Flash and glow luminescence detection in an upgradeable platform
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement.
Detect all samples
The analog and photon counting capability provides a dynamic range of nine orders of magnitude, eliminating the need to dilute bright samples while maintaining high sensitivity for dim samples.
Get 2-fold improvement in speed
The 2-channel configuration is available with 0, 2, or 4 injectors. The 4-injector configuration enables a 2-fold improvement in speed for traditional flash and glow assays.
Automate injector workflows
The autowashing injectors can be programmed to rinse the injectors following each use, reversing the direction of flow so reagents can be recovered.
Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and low crosstalk.
The automatic PMT calibration method is based on readings of 4 internal LEDs at 395, 470, 527, and 570 nm. The manual PMT calibration method is based on readings of 4 wells from the assay itself.
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems, such…
Applications of SpectraMax L Microplate Reader
Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a way to easily screen cytotoxicity in treated cells is critical to identifying new therapeutic treatments or understanding cellular signaling pathways that affect cell health. Common indicators of cytotoxicity include the ATP level in a cell population and integrity of cellular membranes, both of which can be measured using a variety of microplate-based assays.
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of proliferation and changes in cellular responses, including gene expression. Mycoplasma cannot be detected by simply examining cell cultures under a microscope, so a variety of methods such as fluorescence- and luminescence-based assays have been developed to enable researchers to monitor for contamination.
Reporter Gene Assay
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, typically in a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
Resources of SpectraMax L Microplate Reader
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems,…
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of…
Cytotoxicity in Cells: Easy Determination Using Lonza ViaLight Plus and ToxiLight BioAssays on the SpectraMax L Microplate Luminometer
Bioluminescent cytotoxicity assays offer the user increased detection limits, speed, and accuracy, and have been well documented. Healthy cells maintain a high constant ATP…
Reporter gene assays are used to study the expression of eukaryotic genes. In dual reporter gene assays cells are transfected with two vectors, the first containing an experimental…
Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides that have been implicated in mammalian vascular development and in disease processes involving…
Learn how SoftMax Pro 7 Data Acquisition Software provides a new data analysis features, while keeping its intuitive layout.
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. Our…
Videos and Demos
Discovery and Functional Fine-Mapping of Expression Quantitative Trait Loci in Primary Liver Tissue
SpectraMax® L Microplate Reader
|SpectraMax L Microplate Reader||SpectraMax L
|BRET1 Filter Set (480nm/535nm)||0310-5706|
|BRET2 Filter Set (410nm/515nm)||0310-5707|
|SpectraTest LM1 Luminescence Validation Plate||0200-6186|
|StakMax Handling Stacker Base Unit||STAKMAX|
|Microplate Reader Shelf||9000-0756|
Related Products & Services of SpectraMax L Microplate Reader
Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a…Read More
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein,…Read More
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures…Read More
University of Geneva uses the FlexStation 3 and SpectraMax readers for assay development and screening
Supporting a wide range of assays from enzymatic target to cell-based assays.
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