Pulmonary (Lung) Organoids

Lung organoid cultures are 3D microtissue models recapitulating the morphological and functional characteristics of the airway. They can be formed primary human lung epithelial cells in ECM with growth factors. The cells can self-assemble into the multi-lineage lung epithelium comprising different cell populations.

Lung organoids display characteristic features of the human airway, such as mucus secretion, ciliary beating, and regeneration. This biological relevance enables the study of repair/regeneration mechanisms in lung injury and phenotypic changes in pulmonary diseases. Lung organoids also can be used for toxicity assessment or drug testing.

Because lung organoids are hollow, with lumen and cavities inside, they are easily penetrated by light. This makes them compatible with 3D biological assays and suitable for confocal imaging, allowing quantitative characterization of cellular content, live-dead assessment, and cell scoring for specific markers.

Lung organoids can be cultured and monitored via automated workflows. Machine-learning-based image analysis, can track the growth of diameter and area, density and number of objects. Advanced image analysis allows 3D reconstitution and complex analysis of organoids, including cell morphology, viability, and differentiation markers.