Multiplex immunoassays

APPLICATION NOTE

Measure Two Biomarkers in One Well with High-Sensitivity Multiplex TR-FRET

Discover how the SpectraMax® iD5e reader and Bright-Dtech™ technology enable simultaneous IgG and IgM detection with low limits of detection, reduced sample consumption, and streamlined, no-wash workflows.

App Note on High-Sensitivity Multiplex TR-FRET

Highly sensitive multiplex TR-FRET assay on the SpectraMax iD5e Multi-Mode Microplate Reader

Multiplex immunoassays can help researchers maximize valuable samples while improving laboratory efficiency. In this application note, learn how the SpectraMax® iD5e Multi-Mode Microplate Reader was used with Poly-Dtech's Bright-Dtech™ Multiplex TR-FRET technology to simultaneously quantify IgG and IgM in a single well. The homogeneous, no-wash assay delivers excellent sensitivity, low background fluorescence, and a broad dynamic range, while reducing reagent use, plate consumption, and assay preparation time. You'll also see how automated data acquisition and analysis in SoftMax® Pro Software help simplify workflows and ensure consistent, high-quality results.

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Product family
Microplate Reader
Product primary application
AquaMax Microplate Washer; FlexStation 3 Multi-Mode Microplate Reader; FlexStation 3 Pipet Tips; Gemini XPS and EM Microplate Readers; MultiWash+ Microplate Washer; SoftMax Pro Software; SpectraDrop Micro-Volume Microplate; SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax Injector Cartridge with SmartInject Technology; SpectraMax L Microplate Reader;SpectraMax M Series Multi-Mode Microplate Readers; SpectraMax Mini Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer; SpectraMax Paradigm Multi-Mode Microplate Reader; SpectraMax QuickDrop Micro-Volume Spectrophotometer; SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax iD3 and iD5 Multi-Mode Microplate Readers; StakMax Microplate Handling System; Western Blot Cartridge; Biomarker Discovery; Protein-Detection-Quantitation-and-Analysis; TRF-TR-FRET-HTRF; Fluorescence
CMP
701Nr000012KcCMIA0
Title
Measure Two Biomarkers in One Well with High-Sensitivity Multiplex TR-FRET
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App Note on High-Sensitivity Multiplex TR-FRET
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