Cell line development solutions with automated clone screening

The ClonePix 2 Mammalian Colony Picker is a fully automated system for the selection of high-value clones used in antibody discovery and cell line development. Hybridoma, CHO cells, and other cell types are imaged and selected based on user-defined parameters. Plate handling, barcode reading, and picking are all fully integrated. All data, including images, are saved for downstream analysis. The picker increases the probability of finding optimally produced cell lines and significantly reduces time and labor.

  • Efficient

    Screen more clones in less time

    ClonePix 2 picker is 10x faster than labor-intensive limiting dilution and FACS. Our sophisticated software and integrated robotics enables a picking speed of > 10,000 clones per day.

  • Select

    Select cells with desirable attributes

    Easily screen and select clones based on protein productivity, antigen-specificity, cell viability, and expression levels of tagged recombinant proteins.

  • Accuracy

    Pick colonies with accuracy

    Picking accuracy < 1 mm. Robotic picking reduces risk of colony disturbance. Images of picked clones are stored with data.

ClonePix 2 System

ClonePix 2

Features

  • Multiple detection methods

    White light identifies and measures clone morphology, size and proximity. Fluorescence indicates expression level and/or specificity. Up to five fluorescent filters are available for multiplexing.

  • Sterility maintenance

    A host of sterility features and options including a UV light process for sanitizing the interior of the instrument, as well as pin washing and halogen drying are standard.

  • Integrated plate storage

    Includes two storage stacks for source and destination plates, each with a capacity of 10 plates.

  • Discrete colony formation

    Semi-solid CloneMediaTM encourages single cells to grow into discreet colonies, and allows for ease of plating. The media allows for a higher density of clones to be screened.

  • Animal-free media and reagents

    Chemically defined and animal-free, CloneMedia cell-culture media, is optimized to increase productivity and aid in visualizing secreted antibodies when used with the CloneDetectTM detection agent.

  • Custom automation options*

    The Advanced Workflow Engineering Solutions Team can customize the monoclonality system and offer added services such as integrated verification of monoclonality.

*Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.
 

Applications of ClonePix 2 Mammalian Colony Picker

  • Hybridoma Screening

    Hybridoma Screening

    Antibody discovery typically refers to the screening and identification of monoclonal antibodies (mAbs) that target a specific epitope for the diagnosis and treatment of diseases. A common approach to generating monoclonal antibodies involves the fusion of a pre-mitotic cancer cell with a post-mitotic and terminal antibody-expressing B-cell from the spleen. The resulting fused cell is called a hybridoma and has the advantage of producing mAbs while dividing to regenerate itself. Screening hybridomas for binding specificity or productivity can be automated using the ClonePix 2 System.

    Read Application Note 

  • Cell Line Development for Recombinant Proteins

    Cell Line Development

    Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.

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  • Monoclonality Assurance System

    Monoclonality Assurance

    Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.

    Read Application Note 

  • Clone Productivity Screening

    Clone Productivity Screening

    An important component in identifying high-value clones is determining productivity of single cell-derived colonies. Screening for productivity using traditional approaches is laborious and time-consuming, generally consisting of a multi-step process that involves isolating single cells from limiting dilution followed by assessment of titer using ELISA. The ClonePix 2 System combines single-cell isolation and productivity screening into a single step, resulting in dramatically shorter screening times and increased number of candidates.  

  • Cell Surface Expression Screening

    Cell Surface Expression Screening

    Many proteins that express to the surface of cells are targets for the discovery and development of biopharmaceuticals. For instance, G-protein coupled receptors (GPCRs) are the largest class of cell-surface proteins and are targets for almost 40% of existing drugs. Discovery and selection of high-value cell surface clones from a transfected pool of cells can be challenging. The ClonePix 2 System represents an automated method of screening large populations of cells that increases the probability of finding rare high-affinity binder or high producer.

    Read Application Note 

  • Antigen-Specific Screening

    Antigen-Specific Screening

    Antibody discovery typically refers to the screening and identification of specific antibodies that target an antigen molecule for the diagnosis and treatment of diseases. The specificity of the antibody is based on its ability to bind the epitope, a unique region on the antigen molecule. Therapeutic antibodies are typically monoclonal, single cell-derived and target a unique epitope region on the antigen. The ClonePix 2 System automates screening and rapid detection of antigen-specific clones from a heterogenous population of cells.

Specifications & Options of ClonePix 2 Mammalian Colony Picker

Resources of ClonePix 2 Mammalian Colony Picker

Videos and Demos

Identification and Selection of GPCR Cell Lines with ClonePix 2

Identification and Selection of GPCR Cell Lines with ClonePix 2

ClonePix 2 System

ClonePix 2

Related Products & Services of ClonePix 2 Mammalian Colony Picker

Featured Applications

Customer Breakthrough

The Fred Hutch Cancer Research Center uses ClonePix 2 System to Screen & Isolate Positive Hybridoma Clones
The Fred Hutch Cancer Research Center uses ClonePix 2 System to Screen & Isolate Positive Hybridoma Clones

SUCCESS STORY

The Fred Hutch Cancer Research Center uses the ClonePix 2 system to efficiently screen and isolate positive hybridoma clones

The Fred Hutch Cancer Research Center uses ClonePix 2 system to efficiently screen and isolate positive hybridoma clones

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.