SpectraMax L Microplate Reader
Sensitive luminometer with programmable injector options for 96- and 384-well microplates
Sensitive luminometer with programmable injector options for 96- and 384-well microplates
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement.
Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and low crosstalk.
The automatic PMT calibration method is based on readings of 4 internal LEDs at 395, 470, 527, and 570 nm. The manual PMT calibration method is based on readings of 4 wells from the assay itself.
Luminescent ATP assays for cell viability and cytotoxicity provide a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatments. These assays can be done in a high-throughput format using SpectraMax® microplate readers.
Learn about the CellTiter-Glo Luminescent Cell Viability Assay and more:
Cell viability refers to the number of healthy cells in a population and can be evaluated using assays that measure enzyme activity, cell membrane integrity, ATP production, and other indicators. These methods can employ luminescent, fluorescent, or colorimetric readouts as indicators of general cell viability or even specific cellular pathways. Cytotoxicity and cell viability assays are often used to assess a drug or other treatment’s effect, and are valuable tools in the search for new therapeutics, as well as advancing our understanding of how normal cells function.
Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.
Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides that have been implicated in mammalian vascular development and in disease processes involving abnormal blood vessel growth.
Learn more about chemiluminescent ELISA method using our luminometer and data acquisition and analysis software:
Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a way to easily screen cytotoxicity in treated cells is critical to identifying new therapeutic treatments or understanding cellular signaling pathways that affect cell health. Common indicators of cytotoxicity include the ATP level in a cell population and integrity of cellular membranes, both of which can be measured using a variety of microplate-based assays.
Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a way to easily screen cytotoxicity in treated cells is critical to identifying new therapeutic treatments or understanding cellular signaling pathways that affect cell health. Common indicators of cytotoxicity include the ATP level in a cell population and integrity of cellular membranes, both of which can be measured using a variety of microplate-based assays.
Learn more about cytotoxicity in cells:
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.
Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s biomass, and microbes in the human body outnumber human cells by 10 to 1. These microorganisms provide great benefit to us and are also vital to many fields of research from medicine to alternative energy production. On the other hand, monitoring for microbes and the toxic substances they produce is necessary to ensure the safety of pharmaceutical products. Scientists whose research relies on mammalian cells must carefully monitor these cultures for unwanted microbial contaminants to ensure that their experimental results are reliable.
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of proliferation and changes in cellular responses, including gene expression
Learn how the MycoAlert Assay and MycoAlert PLUS Assay from Lonza provide a rapid and convenient way to detect viable mycoplasma in cell cultures using a luminescence microplate reader
BRET (bioluminescence resonance energy transfer) is a technique for measuring protein-protein or protein-ligand interactions that involves the interaction of a bioluminescent donor and a fluorescent acceptor.
Detection of NanoBRET signals, and analysis of the resulting data, requires sensitive instrumentation and advanced software. Register to learn more:
Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems, such as DNA damage and lipid peroxidation, resulting in cellular damage.
We demonstrate how SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay:
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.
Reporter gene assays are important tools for studying gene expression associated with the activation of cellular pathways. Cells are transfected with a plasmid containing the reporter gene and a sequence of interest, typically a promoter or other transcriptional control element. When the promoter is activated, the reporter gene is expressed and its levels can be measured.
