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Dual monochromators increase performance and assay flexibility

The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station. With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.

  • Assay flexibility

    Assay flexibility

    The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers.

  • Patented PathCheck Pathlength Measurement Technology

    Better absorbance accuracy

    Measure sample depth with no temperature dependency using the patented PathCheck® Sensor technology. It automatically normalizes absorbance readings to 1 cm, eliminating the need for standard curves.

  • Comprehensive Reader Validation

    Increased throughput

    Integrate easily with our StakMax® Microplate Stacker for walk-away automation. The stacker can hold up to 50 plates and can be configured to include a barcode reader.

SpectraMax m5e Multi Mode Microplate Reader

SpectraMax M5e Multi-Mode Microplate Reader


  • Patented optimization

    The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays.

  • IQ/OQ/PM Services

    Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.

  • Validation tools

    Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data.

  • Compliance software

    SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records.

Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational specifications
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs


Applications of SpectraMax M Series Multi-Mode Microplate Readers

  • Absorbance


    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

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    Cell Viability

    Cell Viability

    Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.

    Read Application Note 



    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

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    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

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  • Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

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    Fluorescent Protein Detection

    Fluorescent Protein Detection

    Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.

    Read Application Note 

  • Luminescence


    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

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    SNP Genotyping

    SNP Genotyping

    Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. SNP genotyping has proven very useful in identifying disease-related mutations in various species, and as a result many techniques for SNP detection have been developed.

    Read Application Note 



    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

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Specifications & Options of SpectraMax M Series Multi-Mode Microplate Readers


*Using lowest settings and speed read when available.

Resources of SpectraMax M Series Multi-Mode Microplate Readers

Videos & Webinars
SpectraMax m5e Multi Mode Microplate Reader

SpectraMax M5e Multi-Mode Microplate Reader

SpectraMax® M Series Multi-Mode Microplate Readers


Part Number

SpectraMax M2 Multi-Mode Microplate Reader


SpectraMax M2E Multi-Mode Microplate Reader


SpectraMax M3 Multi-Mode Microplate Reader


SpectraMax M4 Multi-Mode Microplate Reader


SpectraMax M5 Multi-Mode Microplate Reader


SpectraMax M5E Multi-Mode Microplate Reader


SpectraTest ABS2 Absorbance Validation Plate


SpectraTest FL1 Fluorescence Validation Plate


SpectraTest LM1 Luminescence Validation Plate


SpectraDrop™ Micro-Volume Starter Kit


SpectraDrop™ Micro-Volume HTS Kit


StakMax Microplate Handling Stacker


Reader Shelf


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