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What is the two electrode voltage-clamp (TEVC) method?

The TEVC method is a patch-clamp electrophysiology method for injecting current into a cell with one electrode and recording the change in voltage with the other electrode.  

  • Electrophysiology patch-clamp method for large cells such as oocytes
  • Set Vcmd to force injection of current into a cell with one microelectrode (ME2)
  • Record change in membrane potential with the other microelectrode (ME1)

Circuit diagram for a two electrode voltage-clamp (TEVC) method

A1 = Unity gain amplifier
A2 = High gain differential amplifier
Vcmd = Command voltage
Vm= Membrane Potential
Cm= Capacitance of membrane
m = gain
ME1 = Measuring electrode 1
ME2= Measuring electrode 2
Rp1 = Resistance of pipette 1
Rp2 = Resistance of pipette 2
Rm= Resistance of membran

For more comprehensive information, please download our Axon Guide.

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