What is the whole cell patch-clamp technique?

Cell Patch-Clamp Technique illustration

 

The whole cell patch-clamp technique involves a glass micropipette forming a tight gigaohm (GΩ) seal with the cell membrane. A patch of membrane is subsequently ruptured by mild suction so that the glass micropipette provides a low-resistance access to the whole cell, thereby allowing the investigator to control the transmembrane voltage.

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