SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Multi-user microplate readers with a large touchscreen and NFC functionality
NFC-based personalization and enhanced touchscreen workflows provide walk-up usability
The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.
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Personalize workflows
Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.
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Simplify tasks
The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.
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Reduce background noise
The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.
SpectraMax iD5 Multi-Mode Microplate Reader
Features
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QuickSync
The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.
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Flexible temperature control
Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.
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Automatic filter identification
Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.
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Enhanced security
The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.
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Application Note
Measure intracellular reactive oxygen species using far-red…
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen resulting from aerobic cellular metabolism. These chemical species…
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Application Note
Monitor NF-κB activation with a sensitive dual reporter assay on…
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease…
Applications of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
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Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
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DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.
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Western Blot Detection
Western blotting is among the most common methods employed for the detection and quantitation of specific proteins. In this method, protein sample, e.g. from a cell lysate, is first separated by size on an SDS-PAGE gel. Proteins are then transferred to a nitrocellulose or PVDF membrane, which is probed with antibody specific to the protein of interest. Various techniques are used to detect proteins on western blot membranes including fluorescence and chemiluminescence.
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HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.) -
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Specifications & Options of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Resources of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
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Application Note
Measure intracellular reactive oxygen species using far-red…
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen resulting from aerobic cellular metabolism. These chemical species…
-
Application Note
Monitor NF-κB activation with a sensitive dual reporter assay on the…
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease…
-
Application Note
Measure intrinsic tryptophan fluorescence on the SpectraMax iD3…
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Tryptophan, which excites…
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Application Note
Measuring cell health on the SpectraMax iD3 reader with cell…
Measuring cell health from the multiple vantage points of viability and apoptotic pathways can provide insight into the impacts of a variety of…
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Application Note
Streamline the workflow for measuring IgG in cell line development
The process of cell line development for the production of antibody-based therapeutics can vary widely amongst researchers. The ultimate goal is to…
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Application Note
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA…
Traditional ELISA (enzyme-linked immunosorbent assay) methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB)…
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Application Note
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and…
Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite…
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Application Note
Connectivity and user interaction with the SpectraMax iD3 Multi-Mode…
The 21st century laboratory has become a fast-paced environment characterized by frequent changes in assay technology and increased employee turnover…
Data Sheet
SpectraMax iD3 Multi-Mode Microplate Reader
The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your res…
Product Brochure
SpectraMax iD5 Multi-Mode Microplate Reader
Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is th…
Videos
Overview of SpectraMax iD3 Multi-Mode Microplate Reader
Getting to know your new SpectraMax iD3
New SpectraMax iD5 Multi-Mode Microplate Reader
SpectraMax iD3
SpectraMax iD5
SpectraMax iD5 Multi-Mode Microplate Reader
Viewing Results on SpectraMax iD3 Multi-Mode Microplate Reader
Starting a Plate Read using SpectraMax iD3 Multi Mode
Personalizing Your SpectraMax iD3
Viewing Results on the SpectraMax iD5
Starting a Plate Read on the Spectramax iD5
Personalizing Your SpectraMax iD5
Related Products & Services of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Featured Applications
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the…
Read More
DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can…
Read More
HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance…
Read More
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level…
Read More
Western Blot Detection
Western blotting is among the most common methods employed for the detection and quantitation of…
Read MoreReady to get started?
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