Culturing brain organoids

Culturing brain organoids

Culturing brain organoids is notoriously difficult, even compared to other organoid types. In addition to requiring precise, consistent handling, the culture process frequently exceeds 100 days before the organoids fully mature. During this time, they often require daily media exchanges and to be kept in constant motion. This is because brain organoids are highly metabolically active and consume large quantities of nutrients during development. Without this motion, parts of the organoid may not get adequate nourishment.

Automating the brain organoid workflow reduces hands-on time, minimizes human error, and improves consistency—resulting in more reliable, reproducible assays downstream. The addition of a rocking incubator to the CellXpress.ai® Automated Cell Culture System now automates long-term brain organoid workflows–reducing manual hands-on time up to 90%.