Pulmonary (Lung) Organoids

Automation of 3D lung organoid culture and experimental protocols

Lung organoids can be cultured and monitored via automated workflows. The automated integrated system below includes ImageXpress Confocal HT.ai system and analysis software, automated incubator, Biomek liquid handler, and a robotic device. Machine-learning-based image analysis, can track the growth of diameter and area, density and number of objects. Advanced image analysis allows 3D reconstitution and complex analysis of organoids, including cell morphology, viability, and differentiation markers.

Experimental protocol for lung organoids

1. 2D pre-culture - 3D lung organoids were derived from primary human lung epithelial cells and expanded for two weeks in 2D.
2. Developing 3D organoids - Then the cells were seeded in 90% Matrigel domes using reagents. Cells formed 3D organoids and were fed every second day for two weeks using seeding media, then differentiated for another six weeks.
3. Organoid culture - Organoids were grown in different plate formats and then treated with compounds or stained with various markers for morphology and viability assessment.
4. Monitoring organoid growth & development - Organoid growth and development were monitored in Matrigel using transmitted light imaging or using Hoechst stain that allowed evaluation of organoids size, volume, and complexity.
5. Confocal imaging and 3D/AI analysis - Confocal imaging in combination with 3D analysis allowed quantitative characterization of cellular content as well as count and measurement cells with different phenotypes within organoids (cell count, live-dead assessment, cell scoring for specific markers, others). The model can be used for toxicity evaluation of pharmaceutical drugs and other compounds.

Our lab automation for high-throughput, high-content screening include scientists and engineers who can customize our instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. From incubators, liquid handlers, and robotics to customized software and hardware—and with over 35 years of experience in the life science industry—you can count on us to deliver quality products and provide worldwide support.