SpectraMax L Microplate Reader

High sensitivity and high-throughput glow and flash luminescence detection in an upgradeable platform



Pave the pathway to your future with the SpectraMax® L Microplate Luminometer. Measure flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. When you're ready to scale up, the SpectraMax L Reader can give you up to a six-fold increase in throughput with multi-channel configuration upgrade options. SoftMax Pro GxP Software and its software validation package give you comprehensive documentation and extensive data sets in CD-ROM format so you'll be able to meet FDA 21 CFR Part 11 and GxP compliance requirements.


  • Measure to assay lower limits with high sensitivity luminescence detection down to 0.2 fg firefly luciferase (glow) or 20 amol ATP (flash)
  • Read both 96- and 384-well plates with the lowest crosstalk for better signal discrimination with manual or automatic optical aperture switching
  • Capture the full range of an assay by extending the measurable dynamic range to 9 orders of magnitude with simultaneous photon counting and analog detection

Upgradeable Options

  • Higher sample throughput enabled by multiple detector configurations
  • Perform dual luciferase reporter gene assays with dual injector system, capable of reading even very fast flash assays like acridinium esters
  • Measure BRET assays with user-installed filters for separating wavelength peaks
  • Seamless automation coupling with the StakMax Microplate Handling Stacker
  • Upgrade your instrument to meet changing laboratory needs on your schedule by adding detectors or injectors after purchase

A Complete System

  • Monitor and control temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thoroughly mix samples in microplate wells by user-programmable Automix feature with 4 proprietary mixing modes
  • Save on injector maintenance with advanced peristaltic injectors that require less maintenance than traditional syringe injection systems and include an auto-rinse at shutdown

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:

  • SpectraTest ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
  • SoftMax® Pro Software validation package
  • Software tools for FDA 21 CFR Part 11 compliance

The SpectraMax L Microplate Reader supports a number of flash and glow luminescence assays including:

  • Aequorin assays
  • ATP detection
  • Bioluminescence resonance energy transfer (BRET)
  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Enzyme assays
  • Mycoplasma monitoring
  • Reporter gene assays

SoftMax Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of supported application protocols to get you started faster
  • Optional tools for regulated labs with SoftMax Pro GxP to speed compliance
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • The only Mac- and Windows-native application support for seamless exchange of data files between Operating Systems
  • Proven integrations with leading automation and LIMS platforms for partnered solutions

General Specifications

Dimensions (in.) 16.5 W x 17 D x 8.75 H
Dimensions (cm) 42 W x 43 D x 22 H
Weight 36 lbs (16.4 kg)
Power source 230 Vac 50 Hz, 115 Vac 60 Hz
Detector Low-noise photomultiplier tube with simultaneous photon counting and analog mode
Plate format Standard 96- and 384-well microplates, with heights from 13.5-15.5 mm

Performance Specifications

Cross-talk (96-well) 3 x 10-5
Read modes Endpoint, Kinetic, Fast Kinetic, Dual-Read
Injector 2 variable volume injectors (10-100 µl) per read head
Injector accuracy ±1 µL ±2%
Injector precision ±1 µL ±2%
Sensitivity (96-well) < 20 attomol ATP per well, < 0.2 fg firefly luciferase per well
Spectral range 380-630 nm
Dynamic range 9 orders of magnitude
Heater Temperature range: ambient +5°C to 45°C

SpectraMax® L Microplate Reader



SpectraMax L Microplate Reader SpectraMax L
BRET1 Filter Set (480nm/535nm) 0310-5706
BRET2 Filter Set (410nm/515nm) 0310-5707
Barcode reader 0310-5743
SpectraTest LM1 Luminescence Validation Plate 0200-6186
StakMax Handling Stacker Base Unit STAKMAX
Microplate Reader Shelf 9000-0756

Number of Citations*: 259

Latest Citations:
For a complete list, please click here.

Inhibition of GSK3β Stimulates BMP Signaling and Decreases SOST Expression Which Results in Enhanced Osteoblast Differentiation

MAE Schoeman, MJC Moester… - Journal of Cellular …, 2015 - Wiley Online Library
... After 24 hours of treatment with the indicated reagents, luciferase activity was determined
using the Dual-Glo Luciferase assay system (Promega, Fitchburg, WI, USA) with a
SpectraMax L luminometer (Molecular Devices, Sunnyvale, CA, USA). ...

