SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Multi-user microplate readers with a large touchscreen and NFC functionality
NFC-based personalization and enhanced touchscreen workflows provide walk-up usability
The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.
Features
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QuickSync
The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.
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Flexible temperature control
Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.
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Automatic filter identification
Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.
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Enhanced security
The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.
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Hybrid optical system
The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.
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Flash assays captured with ease
The SpectraMax Injector System with SmartInject® Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.
Proven GxP solutions to assure data integrity and compliance
Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.
- Best-in-class microplate readers and washers support all your assay needs
- IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
- Software installation services verify and document that required components are installed to operational specifications
- Software validation service supports FDA 21 CFR Part 11 guidelines
- Validation plates test the performance of your microplate reader using traceable materials for reliable results
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Blog
FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs
FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs
The regulations for food and drug in the United States, described in the Title 21 of the Code of Federal Regulations, are critical in ensuring safe and ethical drug administration.…
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Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Applications of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
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Absorbance
Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.
Absorbance
Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.
Cell Health
Cell viability refers to the number of healthy cells in a population and can be evaluated using assays that measure enzyme activity, cell membrane integrity, ATP production, and other indicators. These methods can employ luminescent, fluorescent, or colorimetric readouts as indicators of general cell viability or even specific cellular pathways. Cytotoxicity and cell viability assays are often used to assess a drug or other treatment’s effect, and are valuable tools in the search for new therapeutics, as well as advancing our understanding of how normal cells function.
Cell Health
Cell viability refers to the number of healthy cells in a population and can be evaluated using assays that measure enzyme activity, cell membrane integrity, ATP production, and other indicators. These methods can employ luminescent, fluorescent, or colorimetric readouts as indicators of general cell viability or even specific cellular pathways. Cytotoxicity and cell viability assays are often used to assess a drug or other treatment’s effect, and are valuable tools in the search for new therapeutics, as well as advancing our understanding of how normal cells function.
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Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
Cellular Signaling
Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.
Cellular Signaling
Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.
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DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.
DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
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Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
Fluorescence Polarization (FP)
Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.
Fluorescence Polarization (FP)
Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.
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HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)Luminescence
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.
Luminescence
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.
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Microbiology and Contaminant
Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s biomass, and microbes in the human body outnumber human cells by 10 to 1. These microorganisms provide great benefit to us and are also vital to many fields of research from medicine to alternative energy production. On the other hand, monitoring for microbes and the toxic substances they produce is necessary to ensure the safety of pharmaceutical products. Scientists whose research relies on mammalian cells must carefully monitor these cultures for unwanted microbial contaminants to ensure that their experimental results are reliable.
Microbiology and Contaminant
Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s biomass, and microbes in the human body outnumber human cells by 10 to 1. These microorganisms provide great benefit to us and are also vital to many fields of research from medicine to alternative energy production. On the other hand, monitoring for microbes and the toxic substances they produce is necessary to ensure the safety of pharmaceutical products. Scientists whose research relies on mammalian cells must carefully monitor these cultures for unwanted microbial contaminants to ensure that their experimental results are reliable.
Nucleic Acid (DNA/RNA) Quantitation and Analysis
Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA) consists of a double strand of pairs of nucleotides, while ribonucleic acid (RNA) is typically a single strand. In DNA, the nucleotides are adenine, cytosine, guanine, and thymine, while RNA contains uracil instead of thymine. DNA makes up the genetic material of all organisms, encoding the information cells need to synthesize proteins.
Nucleic Acid (DNA/RNA) Quantitation and Analysis
Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA) consists of a double strand of pairs of nucleotides, while ribonucleic acid (RNA) is typically a single strand. In DNA, the nucleotides are adenine, cytosine, guanine, and thymine, while RNA contains uracil instead of thymine. DNA makes up the genetic material of all organisms, encoding the information cells need to synthesize proteins.
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Protein Detection, Quantitation and Analysis
Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.
Protein Detection, Quantitation and Analysis
Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
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TRF, TR-FRET & HTRF
Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.
TRF, TR-FRET & HTRF
Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.
Western blotting
Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence, and chemiluminescence. We’ll also introduce you to a novel, first-of-its-kind western blot detection system on a multi-mode microplate reader.
Western blotting
Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence, and chemiluminescence. We’ll also introduce you to a novel, first-of-its-kind western blot detection system on a multi-mode microplate reader.
