Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Learn how to use both imaging and calcium oscillation analysis to develop profiles of compounds in iPSC-derived cardiomyocytes such as hERG blockers, ß-adrenergic agonists, and environmental toxins. iPSC-derived neuronal cultures were evaluated with neuromodulators as well as environmental toxins.
What you will find in this ebook
There is a critical need for more relevant models to identify therapeutic effects and adverse responses to compounds earlier in the drug discovery process. Profiling the effects of environmental hazards in a higher throughput fashion is extremely important. The development of human induced pluripotent stem cells (iPSC) has made it possible to develop new higher throughput screening assays to study compound effects upon iPSC cardiomyocytes and neuronal cultures.
Cytotoxicity and mitochondrial integrity studies using the ImageXpress® Micro Confocal High-Content Imaging System with both 2D and spheroid cultures provide an accurate platform for data analysis. Spontaneous calcium oscillation acquired with FLIPR® Calcium 6 Assay Kit and the FLIPR High-Throughput Cellular Screening System from both iPSC cardiomyocytes and neuronal cultures can be paired with cytotoxicity and mitochondrial integrity data from the ImageXpress Micro Confocal system for comprehensive phenotypic analysis and bioactivity profiling of both pharmaceutical and environmental compounds.
Includes data acquired with:
- FLIPR High-Throughput Cellular Screening System
- ImageXpress® Micro Confocal High-Content Imaging System
- FlexStation® 3 Multi-Mode Microplate Reader
- FLIPR Calcium Assay Kits, EarlyTox™ Cardiotoxicity Kit, more
Identify therapeutic effects and adverse responses to compounds earlier in the drug discovery process
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Learn more about the systems behind the methods:
FLIPR Penta High-Throughput Cellular Screening System
High-throughput kinetic screening for toxicology and lead compound identification
Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per second and quickly cherry pick events such as Early Afterdepolarization-like events (EAD-like events), fibrillation, and irregular beating.
ImageXpress Micro Confocal system
High-content confocal imaging solution with proprietary spinning disk technology and water objective options
- Ideal for 3D organoid and spheroid imaging
- Proprietary AgileOptix™ spinning disk technology
- QuickID reduces image acquisition time and data storage requirements
- Water immersion objectives enhance resolution, sensitivity and throughput of complex 3D assays
- Lasers and deep tissue confocal disk provide deeper penetration into 3D samples
- 3D volumetric analysis
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