SENS Research Foundation uses the ImageXpress Micro Widefield system to speed up cancer research 20 times

"The problem that we encountered was that, while the image quality was good for all instruments, most couldn’t achieve the throughput desired. With the ImageXpress Micro, however, we found good image quality and automation that enabled, hands-down, the fastest assays in terms of data acquisition and analysis "

SENS Research Foundation

Harold Silva, Ph.D.

Research Scientist, OncoSENS Lead

ImageXpress Micro 4 High-Content Imaging System

MetaXpress High-Content Image Acquisition and Analysis Software

The Challenge

The Oncology team at the SENS Research Foundation seeks to uncover the role of telomere lengthening in cancer development. The Alternative Lengthening of Telomeres (ALT) pathway accounts for about 10-15% of cancers, yet no ALT-specific anticancer therapeutics currently exist.

Little is known about ALT and few labs study the pathway. Existing protocols were time consuming, and methodologies varied making it difficult to collect statistically-relevant data. The lack of robust and functional ALT-relevant assays had slowed progress.

Harolado Silva

Sens Research Foundation

The Solution

To better characterize ALT activity, a high-content assay utilizing the ImageXpress® Micro system was established and validated to identify ALT-positive cells. This high-content imaging workflow is not only ~20 times faster than the previous protocol, but also provides a standardized approach for assay development in this field.

The Result

The addition of the ImageXpress Micro system has allowed the team to be more ambitious. Before the HCS assay, only a few 8-well slides were analyzed in a week. Afterwards, analysis of 100 96-well plates was attainable in the same timeframe.

"Now I can screen a whole library of genes and find the group of genes that are responsible for the ALT pathway."

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