Specifications & Options of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Resources of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
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Blog
FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs
FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs
The regulations for food and drug in the United States, described in the Title 21 of the Code of Federal Regulations, are critical in ensuring safe and ethical drug administration.…
-
Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Application Note
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about…
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Application Note
Optimized HTRF settings for SpectraMax multi-mode microplate readers
Optimized HTRF settings for SpectraMax multi-mode microplate readers
HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers.
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Application Note
Optimized settings for Transcreener FI Assays on SpectraMax iD3 and iD5 microplate readers
Optimized settings for Transcreener FI Assays on SpectraMax iD3 and iD5 microplate readers
Transcreener® HTS is a universal, highthroughput, biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes. Many of these…
Flyer
Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay
Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay
ATP standards ranging from 1 μM to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). 50 μL of standard was pipetted into the wells of a white…
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Application Note
Detect GPCR activity with the cAMP-Gs HiRange HTRF assay
Detect GPCR activity with the cAMP-Gs HiRange HTRF assay
HTRF® is a versatile technology developed by Cisbio for detecting biomolecular interactions.
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Application Note
Measure cancer cell viability using a homogeneous, stable luminescence assay
Measure cancer cell viability using a homogeneous, stable luminescence assay
Luminescent cell viability assays offer sensitivity and an easy workflow for monitoring the effects of various experimental conditions.
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Application Note
HTRF profiling services on SpectraMax iD5 Multi-Mode Microplate Reader
HTRF profiling services on SpectraMax iD5 Multi-Mode Microplate Reader
Cisbio’s Custom Solutions Laboratory team has worked in collaboration with Molecular Devices to generate relevant data promoting the expert profiling service activity completed by…
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Application Note
Measure p53-MDM2 protein interaction with NanoBRET technology
Measure p53-MDM2 protein interaction with NanoBRET technology
We describe validation of the SpectraMax® iD5 reader with the NanoBRET™ PPI Control Pair, consisting of the interacting protein partners p53 and MDM2.
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Application Note
Streamline BCA-based protein quantitation on the SpectraMax iD5 reader
Streamline BCA-based protein quantitation on the SpectraMax iD5 reader
Learn how to quantitate a cellular protein sample with BCA protein assay kits on the SpectraMax® iD5 Multi-Mode Microplate Reader.
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Application Note
Faster ELISA results with CatchPoint SimpleStep ELISA kits
Faster ELISA results with CatchPoint SimpleStep ELISA kits
CatchPoint SimpleStep ELISA kits give users a 90-minute ELISA workflow, a nearly two-thirds reduction in time compared to conventional ELISAs.
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Application Note
Measure intracellular reactive oxygen species using far-red fluorescence
Measure intracellular reactive oxygen species using far-red fluorescence
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen resulting from aerobic cellular metabolism. These chemical species are involved in cellular processes…
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Application Note
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most…
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Application Note
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
In this application note we show how the SpectraMax® Paradigm® Multi-Mode Microplate Reader is used to perform robust, no-wash cytokine assays with excellent Z’ factors. HTRF® is a…
Brochure
SpectraMax iD5 Multi-Mode Microplate Reader
SpectraMax iD5 Multi-Mode Microplate Reader
Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to h…
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Brochure
SpectraMax iD3 Multi-Mode Microplate Reader
SpectraMax iD3 Multi-Mode Microplate Reader
The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With optimized…
eBook
The Ultimate Guide to Microplate Reader Solutions
The Ultimate Guide to Microplate Reader Solutions
Evaluating microplate readers doesn’t have to be overwhelming. First, consider your application needs. If your budget is modest, a single-mode reader dedicated to your main applicati…
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Application Note
Assess virus neutralization with a rapid, HTS-friendly assay
Assess virus neutralization with a rapid, HTS-friendly assay
The worldwide COVID-19 pandemic caused by the SARS-CoV-2 virus has necessitated the fast-tracked development of many research tools for understanding this virus’s pathogenesis, as well…
