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NFC-based personalization and enhanced touchscreen workflows provide walk-up usability

The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.

With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.

  • Personalize workflows

    Personalize workflows

    Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.

  • Simplify tasks

    Simplify tasks

    The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.

  • Reduce background noise

    Reduce background noise

    The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.

SpectraMax iD3

Features

  • Connectivity Icon

    QuickSync

    The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.

  • Environmental Control Icon

    Flexible temperature control

    Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.

  • Automation Icon

    Automatic filter identification

    Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.

  • Security Icon

    Enhanced security

    The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.

  • Streamline Icon

    Hybrid optical system

    The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.

  • Flash Assays Icon

    Flash assays captured with ease

    The SpectraMax Injector System with SmartInject® Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.

Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational specifications
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs

 

Applications of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers

  • Absorbance

    Absorbance

    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    Cell Viability

    Cell Viability

    Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.

    Read Application Note 

  • DNA/RNA Quantitation

    DNA/RNA Quantitation

    The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.

    Read Application Note 

    ELISA

    Elisa

    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

  • Fluorescence

    Fluorescence

    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  

    Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

    Learn more  

  • HTRF

    HTRF

    HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
    (This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

    Read Application Note 

    Luminescence

    Luminescence

    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

    Learn more  

  • Reporter Gene Assay

    SpectraMax DuoLuc Reporter Assay High Resolution

    Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.

    Read Application Note 

    TRF, TR-FRET & HTRF

    Time-resolved

    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

    Learn more  

  • Western blotting

    Western Blot Detection

    Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence, and chemiluminescence. We’ll also introduce you to a novel, first-of-its-kind western blot detection system on a multi-mode microplate reader.

    Learn more 

Specifications & Options of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers

 

*Using lowest settings and speed read when available.

Resources of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers

Presentations
Videos & Webinars
SpectraMax iD3 Multi-Mode Microplate Reader

Overview of SpectraMax iD3 Multi-Mode Microplate Reader

SpectraMax iD3 Tutorial Video - Getting to know your new SpectraMax iD3

Getting to know your new SpectraMax iD3

SpectraMax iD5 Multi-Mode Microplate Reader

New SpectraMax iD5 Multi-Mode Microplate Reader

SpectraMax iD5 Microplate Reader

SpectraMax iD5 Multi-Mode Microplate Reader

Antigen / Immunogen Discovery and Optimization

Immunology and Vaccine Development Workflow

Hybridoma Workflow

Hybridoma Workflow

Week 5 Video Thumbnail

Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?

Week 4 Video Thumbnail

Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy

Week 3 Video Thumbnail

Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"

 Week 1 Video Thumbnail

Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!

Week 2 Video thumbnail

Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!

Microplate Readers from ELISA to AlphaScreen

Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen

Microplate based Detection

Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening

ELISA endpoint protocol

How to configure an ELISA endpoint protocol

SpectraMax iD3

SpectraMax iD5

SpectraMax iD3 Tutorial Video - Viewing Results

Viewing Results on SpectraMax iD3 Multi-Mode Microplate Reader

SpectraMax iD3 Tutorial Video - Starting a Plate Read

Starting a Plate Read using SpectraMax iD3 Multi Mode

SpectraMax iD3 Tutorial Video - Personalizing your SpectraMax iD3

Personalizing Your SpectraMax iD3

SpectraMax iD5 Tutorial Video - Viewing results on the SpectraMax iD5

Viewing Results on the SpectraMax iD5

SpectraMax iD5 Tutorial Video - Starting a plate read on the Spectramax iD5

Starting a Plate Read on the Spectramax iD5

SpectraMax iD5 Tutorial Video - Personalizing your SpectraMax iD5

Personalizing Your SpectraMax iD5

  • Citation
    Dated: Apr 09, 2021
    Publication Name: Journal of Neuro-Oncology

    Dura promotes metastatic potential in prostate cancer through the CXCR2 pathway

    Spinal metastases are common in cancer. This preferential migration/growth in the spine is not fully understood. Dura has been shown to affect the surrounding microenvironment and promote cancer growth. Here, we investigate the role of dural cytokines in promoting the metastatic potential of prostate cancer (PCa) and the involvement of the CXCR2… View more

    Spinal metastases are common in cancer. This preferential migration/growth in the spine is not fully understood. Dura has been shown to affect the surrounding microenvironment and promote cancer growth. Here, we investigate the role of dural cytokines in promoting the metastatic potential of prostate cancer (PCa) and the involvement of the CXCR2 signaling pathway.

