mode Microplate Readers
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Product |
Part Number |
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SpectraMax M2 Multi-Mode Microplate Reader |
M2 |
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SpectraMax M2E Multi-Mode Microplate Reader |
M2E |
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SpectraMax M3 Multi-Mode Microplate Reader |
M3 |
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SpectraMax M4 Multi-Mode Microplate Reader |
M4 |
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SpectraMax M5 Multi-Mode Microplate Reader |
M5 |
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SpectraMax M5E Multi-Mode Microplate Reader |
M5E |
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SpectraTest ABS2 Absorbance Validation Plate |
0200-6191 |
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SpectraTest FL1 Fluorescence Validation Plate |
0200-5060 |
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SpectraTest LM1 Luminescence Validation Plate |
0200-6186 |
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SpectraDrop™ Micro-Volume Starter Kit |
0200-6262 |
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SpectraDrop™ Micro-Volume HTS Kit |
0200-6267 |
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StakMax Microplate Handling Stacker |
StakMax |
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Reader Shelf |
9000-0756 |
Related Products & Services of SpectraMax M Series Multi-Mode Microplate Readers
Featured Applications
Absorbance
Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to…
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the…
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein,…
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to…
Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in…
Luminescence
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence…
SNP Genotyping
Genotyping is a process for analyzing genetic differences among individuals by examining their DNA…
TRF, TR-FRET & HTRF
Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide…
Customer Breakthrough
SUCCESS STORY
American Heritage School uses the SpectraMax M5e reader to conduct scientific research
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
Maximize flexibility in application workflow and experimental design
Contact our specialist today to discuss how to implement a solution that works for you