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Application Note
Cell-based measurement of ERK1/2 phosphorylation with THUNDER TR-FRET assay
Cell-based measurement of ERK1/2 phosphorylation with THUNDER TR-FRET assay
The MAPK/ERK cell signaling pathway begins with the activation of a receptor tyrosine kinase located at the cell surface, involves a series of kinase phosphorylation steps (kinase…
Publications
Project Profile: Organoid Innovation Center
Project Profile: Organoid Innovation Center
Lab Manager speaks to Dan O’Connor, vice president, drug discovery, Molecular Devices, about the company’s Organoid Innovation Center in San Jose, CA. The center is 180 sq. ft., with…
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Publications
Shaping the Future of Organoid Research
Shaping the Future of Organoid Research
Molecular Devices, a provider of innovative life science technology, recently unveiled a brand new, first-of-its-kind Organoid Innovation Center. Situated at the company’s global hea…
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Application Note
Detect SARS-CoV-2 IgG in serum samples with a luminescent immunoassay
Detect SARS-CoV-2 IgG in serum samples with a luminescent immunoassay
As part of the immune response to SARS-CoV-2, infected individuals produce virus-specific antibodies that are detectable in the blood within one to three weeks after the onset of…
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Application Note
Measure viral-induced cytopathic effects with a quantitative luminescence assay
Measure viral-induced cytopathic effects with a quantitative luminescence assay
Viral infection of mammalian cells often reduces cell viability and causes visible effects upon the cells such as changes in shape or size, or fusion with adjacent cells. These changes…
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Application Note
High-throughput IgG quantitation platform for clone screening during drug discovery and development
High-throughput IgG quantitation platform for clone screening during drug discovery and development
Biologic drugs are the largest and fastest growing segment of the pharmaceutical industry with sales of €500bn and an annual growth of 8%. Every manufacturing process for potential…
Customer Breakthrough
National Horizons Centre uses the SpectraMax iD5 & SoftMax Pro Software to train the next generation of scientists
National Horizons Centre uses the SpectraMax iD5 & SoftMax Pro Software to train the next generation of scientists
A UK centre of excellence for the bioscience industry
Opened in Spring 2019, the National Horizons Centre (NHC) is an exciting £22M state-of-the art, purpose-…
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eBook
Accelerate vaccine development
Accelerate vaccine development
Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
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Blog
The history of ELISA from creation to COVID-19 research
The history of ELISA from creation to COVID-19 research
Over the past three decades, ELISA or enzyme-linked immunosorbent assay, has become vital to many areas of research and has shown to have many applications—from detecting food and…
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Application Note
Determine Kd for the glucagon GLP-1 receptor with Tag-lite HTRF technology
Determine Kd for the glucagon GLP-1 receptor with Tag-lite HTRF technology
The Tag-lite HTRF platform from Cisbio enables efficient labeling of proteins of interest on targeted sites with HTRF fluorophores. A cell surface receptor can be inserted into a SNAP-…
Infographic
History of ELISA
History of ELISA
ELISA (enzyme-linked immunosorbent assay) is a method used to quantitatively detect an antigen within a sample. An antigen is a toxin or other foreign substance, for example a flu vi…
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Customer Breakthrough
Université de Paris use the SpectraMax iD3 to advance understanding of Extracellular Vesicles
Université de Paris use the SpectraMax iD3 to advance understanding of Extracellular Vesicles
The main goal of Dr. Grégory Lavieu’s group at the Université de Paris is to understand at both cellular and molecular levels how Extracellular Vesicles (EVs), also called exosomes,…
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Application Note
Development of optimized workflows to enable calcium mobilization assays on the SpectraMax iD3 and iD5 readers
Development of optimized workflows to enable calcium mobilization assays on the SpectraMax iD3 and iD5 readers
As a laboratory’s requirements often change according to ongoing research needs, having a multi-mode microplate reader that offers multiple detection modes such as absorbance,…
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Customer Breakthrough
RiboPro use the SpectraMax iD3 to advance research into life-changing mRNA therapeutics
RiboPro use the SpectraMax iD3 to advance research into life-changing mRNA therapeutics
RiboPro is a spin-off of Dutch biotechnology company Mercurna. Based in The Netherlands, the RiboPro team design and produce high-quality messenger RNA (mRNA) for academic and indust…
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Application Note
HTRF IP-One Gq assay on SpectraMax Readers
HTRF IP-One Gq assay on SpectraMax Readers
HTRF® is a versatile technology developed by Cisbio Bioassays for detecting biomolecular interactions.