    Contributors: Michael J. Strong, Sabrina Rocco, Russell Taichman, Gregory A. Clines & Nicholas J. Szerlip  
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  • Citation
    Dated: Dec 01, 2020
    Publication Name: Science Direct

    Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts

    Human gingival fibroblasts (HGF) play a vital role in wound healing, oral cancer, and are among the first cells being exposed to e-cigarette vapor (eCV) or cigarette smoke (CS) during inhalation. Although the cell-damaging effect of CS has been well studied, the effects of eCV on gingival cells are still unclear. The aim of this in vitro study was… View more

    Human gingival fibroblasts (HGF) play a vital role in wound healing, oral cancer, and are among the first cells being exposed to e-cigarette vapor (eCV) or cigarette smoke (CS) during inhalation. Although the cell-damaging effect of CS has been well studied, the effects of eCV on gingival cells are still unclear. The aim of this in vitro study was to compare the effects of eCV and CS on HGF in terms of proliferation, metabolic activity, cell death, and formation of reactive oxygen species (ROS).

    Contributors: M.F.Vermehrena1N.Wiesmannab1J.DeschnercJ.BriegerbB.Al-NawasaP.W.Kämmerera  
    Go to article

  • Citation
    Dated: Oct 01, 2020
    Publication Name: AHA Journals

    HIV Antivirals Affect Endothelial Activation and Endothelial-Platelet Crosstalk

    People living with human immunodeficiency virus (PLHIV) on effective antiretroviral therapy are at increased risk of cardiovascular complications, possibly due to off-target drug effects. Some studies have associated antiretroviral therapy with increased risk of myocardial infarction and endothelial dysfunction, but a link between endothelial… View more

    People living with human immunodeficiency virus (PLHIV) on effective antiretroviral therapy are at increased risk of cardiovascular complications, possibly due to off-target drug effects. Some studies have associated antiretroviral therapy with increased risk of myocardial infarction and endothelial dysfunction, but a link between endothelial function and antiretrovirals has not been established.

    Contributors: Akif A Khawaja, Kirk A Taylor, Andrew O Lovell, Mark Nelson, Brian Gazzard, Marta Boffito, and Michael Emerson  
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  • Citation
    Dated: Sep 01, 2020
    Publication Name: Journal of Perinatology

    Difference in levels of SARS-CoV-2 S1 and S2 subunits- and nucleocapsid protein-reactive SIgM/IgM, IgG and SIgA/IgA antibodies in human milk

    SARS-CoV-2 is the novel coronavirus discovered in 2019 (2019-nCoV) that is responsible for the severe acute respiratory syndrome (SARS) pandemic. The coronavirus disease 2019 (COVID-19) was first observed in China. SARS-CoV-2 is responsible for pandemic-level infections globally, including the United States [1]. To induce COVID-19, the spike 1 (S1… View more

    SARS-CoV-2 is the novel coronavirus discovered in 2019 (2019-nCoV) that is responsible for the severe acute respiratory syndrome (SARS) pandemic. The coronavirus disease 2019 (COVID-19) was first observed in China. SARS-CoV-2 is responsible for pandemic-level infections globally, including the United States [1]. To induce COVID-19, the spike 1 (S1) subunit attaches to angiotensin-converting enzyme-2 (ACE-2) receptors on the cellular surface of the human respiratory tract [2].