Anti-metastatic immunotherapy based on mucosal administration of flagellin and immunomodulatory P10

FM de Melo, CJM Braga, FV Pereira… - Immunology and cell …, 2015 -
... The reaction was developed with ECL (EMD Millipore), read in Spectramax L equipment
(Molecular Devices) and expressed as Relative Luminescent Units. For immunoglobulin
isotyping, we used the protocol described by Bargieri et al. ...

Hydrogen‐Bonded Multilayers of Tannic Acid as Mediators of T‐Cell Immunity

V KozlovskayaB Xue, W Lei… - Advanced …, 2015 - Wiley Online Library
... of 200 μL. Luminescence was quantified using a SpectraMax L Luminescence
microplate reader and was recorded every 2 min for 1 h after stimulation with 100
ng mL −1 PMA and 1μg mL −1 In. Western Immunoblotting: Whole ...

Characterization of a Bispecific FLT3 X CD3 Antibody in an Improved, Recombinant Format for the Treatment of Leukemia

M Durben, D Schmiedel, M Hofmann, F Vogt… - Molecular …, 2015 -
... 6 hours. Luminescence induced after activation of the Jurkat cells by bispecific
antibodies bound to the NALM-16 cells was measured in a SpectraMax L luminometer
(Molecular Devices, Biberach, Germany). Serum concentrations ...

Glassy‐State Stabilization of a Dominant Negative Inhibitor Anthrax Vaccine Containing Aluminum Hydroxide and Glycopyranoside Lipid A Adjuvants

KJ HassettDJ VanceNK JainN Sahni… - Journal of …, 2015 - Wiley Online Library
... Cell viability was assessed using Cell Titer Glo (Promega, Madison, Wisconsin) and a
Spectramax L Luminometer (Molecular Devices). Neutralizing titers were defined as the
inverse titer that protected at least 50% of the cells from LT. RESULTS. ...

Stenotrophomonas maltophilia bloodstream infection in patients with hematologic malignancies: a retrospective study and in vitro activities of antimicrobial …

SY Cho, DG Lee, SM Choi, C Park… - BMC infectious …, 2015 -
... Relative luminescence units (RLU) were measured using a SpectraMax L luminescence
microplate reader (Molecular Devices, Sunnyvale, CA, USA). The percentage of RLUs compared
to the antibiotic-free controls (%RLU) was calculated, with minimal Page 6. ...

Elastin‐like recombinamers with acquired functionalities for gene‐delivery applications

MJ Piña, SM Alex, FJ Arias, M Santos… - … Research Part A, 2015 - Wiley Online Library
... Promega). The Bright Glo luciferase assay system (Promega) was added in a 1:1
proportion of lysate and luciferase reagent. The light produced was measured using
a luminometer (SpectraMax L, Molecular Devices). The protein ...

Calpain Determines the Propensity of Adult Hippocampal Neural Stem Cells to Autophagic Cell Death Following Insulin Withdrawal

KM Chung, H Park, S Jung, S Ha, SJ Yoo… - STEM …, 2015 - Wiley Online Library
... A freshly prepared dilution of the Calpain-Glo Reagent stock solution was added to the cells and
luminescence was recorded with a plate-reading luminometer (Molecular Devices, Sunnyvale,
CA,; SpectraMax L). The luminescence values ...

[HTML] Corosolic Acid Inhibits Hepatocellular Carcinoma Cell Migration by Targeting the VEGFR2/Src/FAK Pathway

CY Ku, YR Wang, HY Lin, SC Lu, JY Lin - 2015 -
... 40 min. Finally, 50 μL kinase detection reagent was added to introduce luciferase
and samples were measured with a SpectraMax L Microplate reader (Molecular
Device, CA, USA). Rho GTPase activity assay. Huh7 cells were ...

Diurnal rhythms of plasma GLP-1 levels in normal and overweight/obese subjects: lack of effect of weight loss

JSG Muñoz, DJ Rodríguez, JJH Morante - Journal of physiology and …, 2015 - Springer
... levels of biological- ly active forms of GLP-1 (GLP17–36 amide and GLP17– 37) were measured
by fluorescence ELISA through the human glucagon-like peptide 1 (active) kit (Millipore, Billerica,
MA) with a luminescence multi-plate reader (SpectraMax L, Molecular Devices ...

* as of June 26, 2015. Source: Google Scholar.