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Application Note
Establishing and optimizing a fluorescence polarization assay
Establishing and optimizing a fluorescence polarization assay
This technical note is designed to provide information to help the user define the optimal experimental conditions for converting existing assays to a robust fluorescence polarization…
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Brochure
SpectraMax i Series Multi-Mode Microplate Readers
SpectraMax i Series Multi-Mode Microplate Readers
The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Whether you are looking for an intuitive, easy-to-use reader for y…
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eBook
From ELISA to epigenetics with HTRF
From ELISA to epigenetics with HTRF
HTRF® is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Assays encompass the areas of G protein-coupled receptors (GPCR), kinases and cel…
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Application Note
Better metrics for comparing instruments and assays
Better metrics for comparing instruments and assays
When comparing instruments, one must choose a metric by which to measure their performance. Typically, one is interested in how “sensitive” an instrument is, but sensitivity can be…
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Application Note
Comparing performance of SpectraMax absorbance microplate readers using an endotoxin assay
Comparing performance of SpectraMax absorbance microplate readers using an endotoxin assay
Control Standard Endotoxin (CSE) was reconstituted with the volume of LAL reagent water (LRW) indicated on the Certificate of Analysis included with the kit to yield a solution of 100…
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Application Note
Optimized settings for Transcreener TR-FRET assays on SpectraMax iD5 and i3x readers
Optimized settings for Transcreener TR-FRET assays on SpectraMax iD5 and i3x readers
Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which…
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Customer Breakthrough
The University of Rouen in Normandy uses the SpectraMax iD3 and FlexStation 3 for calcium studies, and in its teaching laboratories
The University of Rouen in Normandy uses the SpectraMax iD3 and FlexStation 3 for calcium studies, and in its teaching laboratories
Studies carried out in the “Peptide Synthesis and Functional Screening” department of the Cell Imaging Platform of the University of Rouen in Haute-Normandie (…
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Application Note
Determine total aflatoxin in cannabis using an ELISA method
Determine total aflatoxin in cannabis using an ELISA method
As cannabis is legalized in more states across the US, state agencies are setting their own regulations and requirements for growers, retailers, and testing laboratories. For example,…
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Application Note
Evaluate anti-inflammatory compounds using a multiparametric THP-1 cell-based assay
Evaluate anti-inflammatory compounds using a multiparametric THP-1 cell-based assay
Macrophages originate from blood monocytes that leave the circulation and penetrate various tissues where they differentiate into macrophages.
Scientific Poster
Multi-parametric phenotypic THP-1 cell differentiation and cytokine secretion assay for evaluation of anti-inflammatory compounds
Multi-parametric phenotypic THP-1 cell differentiation and cytokine secretion assay for evaluation of anti-inflammatory compounds
Macrophages originate from blood monocytes that leave the circulation to differentiate into various tissues. Macrophages are involved in the detection and phagocytosis of bacteria an…
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eBook
Explore cells from the inside out
Explore cells from the inside out
Study protein-protein interaction to cell cycle analysis in spheroids with patented microplate reader technologies.…
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Application Note
Measure long-term cell growth using a discontinuous kinetic reading
Measure long-term cell growth using a discontinuous kinetic reading
Many biological experiments require monitoring cell growth or measuring enzymatic changes over long periods of time (hours, days or even weeks). In addition, certain model organisms…
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Application Note
Measure intrinsic tryptophan fluorescence on the SpectraMax iD3 microplate reader
Measure intrinsic tryptophan fluorescence on the SpectraMax iD3 microplate reader
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Tryptophan, which excites maximally around 270-280 nm and has an…
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Application Note
Measuring cell health on the SpectraMax iD3 reader with cell viability assays
Measuring cell health on the SpectraMax iD3 reader with cell viability assays
Measuring cell health from the multiple vantage points of viability and apoptotic pathways can provide insight into the impacts of a variety of experimental treatments including drug…
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Application Note
Streamline the workflow for measuring IgG in cell line development
Streamline the workflow for measuring IgG in cell line development
The process of cell line development for the production of antibody-based therapeutics can vary widely amongst researchers. The ultimate goal is to find a clone that stably produces…
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Application Note
DNA and RNA absorbance measurements using SpectraMax Microplate Readers
DNA and RNA absorbance measurements using SpectraMax Microplate Readers
Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Since then, microplate measurements of DNA, RNA,…
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Application Note
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite outgrowth and is regulated by…
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Application Note
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA Boosters
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA Boosters
Traditional ELISA (enzyme-linked immunosorbent assay) methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance…
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Application Note
Connectivity and user interaction with the SpectraMax iD3 Multi-Mode Microplate Reader
Connectivity and user interaction with the SpectraMax iD3 Multi-Mode Microplate Reader
The 21st century laboratory has become a fast-paced environment characterized by frequent changes in assay technology and increased employee turnover, leaving less time for complex…
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Application Note
Detection and quantitation of protein with ScanLater Western Blot Detection System
Detection and quantitation of protein with ScanLater Western Blot Detection System
Protein detection is important for pharmaceutical and clinical research today, and western blots are among the most common methods employed for this purpose. Various techniques are…
Overview of SpectraMax iD3 Multi-Mode Microplate Reader
Getting to know your new SpectraMax iD3
New SpectraMax iD5 Multi-Mode Microplate Reader
SpectraMax iD5 Multi-Mode Microplate Reader
Critical Considerations for Choosing the Right Microplate Reader
Immunology and Vaccine Development Workflow
Hybridoma Workflow
Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?
Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy
Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"
Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!
Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!
Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen
Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening
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Number of Citations*: 766
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Dated: Apr 09, 2021Publication Name: Journal of Neuro-OncologyDura promotes metastatic potential in prostate cancer through the CXCR2 pathway
Spinal metastases are common in cancer. This preferential migration/growth in the spine is not fully understood. Dura has been shown to affect the surrounding microenvironment and promote cancer growth. Here, we investigate the role of dural cytokines in promoting the metastatic potential of prostate cancer (PCa) and the involvement of the CXCR2… View moreSpinal metastases are common in cancer. This preferential migration/growth in the spine is not fully understood. Dura has been shown to affect the surrounding microenvironment and promote cancer growth. Here, we investigate the role of dural cytokines in promoting the metastatic potential of prostate cancer (PCa) and the involvement of the CXCR2 signaling pathway.
Contributors: Michael J. Strong, Sabrina Rocco, Russell Taichman, Gregory A. Clines & Nicholas J. Szerlip
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Dated: Dec 01, 2020Publication Name: Science Direct
Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts
Human gingival fibroblasts (HGF) play a vital role in wound healing, oral cancer, and are among the first cells being exposed to e-cigarette vapor (eCV) or cigarette smoke (CS) during inhalation. Although the cell-damaging effect of CS has been well studied, the effects of eCV on gingival cells are still unclear. The aim of this in vitro study was… View moreHuman gingival fibroblasts (HGF) play a vital role in wound healing, oral cancer, and are among the first cells being exposed to e-cigarette vapor (eCV) or cigarette smoke (CS) during inhalation. Although the cell-damaging effect of CS has been well studied, the effects of eCV on gingival cells are still unclear. The aim of this in vitro study was to compare the effects of eCV and CS on HGF in terms of proliferation, metabolic activity, cell death, and formation of reactive oxygen species (ROS).
Contributors: M.F.Vermehrena1N.Wiesmannab1J.DeschnercJ.BriegerbB.Al-NawasaP.W.Kämmerera
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Dated: Oct 01, 2020Publication Name: AHA Journals
HIV Antivirals Affect Endothelial Activation and Endothelial-Platelet Crosstalk
People living with human immunodeficiency virus (PLHIV) on effective antiretroviral therapy are at increased risk of cardiovascular complications, possibly due to off-target drug effects. Some studies have associated antiretroviral therapy with increased risk of myocardial infarction and endothelial dysfunction, but a link between endothelial… View morePeople living with human immunodeficiency virus (PLHIV) on effective antiretroviral therapy are at increased risk of cardiovascular complications, possibly due to off-target drug effects. Some studies have associated antiretroviral therapy with increased risk of myocardial infarction and endothelial dysfunction, but a link between endothelial function and antiretrovirals has not been established.
Contributors: Akif A Khawaja, Kirk A Taylor, Andrew O Lovell, Mark Nelson, Brian Gazzard, Marta Boffito, and Michael Emerson
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Dated: Sep 01, 2020Publication Name: Journal of Perinatology
Difference in levels of SARS-CoV-2 S1 and S2 subunits- and nucleocapsid protein-reactive SIgM/IgM, IgG and SIgA/IgA antibodies in human milk
SARS-CoV-2 is the novel coronavirus discovered in 2019 (2019-nCoV) that is responsible for the severe acute respiratory syndrome (SARS) pandemic. The coronavirus disease 2019 (COVID-19) was first observed in China. SARS-CoV-2 is responsible for pandemic-level infections globally, including the United States [1]. To induce COVID-19, the spike 1 (S1… View moreSARS-CoV-2 is the novel coronavirus discovered in 2019 (2019-nCoV) that is responsible for the severe acute respiratory syndrome (SARS) pandemic. The coronavirus disease 2019 (COVID-19) was first observed in China. SARS-CoV-2 is responsible for pandemic-level infections globally, including the United States [1]. To induce COVID-19, the spike 1 (S1) subunit attaches to angiotensin-converting enzyme-2 (ACE-2) receptors on the cellular surface of the human respiratory tract [2].
