HumSilencer Technology

Electric hum - A common patch clamp problem

 

50/60 Hz line-frequency noise, also known as electrical hum, is the most common source of background noise in patch-clamp electrophysiology experiments. Originating from alternating current (AC) of the electrical mains and delivered via power outlets, this noise can often overwhelm biological signals of interest, making sensitive current or voltage measurements nearly impossible.

Typical troubleshooting can be time-consuming and only partially effective as researchers need to identify and either eliminate or shield noise source. While notch or off-line filtering methods can be employed, often these filter-based methods do not fully remove electrical noise, and in some situations can actually distort biological signals and impair data accuracy.

Electrical hum overwhelming a single-channel recording

Electrical hum overwhelming a single-channel recording of the principal subunit of olfactory cyclic nucleotide-gated channel (CNCA2). Data courtesy of Tsung-Yu Chen, Ph.D., University of California, Davis.

HumSilencer eliminates electrical hum without causing signal distortion

The HumSilencer™ technology available in Axon™ Digidata® 1550B Low Noise Data Acquisition System is a filter-free, adaptive technology that learns and removes local line-frequency noise patterns from incoming signal in less than one second.

 

Amplitude at Digitizer's Analog Input

Learns and adapts to changing noise profiles

HumSilencer enabled, signal measurements of CNCA2

 

 

Patch and Record Cellular Network Noise-Free

Equipped with up to four analog HumSilencer inputs, the Digidata 1550B System enables you to record multiple cells at once for cellular network studies without 50/60 Hz line-frequency noise.

For more information, please visit the Digidata 1550B page or download our HumSilencer application notes.

  • Extracellular field potential recordings

    HumSilencer Extracellular field potential recordings

    HumSilencer enables accurate measurement of extracellular field potential recordings of CA1 pyramidal cell body layer of a mouse hippocampus brain slice. Top = HumSilencer-enabled data;bottom = raw data. Data courtesy of John Huguenard, Ph.D., Stanford University

    Whole-cell recordings

    HumSilencer Whole-cell recordings

    HumSilencer enables accurate single measurement in whole-cell recordings from a C. elegans body-wall muscle cell. Top = HumSilencer-enabled data; bottom = raw data. Data courtesy of Zhao-Wen Wang, Ph.D., University of Connecticut Health Center.

  • Macroscopic currents from excised patches

    HumSilencer Macroscopic currents from excised patches

    HumSilencer enables accurate measurement of macroscopic currents from patches excised from an HEK cell at -80V holding and repolarizing potentials. Top = HumSilencer-enabled data; bottom = raw data. Data courtesy of Huanghe Yang, Ph.D., University of California, San Francisco.

    HumSilencer does not distort current-induced action potentials

    HumSilencer does not distort current-induced action potentials

    HumSilencer does not distort current-induced action potentials, which were evoked by injecting a depolarizing current pulse into the recorded muscle cell. Top = HumSilencer-enabled data; middle = raw data; bottom = injected current pulse at 100 nA magnitude and 100 ms duration. Data courtesy of Andrew Voss, Ph.D., Wright State University.

Resources of HumSilencer

Videos and Demos

Axon Digidata 1550B Low Noise Data Acquisition System

Introducing the Axon Digidata 1550B Low Noise Data Acquisition System plus HumSilencer