Learn more about dual luciferase reporter gene assay:
Publications
According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. While they play an important role in generating data from a wide range o…
Publications
The pharmaceutical world is a highly regulated environment. And for good reason. As a potential drug moves through the development process – from the in vitro discovery phase, to pre…
Blog
Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
Blog
The regulations for food and drug in the United States, described in the Title 21 of the Code of Federal Regulations, and the EudraLex Annex 11 in EU, are critical in ensuring safe…
Application Note
As part of the immune response to SARS-CoV-2, infected individuals produce virus-specific antibodies that are detectable in the blood within one to three weeks after the onset of…
Blog
For over 40 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Flyer
ATP standards ranging from 1 μM to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). 50 μL of standard was pipetted into the wells of a white…
Application Note
Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most…
Customer Breakthrough
M. Yves Cambet is a researcher within the Readers, Assay Development & Screening (RE.A.D.S) Unit at the Faculty of Medicine in the Centre Medical Univerisitaire (C.M.U.) of Gene…
Data Sheet
Microplate readers from Molecular Devices are designed to provide consistent performance for many years. In keeping with best practices, reader performance should still be validated…
Application Note
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems,…
Application Note
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of…
Application Note
Bioluminescent cytotoxicity assays offer the user increased detection limits, speed, and accuracy, and have been well documented. Healthy cells maintain a high constant ATP…
Application Note
Reporter gene assays are used to study the expression of eukaryotic genes. In dual reporter gene assays cells are transfected with two vectors, the first containing an experimental…
Application Note
Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides that have been implicated in mammalian vascular development and in disease processes involving…
Application Note
Endpoint readers are prolific in the laboratory since absorbance has become the detection of choice for many applications. Examples include ELISAs for quantitation of cytokines and…
Application Note
The CellTiter-Glo assay from Promega uses luciferase enzyme, which requires ATP in order to generate light. The luminescent signal produced in the assay depends on the amount of ATP in…
Brochure
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. O…
Data Sheet
Learn more about the SpectraMax L Microplate Reader and its sensitive, reliable optics and injectors, and how it can be utilized to improve your research.
Discovery and Functional Fine-Mapping of Expression Quantitative Trait Loci in Primary Liver Tissue
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Bioluminescent cytotoxicity assays offer the user increased detection limits, speed, and accuracy, and have been well documented (Crouch et al., 1993). Healthy cells maintain a high constant ATP concentration in culture. During proliferation, the total ATP in the culture increases in line with cell number. Conversely, dying cells are unable to maintain their high ATP level, as synthesis becomes compromised, and so levels are seen to reduce. The level of ATP present within a culture is indicative of the number of viable cells present, and ATP assays have been shown to be one of the most predictive general cytotoxicity methods
Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides that have been implicated in mammalian vascular development and in disease processes involving abnormal blood vessel growth. VEGFs are expressed during embryogenesis, where deletion of even a single VEGF allele severely disrupts vasculogenesis and is embryonic lethal. VEGF165 is the most abundant and biologically active isoform of VEGF found in mammals.1 The QuantiGlo Chemiluminescent VEGF Immunoassay is a solid phase ELISA that measures VEGF165 levels in cell culture supernatants, serum, plasma, saliva, and urine. It uses the quantitative sandwich enzyme immunoassay technique in which a monoclonal antibody specific for VEGF is coated onto a microplate and standards and samples are added to the wells.2 Unbound material is washed away, and a horseradish peroxidase-linked polyclonal antibody-specific for VEGF is added to the wells.
Nuclear and optical imaging are complementary in many aspects and there would be many advantages when optical imaging probes are prepared using radionuclides rather than classic fluorophores, and when nuclear and optical dual images are obtained using single imaging probe.
| Product | Product Number |
| SpectraMax L Microplate Reader | SpectraMax L Config |
| BRET1 Filter Set (480nm/535nm) | 0310-5706 |
| BRET2 Filter Set (410nm/515nm) | 0310-5707 |
| Barcode reader | 0310-5743 |
| SpectraTest LM1 Luminescence Validation Plate | 0200-6186 |
| StakMax Handling Stacker Base Unit | STAKMAX |
| Microplate Reader Shelf | 9000-0756 |
Luminescent ATP assays for cell viability and cytotoxicity provide a sensitive and rapid way to…
Cell viability refers to the number of healthy cells in a population and can be evaluated using…
Cellular signaling allows cells to respond to their environment as well as to communicate with…
Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides that have been…
Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a…
Cytotoxicity is often measured in response to an experimental treatment or potential drug. Having a…
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein,…
Learn about luminescence detection – what it is, how it works, and the advantages of luminescence…
Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s…
Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures…
BRET (bioluminescence resonance energy transfer) is a technique for measuring protein-protein or…
Protein detection, quantitation, and analysis are central to the investigation of a wide variety of…
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes,…
Reporter gene assays enable researchers to determine whether a gene is expressed and at what level…
Reporter gene assays are important tools for studying gene expression associated with the…
SUCCESS STORY
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