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Flash and glow luminescence detection in an upgradeable platform

The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement.

  • Detect all samples

    Detect all samples

    The analog and photon counting capability provides a dynamic range of nine orders of magnitude, eliminating the need to dilute bright samples while maintaining high sensitivity for dim samples.

  • Get 2-fold improvement in speed

    Get 2-fold improvement in speed

    The 2-channel configuration is available with 0, 2, or 4 injectors. The 4-injector configuration enables a 2-fold improvement in speed for traditional flash and glow assays.

  • Automate injector workflows

    Automate injector workflows

    The autowashing injectors can be programmed to rinse the injectors following each use, reversing the direction of flow so reagents can be recovered.




  • Reduced noise

    Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and low crosstalk.

  • PMT calibration

    The automatic PMT calibration method is based on readings of 4 internal LEDs at 395, 470, 527, and 570 nm. The manual PMT calibration method is based on readings of 4 wells from the assay itself.

Applications of SpectraMax L Microplate Reader

  • Cytotoxicity


    Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a way to easily screen cytotoxicity in treated cells is critical to identifying new therapeutic treatments or understanding cellular signaling pathways that affect cell health. Common indicators of cytotoxicity include the ATP level in a cell population and integrity of cellular membranes, both of which can be measured using a variety of microplate-based assays.

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    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

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  • Luminescence


    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

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    Mycoplasma Monitoring

    Mycoplasma Monitoring

    Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of proliferation and changes in cellular responses, including gene expression. Mycoplasma cannot be detected by simply examining cell cultures under a microscope, so a variety of methods such as fluorescence- and luminescence-based assays have been developed to enable researchers to monitor for contamination.

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  • Reporter Gene Assay

    SpectraMax DuoLuc Reporter Assay High Resolution

    Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.

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Specifications & Options of SpectraMax L Microplate Reader

Resources of SpectraMax L Microplate Reader

Videos & Webinars
Discovery and Functional Fine-Mapping of Expression Quantitative Trait Loci in Primary Liver Tissue

Discovery and Functional Fine-Mapping of Expression Quantitative Trait Loci in Primary Liver Tissue

SpectraMax® L Microplate Reader

Product Product Number
SpectraMax L Microplate Reader SpectraMax L
BRET1 Filter Set (480nm/535nm) 0310-5706
BRET2 Filter Set (410nm/515nm) 0310-5707
Barcode reader 0310-5743
SpectraTest LM1 Luminescence Validation Plate 0200-6186
StakMax Handling Stacker Base Unit STAKMAX
Microplate Reader Shelf 9000-0756

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