Contributors: Veronique Demers-Mathieu, Dung M. Do, Gabrielle B. Mathijssen, David A. Sela, Antti Seppo, Kirsi M. Järvinen & Elena Medo
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Dated: Apr 01, 2020Publication Name: Ecotoxicology and Environmental Safety
Inhibition of pancreatic lipase by environmental xenoestrogens
Environmental xenoestrogens are the most accessible endocrine disrupting chemicals that have been reported with harmful effects on human health. Although the influences of xenoestrogens on the endocrine system have been extensively studied, it remains unclear whether these xenoestrogens can affect the digestive system in mammals. This study aimed… View moreEnvironmental xenoestrogens are the most accessible endocrine disrupting chemicals that have been reported with harmful effects on human health. Although the influences of xenoestrogens on the endocrine system have been extensively studied, it remains unclear whether these xenoestrogens can affect the digestive system in mammals. This study aimed to investigate the inhibitory effects and the underlying mechanism of six non-steroidal synthetic estrogens (including hexestrol, diethylstilbestrol, dienestrol, bisphenol A, bisphenol AF and bisphenol Z) on pancreatic lipase (PL), a key digestive enzyme responsible for lipid digestion and absorption in mammals. The results clearly demonstrated that hexestrol, diethylstilbestrol and dienestrol exhibited strong inhibition on PL, with the IC50 values of less than 1.0 μM. Further investigations elucidated that these three synthetic estrogens functioned as mixed inhibitors of PL, with the Ki values of less than 1 μM. Moreover, molecular dynamics simulations showed that diethylstilbestrol and its analogues might block the binding of substrate on PL via occupying the portal to the active site of PL and thereby inhibit the hydrolytic activity of this key enzyme. Collectively, these results suggested that diethylstilbestrol and its analogues were potent PL inhibitors, which might play a profound role in lipid absorption and weight gain in mammals.
Contributors: Qing Hu, Xiao-Qing Guan, Li-LinSong, Hao-Nan Wang, Yuan Xiong, Jun-Ling Liu, Heng Yin, Yun-Feng Cao, Jie Hou, Ling Yang, Guang-Bo Ge
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Dated: Jan 01, 2020Publication Name: Chemosphere
Characterization of boscalid-induced oxidative stress and neurodevelopmental toxicity in zebrafish embryos
Boscalid is a widely used fungicide in agriculture and has been frequently detected in both environments and agricultural products. However, evidence on the neurotoxic effect of boscalid is scarce. In this study, zebrafish served as an animal model to investigate the toxic effects and mechanisms of boscalid on aquatic vertebrates or higher animals… View moreBoscalid is a widely used fungicide in agriculture and has been frequently detected in both environments and agricultural products. However, evidence on the neurotoxic effect of boscalid is scarce. In this study, zebrafish served as an animal model to investigate the toxic effects and mechanisms of boscalid on aquatic vertebrates or higher animals. And we unravelled that boscalid induced developmental defects associated with oxidative stress. Developmental defects, including head deformity, hypopigmentation, decreased number of newborn neurons, structural defects around the ventricle, enlarged intercellular space in the brain, and nuclear concentration, were observed in zebrafish embryos after boscalid exposure at 48 hpf. Interestingly, we found that boscalid might directly induce oxidative stress and alter the activity of ATPase, which in turn disrupted the expression of genes involved in neurodevelopment and transmitter-transmitting signalings and melanocyte differentiation and melanin synthesis signalings. Ultimately, the differentiation of nerve cells and melanocytes were both impacted and the synthesis of melanin was inhibited, leading to morphological abnormalities. Additionally, exposure to boscalid led to less and imbalance motion and altered tendency of locomotor in larval fish. Collectively, our results provide new evidences for a comprehensive assessment of its toxicity and a warning for its residues in environment and agricultural products.
Contributors: Honglei Wanga, Zhen Meng, FashengLiu, Liqun Zhou, MeileSu, Yunlong Meng, Shouhua Zhang, Xinjun Liao, Zigang Cao, Huiqiang Lu
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