    Contributors: Veronique Demers-Mathieu, Dung M. Do, Gabrielle B. Mathijssen, David A. Sela, Antti Seppo, Kirsi M. Järvinen & Elena Medo  
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  • Citation
    Dated: Apr 01, 2020
    Publication Name: Ecotoxicology and Environmental Safety

    Inhibition of pancreatic lipase by environmental xenoestrogens

    Environmental xenoestrogens are the most accessible endocrine disrupting chemicals that have been reported with harmful effects on human health. Although the influences of xenoestrogens on the endocrine system have been extensively studied, it remains unclear whether these xenoestrogens can affect the digestive system in mammals. This study aimed… View more

    Environmental xenoestrogens are the most accessible endocrine disrupting chemicals that have been reported with harmful effects on human health. Although the influences of xenoestrogens on the endocrine system have been extensively studied, it remains unclear whether these xenoestrogens can affect the digestive system in mammals. This study aimed to investigate the inhibitory effects and the underlying mechanism of six non-steroidal synthetic estrogens (including hexestrol, diethylstilbestrol, dienestrol, bisphenol A, bisphenol AF and bisphenol Z) on pancreatic lipase (PL), a key digestive enzyme responsible for lipid digestion and absorption in mammals. The results clearly demonstrated that hexestrol, diethylstilbestrol and dienestrol exhibited strong inhibition on PL, with the IC50 values of less than 1.0 μM. Further investigations elucidated that these three synthetic estrogens functioned as mixed inhibitors of PL, with the Ki values of less than 1 μM. Moreover, molecular dynamics simulations showed that diethylstilbestrol and its analogues might block the binding of substrate on PL via occupying the portal to the active site of PL and thereby inhibit the hydrolytic activity of this key enzyme. Collectively, these results suggested that diethylstilbestrol and its analogues were potent PL inhibitors, which might play a profound role in lipid absorption and weight gain in mammals.

    Contributors: Qing Hu, Xiao-Qing Guan, Li-LinSong, Hao-Nan Wang, Yuan Xiong, Jun-Ling Liu, Heng Yin, Yun-Feng Cao, Jie Hou, Ling Yang, Guang-Bo Ge  
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  • Citation
    Dated: Jan 01, 2020
    Publication Name: Chemosphere

    Characterization of boscalid-induced oxidative stress and neurodevelopmental toxicity in zebrafish embryos

    Boscalid is a widely used fungicide in agriculture and has been frequently detected in both environments and agricultural products. However, evidence on the neurotoxic effect of boscalid is scarce. In this study, zebrafish served as an animal model to investigate the toxic effects and mechanisms of boscalid on aquatic vertebrates or higher animals… View more

    Boscalid is a widely used fungicide in agriculture and has been frequently detected in both environments and agricultural products. However, evidence on the neurotoxic effect of boscalid is scarce. In this study, zebrafish served as an animal model to investigate the toxic effects and mechanisms of boscalid on aquatic vertebrates or higher animals. And we unravelled that boscalid induced developmental defects associated with oxidative stress. Developmental defects, including head deformity, hypopigmentation, decreased number of newborn neurons, structural defects around the ventricle, enlarged intercellular space in the brain, and nuclear concentration, were observed in zebrafish embryos after boscalid exposure at 48 hpf. Interestingly, we found that boscalid might directly induce oxidative stress and alter the activity of ATPase, which in turn disrupted the expression of genes involved in neurodevelopment and transmitter-transmitting signalings and melanocyte differentiation and melanin synthesis signalings. Ultimately, the differentiation of nerve cells and melanocytes were both impacted and the synthesis of melanin was inhibited, leading to morphological abnormalities. Additionally, exposure to boscalid led to less and imbalance motion and altered tendency of locomotor in larval fish. Collectively, our results provide new evidences for a comprehensive assessment of its toxicity and a warning for its residues in environment and agricultural products.

    Contributors: Honglei Wanga, Zhen Meng, FashengLiu, Liqun Zhou, MeileSu, Yunlong Meng, Shouhua Zhang, Xinjun Liao, Zigang Cao, Huiqiang Lu  
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