SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Multi-user microplate readers with a large touchscreen and NFC functionality
NFC-based personalization and enhanced touchscreen workflows provide walk-up usability
The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.
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Personalize workflows
Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.
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Simplify tasks
The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.
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Reduce background noise
The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.
SpectraMax iD3
Features
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QuickSync
The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.
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Flexible temperature control
Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.
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Automatic filter identification
Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.
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Enhanced security
The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.
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Hybrid optical system
The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.
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Flash assays captured with ease
The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.
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Application Note
Optimized HTRF settings for SpectraMax multi-mode microplate readers
Optimized HTRF settings for SpectraMax multi-mode microplate readers
HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers.
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Application Note
Optimized settings for Transcreener FI Assays on SpectraMax iD3 and iD5 microplate readers
Optimized settings for Transcreener FI Assays on SpectraMax iD3 and iD5 microplate readers
Transcreener® HTS is a universal, highthroughput, biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes. Many of these enzymes…
Applications of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
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Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.
DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.
DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.
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HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
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Western Blot Detection
Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. As well as an introduction to a novel, first-of-its-kind western blot detection in a multi-mode microplate reader platform.
Western Blot Detection
Western blotting is a popular technique used for protein detection and quantitation. Learn about the various techniques used to detect proteins on western blot membranes including colorimetric, fluorescence and chemiluminescence. As well as an introduction to a novel, first-of-its-kind western blot detection in a multi-mode microplate reader platform.
Specifications & Options of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Resources of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
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Application Note
Optimized HTRF settings for SpectraMax multi-mode microplate readers
Optimized HTRF settings for SpectraMax multi-mode microplate readers
HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers.
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Application Note
Optimized settings for Transcreener FI Assays on SpectraMax iD3 and iD5 microplate readers
Optimized settings for Transcreener FI Assays on SpectraMax iD3 and iD5 microplate readers
Transcreener® HTS is a universal, highthroughput, biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes. Many of these…
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Application Note
Detect GPCR activity with the cAMP-Gs HiRange HTRF assay
Detect GPCR activity with the cAMP-Gs HiRange HTRF assay
HTRF® is a versatile technology developed by Cisbio for detecting biomolecular interactions.
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Application Note
Measure cancer cell viability using a homogeneous, stable luminescence assay
Measure cancer cell viability using a homogeneous, stable luminescence assay
Luminescent cell viability assays offer sensitivity and an easy workflow for monitoring the effects of various experimental conditions.
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Application Note
HTRF profiling services on SpectraMax iD5 Multi-Mode Microplate Reader
HTRF profiling services on SpectraMax iD5 Multi-Mode Microplate Reader
Cisbio’s Custom Solutions Laboratory team has worked in collaboration with Molecular Devices to generate relevant data promoting the expert profiling service activity completed by…
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Application Note
Measure p53-MDM2 protein interaction with NanoBRET technology
Measure p53-MDM2 protein interaction with NanoBRET technology
We describe validation of the SpectraMax® iD5 reader with the NanoBRET™ PPI Control Pair, consisting of the interacting protein partners p53 and MDM2.
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Application Note
Streamline BCA-based protein quantitation on the SpectraMax iD5 reader
Streamline BCA-based protein quantitation on the SpectraMax iD5 reader
Learn how to quantitate a cellular protein sample with BCA protein assay kits on the SpectraMax® iD5 Multi-Mode Microplate Reader.
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Application Note
Faster ELISA results with CatchPoint SimpleStep ELISA kits
Faster ELISA results with CatchPoint SimpleStep ELISA kits
CatchPoint SimpleStep ELISA kits give users a 90-minute ELISA workflow, a nearly two-thirds reduction in time compared to conventional ELISAs.
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Application Note
Measure intracellular reactive oxygen species using far-red fluorescence
Measure intracellular reactive oxygen species using far-red fluorescence
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen resulting from aerobic cellular metabolism. These chemical species are involved in cellular processes…
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Application Note
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most…
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Application Note
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader
In this application note we show how the SpectraMax® Paradigm® Multi-Mode Microplate Reader is used to perform robust, no-wash cytokine assays with excellent Z’ factors. HTRF® is a…
Flyer
Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay
Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay
ATP standards ranging from 1 μM to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). 50 μL of standard was pipetted into the wells of a white 96…
Brochure
SpectraMax iD5 Multi-Mode Microplate Reader
SpectraMax iD5 Multi-Mode Microplate Reader
Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help…
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Application Note
Optimized settings for Transcreener TR-FRET assays on SpectraMax iD5 and i3x readers
Optimized settings for Transcreener TR-FRET assays on SpectraMax iD5 and i3x readers
Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which…
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Application Note
Determine total aflatoxin in cannabis using an ELISA method
Determine total aflatoxin in cannabis using an ELISA method
As cannabis is legalized in more states across the US, state agencies are setting their own regulations and requirements for growers, retailers, and testing laboratories. For example,…
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Application Note
Evaluate anti-inflammatory compounds using a multiparametric THP-1 cell-based assay
Evaluate anti-inflammatory compounds using a multiparametric THP-1 cell-based assay
Macrophages originate from blood monocytes that leave the circulation and penetrate various tissues where they differentiate into macrophages.
