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Dual monochromators increase performance and assay flexibility

The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station. With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.

  • Assay flexibility Icon

    Assay flexibility

    The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers.

  • Accuracy Icon

    Better absorbance accuracy

    Measure sample depth with no temperature dependency using the patented PathCheck® Sensor technology. It automatically normalizes absorbance readings to 1 cm, eliminating the need for standard curves.

  • Comprehensive Reader Validation Icon

    Increased throughput

    Integrate easily with our StakMax® Microplate Stacker for walk-away automation. The stacker can hold up to 50 plates and can be configured to include a barcode reader.

SpectraMax m5e Multi Mode Microplate Reader

SpectraMax M5e Multi-Mode Microplate Reader

Features

  • Expandable Icon

    Patented optimization

    The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays.

  • Protocols Icon

    IQ/OQ/PM Services

    Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.

  • Validation Icon

    Validation tools

    Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data.

  • Compliance Software Icon

    Compliance software

    SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records.

Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational specifications
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs

 

Applications of SpectraMax M Series Multi-Mode Microplate Readers

  • Absorbance

    Absorbance

    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    Cell Viability

    Cell Viability

    Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.

    Read Application Note 

  • ELISA

    Elisa

    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

    Fluorescence

    Fluorescence

    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  

  • Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

    Learn more  

    Fluorescent Protein Detection

    Fluorescent Protein Detection

    Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.

    Read Application Note 

  • Luminescence

    Luminescence

    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

    Learn more  

    SNP genotyping

    SNP Genotyping

    Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. SNP genotyping has proven very useful in identifying disease-related mutations in various species, and as a result many techniques for SNP detection have been developed.

  • TRF, TR-FRET & HTRF

    Time-resolved

    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

    Learn more  

Specifications & Options of SpectraMax M Series Multi-Mode Microplate Readers

 

*Using lowest settings and speed read when available.

Resources of SpectraMax M Series Multi-Mode Microplate Readers

Presentations
Videos & Webinars
SpectraMax m5e Multi Mode Microplate Reader

SpectraMax M5e Multi-Mode Microplate Reader

  • Citation
    Dated: Jan 15, 2012
    Publication Name: European Journal of Pharmacology

    Protective effects of baicalein against rotenone-induced neurotoxicity in PC12 cells and isolated rat brain mitochondria

    Baicalein is one of the major flavonoids obtained from the Scutellaria root. Previous pharmacological studies found that baicalein had neuroprotective effects in animal models of Parkinson's disease. The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria.… View more

    Baicalein is one of the major flavonoids obtained from the Scutellaria root. Previous pharmacological studies found that baicalein had neuroprotective effects in animal models of Parkinson's disease. The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria. Firstly, we investigated the effects of baicalein on rotenone-induced toxicity in PC12 cells. The results showed that baicalein suppressed rotenone-induced apoptosis, and inhibited the accumulation of reactive oxidant species, ATP deficiency, mitochondrial membrane potential dissipation, and caspase-3/7 activation in a concentration-dependent manner, indicating that baicalein likely improved mitochondrial function. Furthermore, we used isolated rat brain mitochondria to evaluate the effect of baicalein. Treatment with baicalein prevented rotenone-induced reactive oxidant species production, ATP deficiency and mitochondrial swelling in isolated brain mitochondria. Interestingly, exposure of isolated mitochondria to baicalein promoted mitochondrial active respiration. These results suggest that baicalein may be a mitochondria-targeted antioxidant and exerts neuroprotective effects on rotenone-induced neurotoxicity. This study supports our previous research that baicalein possesses neuroprotective activity in vivo and it is worthy of further study.

