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NFC-based personalization and enhanced touchscreen workflows provide walk-up usability

The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.

With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.

  • Personalize workflows

    Personalize workflows

    Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.

  • Simplify tasks

    Simplify tasks

    The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.

  • Reduce background noise

    Reduce background noise

    The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.

SpectraMax iD3


  • QuickSync

    The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.

  • Flexible temperature control

    Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.

  • Automatic filter identification

    Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.

  • Enhanced security

    The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.

  • Hybrid optical system

    The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.

  • Flash assays captured with ease

    The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.

Proven GxP solutions to assure data integrity and compliance

Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory.

  • Best-in-class microplate readers and washers support all your assay needs
  • IQ/OQ/PM services preserve instrument documentation in a digital and compliant format
  • Software installation services verify and document that required components are installed to operational specifications
  • Software validation service supports FDA 21 CFR Part 11 guidelines
  • Validation plates test the performance of your microplate reader using traceable materials for reliable results
GxP compliance solutions for GMP-GLP labs


Applications of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers

  • Absorbance


    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    Cell Viability

    Cell Viability

    Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.

    Read Application Note 

  • DNA/RNA Quantitation

    DNA/RNA Quantitation

    The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.

    Read Application Note 



    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

  • Fluorescence


    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

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    HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
    (This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

    Read Application Note 

  • Luminescence


    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

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    Reporter Gene Assay

    SpectraMax DuoLuc Reporter Assay High Resolution

    Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.

    Read Application Note 



    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

    Learn more  

    Western Blot

    Western Blot Detection

    Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. We’ll also introduce you to a novel, first-of-its-kind western blot detection system on a multi-mode microplate reader.

    Learn more 

Specifications & Options of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers


*Using lowest settings and speed read when available.

Resources of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers

Videos & Webinars
SpectraMax iD3 Multi-Mode Microplate Reader

Overview of SpectraMax iD3 Multi-Mode Microplate Reader

SpectraMax iD3 Tutorial Video - Getting to know your new SpectraMax iD3

Getting to know your new SpectraMax iD3

SpectraMax iD5 Multi-Mode Microplate Reader

New SpectraMax iD5 Multi-Mode Microplate Reader

SpectraMax iD5 Microplate Reader

SpectraMax iD5 Multi-Mode Microplate Reader

Week 5 Video Thumbnail

Urban myths of microplate readers: Read-Copy-Paste-Analyze. Repeat... Sound familiar?

Week 4 Video Thumbnail

Urban myths of microplate readers: Beyond the basics - real time, resolving time and transferring energy

Week 3 Video Thumbnail

Urban myths of microplate readers: “Optimization? But the manual says I need to be excited at 490nm!"

 Week 1 Video Thumbnail

Urban myths of microplate readers: OD, RFU or RLU - What exactly are they and why bigger is not always better!

Week 2 Video thumbnail

Urban myths of microplate readers: Which microplate reader? Decisions, decisions and how to be less confused!

Microplate Readers from ELISA to AlphaScreen

Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen

Microplate based Detection

Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening

ELISA endpoint protocol

How to configure an ELISA endpoint protocol

SpectraMax iD3

SpectraMax iD5

SpectraMax iD3 Tutorial Video - Viewing Results

Viewing Results on SpectraMax iD3 Multi-Mode Microplate Reader

SpectraMax iD3 Tutorial Video - Starting a Plate Read

Starting a Plate Read using SpectraMax iD3 Multi Mode

SpectraMax iD3 Tutorial Video - Personalizing your SpectraMax iD3

Personalizing Your SpectraMax iD3

SpectraMax iD5 Tutorial Video - Viewing results on the SpectraMax iD5

Viewing Results on the SpectraMax iD5

SpectraMax iD5 Tutorial Video - Starting a plate read on the Spectramax iD5

Starting a Plate Read on the Spectramax iD5

SpectraMax iD5 Tutorial Video - Personalizing your SpectraMax iD5

Personalizing Your SpectraMax iD5

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