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Application Note
Measure long-term cell growth using a discontinuous kinetic reading
Measure long-term cell growth using a discontinuous kinetic reading
Many biological experiments require monitoring cell growth or measuring enzymatic changes over long periods of time (hours, days or even weeks). In addition, certain model organisms…
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Application Note
Measure intrinsic tryptophan fluorescence on the SpectraMax iD3 microplate reader
Measure intrinsic tryptophan fluorescence on the SpectraMax iD3 microplate reader
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Tryptophan, which excites maximally around 270-280 nm and has an…
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Application Note
Measuring cell health on the SpectraMax iD3 reader with cell viability assays
Measuring cell health on the SpectraMax iD3 reader with cell viability assays
Measuring cell health from the multiple vantage points of viability and apoptotic pathways can provide insight into the impacts of a variety of experimental treatments including drug…
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Application Note
Streamline the workflow for measuring IgG in cell line development
Streamline the workflow for measuring IgG in cell line development
The process of cell line development for the production of antibody-based therapeutics can vary widely amongst researchers. The ultimate goal is to find a clone that stably produces…
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Application Note
DNA and RNA absorbance measurements using SpectraMax Microplate Readers
DNA and RNA absorbance measurements using SpectraMax Microplate Readers
Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Since then, microplate measurements of DNA, RNA,…
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Application Note
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite outgrowth and is regulated by…
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Application Note
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA Boosters
Detect Inflammatory Cytokines at Picogram Levels with Sword ELISA Boosters
Traditional ELISA (enzyme-linked immunosorbent assay) methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance…
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Application Note
Connectivity and user interaction with the SpectraMax iD3 Multi-Mode Microplate Reader
Connectivity and user interaction with the SpectraMax iD3 Multi-Mode Microplate Reader
The 21st century laboratory has become a fast-paced environment characterized by frequent changes in assay technology and increased employee turnover, leaving less time for complex…
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Application Note
Detection and quantitation of protein with ScanLater Western Blot Detection System
Detection and quantitation of protein with ScanLater Western Blot Detection System
Protein detection is important for pharmaceutical and clinical research today, and western blots are among the most common methods employed for this purpose. Various techniques are…
Data Sheet
SpectraMax iD3 Multi-Mode Microplate Reader
SpectraMax iD3 Multi-Mode Microplate Reader
The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With optimized rea…
eBook
Explore cells from the inside out
Explore cells from the inside out
Study protein-protein interaction to cell cycle analysis in spheroids with patented microplate reader technologies.
Scientific Poster
Multi-parametric phenotypic THP-1 cell differentiation and cytokine secretion assay for evaluation of anti-inflammatory compounds
Multi-parametric phenotypic THP-1 cell differentiation and cytokine secretion assay for evaluation of anti-inflammatory compounds
Macrophages originate from blood monocytes that leave the circulation to differentiate into various tissues. Macrophages are involved in the detection and phagocytosis of bacteria and d…
Videos and Demos
Overview of SpectraMax iD3 Multi-Mode Microplate Reader
Getting to know your new SpectraMax iD3
New SpectraMax iD5 Multi-Mode Microplate Reader
SpectraMax iD5 Multi-Mode Microplate Reader
Accelerating study of viral infection and therapeutics with microplate-based detection and high-throughput screening
How to configure an ELISA endpoint protocol
SpectraMax iD3
SpectraMax iD5
Viewing Results on SpectraMax iD3 Multi-Mode Microplate Reader
Starting a Plate Read using SpectraMax iD3 Multi Mode
Personalizing Your SpectraMax iD3
Viewing Results on the SpectraMax iD5
Starting a Plate Read on the Spectramax iD5
Personalizing Your SpectraMax iD5
Related Products & Services of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Featured Applications
Cell Viability
Cell viability can be assessed by examining parameters such as cell membrane integrity or the…
DNA/RNA Quantitation
The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can…
HTRF
HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance…
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level…
Western Blot Detection
Western blotting is a popular technique used for protein detection and quantitation. Learn about…