    Contributors: Xiao-xiu Li, Guo-rong He, Xin Mu Bei Xu, Shuo Tian Xin Yu, Fan-rui Meng, Zhao-hong Xuan, Guan-huaDu  
    Go to article

  • Citation
    Dated: Jun 30, 2011
    Publication Name: Molecular and Cellular Biochemistry

    Chronic hyperhomocysteinemia induces oxidative damage in the rat lung

    Tissue accumulation of homocysteine occurs in classical homocystinuria, a metabolic disease characterized biochemically by cystathionine β-synthase deficiency. Vascular manifestations such as myocardial infarction, cerebral thrombosis, hepatic steatosis, and pulmonary embolism are common in this disease and poorly understood. In this study, we… View more

    Tissue accumulation of homocysteine occurs in classical homocystinuria, a metabolic disease characterized biochemically by cystathionine β-synthase deficiency. Vascular manifestations such as myocardial infarction, cerebral thrombosis, hepatic steatosis, and pulmonary embolism are common in this disease and poorly understood. In this study, we investigated the effect of chronic hyperhomocysteinemia on some parameters of oxidative stress (thiobarbituric acid-reactive substances, protein carbonyl content, 2′,7′-dichlorofluorescein fluorescence assay, and total radical-trapping antioxidant potent) and activities of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in the rat lung. Reduced glutathione content and glucose 6-phosphate dehydrogenase activity, as well as nitrite levels, were also evaluated. Wistar rats received daily subcutaneous injections of Hcy (0.3–0.6 μmol/g body weight) from the 6th to the 28th days-of-age and the control group received saline. One and 12 h after the last injection, rats were killed and the lungs collected. Hyperhomocysteinemia increased lipid peroxidation and oxidative damage to protein, and disrupted antioxidant defenses (enzymatic and non-enzymatic) in the lung of rats, characterizing a reliable oxidative stress. In contrast, this amino acid did not alter nitrite levels. Our findings showed a consistent profile of oxidative stress in the lung of rats, elicited by homocysteine, which could explain, at least in part, the mechanisms involved in the lung damage that is present in some homocystinuric patients.

    Contributors: Aline A. da Cunha, Andréa G. K. Ferreira, Maira J. da Cunha, Carolina D. Pederzolli, Débora L. Becker, Juliana G. Coelho, Carlos S. Dutra-Filho & Angela T. S. Wyse  
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  • Citation
    Dated: Feb 02, 2009
    Publication Name: Translational Research

    Impaired antioxidant activity of high-density lipoprotein in chronic kidney disease

    Chronic kidney disease (CKD) is associated with accelerated atherosclerosis and increased mortality from cardiovascular disease. CKD results in oxidative stress, inflammation, and high-density lipoprotein (HDL) deficiency, which work in concert to promote atherosclerosis. Normal HDL confers protection against atherosclerosis by inhibiting the… View more

    Chronic kidney disease (CKD) is associated with accelerated atherosclerosis and increased mortality from cardiovascular disease. CKD results in oxidative stress, inflammation, and high-density lipoprotein (HDL) deficiency, which work in concert to promote atherosclerosis. Normal HDL confers protection against atherosclerosis by inhibiting the oxidation of lipids and lipoproteins and by retrieving surplus cholesterol and phospholipids from lipid-laden cells in the artery wall for disposal in the liver (reverse cholesterol transport).

    Contributors: Hamid Moradi, Madeleine V.Pahl, Reza, Elahimehr, Nosratola D.Vaziri  
    Go to article

SpectraMax® M Series Multi-Mode Microplate Readers

Product

Part Number

SpectraMax M2 Multi-Mode Microplate Reader

M2

SpectraMax M2E Multi-Mode Microplate Reader

M2E

SpectraMax M3 Multi-Mode Microplate Reader

M3

SpectraMax M4 Multi-Mode Microplate Reader

M4

SpectraMax M5 Multi-Mode Microplate Reader

M5

SpectraMax M5E Multi-Mode Microplate Reader

M5E

SpectraTest ABS2 Absorbance Validation Plate

0200-6191

SpectraTest FL1 Fluorescence Validation Plate

0200-5060

SpectraTest LM1 Luminescence Validation Plate

0200-6186

SpectraDrop™ Micro-Volume Starter Kit

0200-6262

SpectraDrop™ Micro-Volume HTS Kit

0200-6267

StakMax Microplate Handling Stacker

StakMax

Reader Shelf

9000-0756

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