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MetaMorph Microscopy Automation and Image Analysis Software

Microscopy imaging software for a wide variety of third-party microscopes
Microscope imaging software with optional microscopy analysis software
The MetaMorph® Microscopy Automation and Image Analysis Software automates acquisition, device control, and image analysis. It easily integrates dissimilar fluorescent microscope hardware and peripherals into a single custom workstation. The software offers many user-friendly application modules for biology-specific analysis. Two complementary packages, MetaFluor® software for fluorescence ratio imaging, and MetaVue® software for basic image acquisition and processing, are included in the portfolio.
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Support third-party microscopes
MetaMorph software works with many commercially available microscopes, laser launches, TIRF optics, and can be enabled on previously-installed imaging systems compatible with MetaMorph software.
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Measure fluorescence ratio imaging
MetaFluor® Fluorescence Ratio Imaging Software is designed for single or multi-wavelength intracellular ion measurements to provide greater insight to ion exchange and intracellular function.
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Document and analyze images
MetaVue™ Research Imaging System is a simple, easy-to-use software application for acquiring and processing images, performing graphics functions, and archiving and retrieving images.

Resolving Molecular Organization and Dynamics Using Localization-Based Super-Resolution Microscopy
Features
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Real-time image processing
Image process is supported by a graphics processing unit hardware acceleration. It resolves sub-cellular objects as small as 20 nm spatially and 40 nm axially.
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Multi-dimensional acquisition module
Allows for the capture of complex acquisition sequences using a flexible, guided user interface.
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Integrated morphometric analysis
Measures and categorizes objects into discreet user-definable classes based on any combination of morphometric parameters, such as shape, size, or optical density.
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Scan slide module
Automates the acquisition of multiple images and then stitches them seamlessly together. Ideal for large tissue samples, this ensures reproducibility while taking the guesswork out of tiling experiments.
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Device and camera streaming
Accelerates image capture rate and simultaneously transfers images to memory during acquisition, capturing dynamic cellular events for applications such as live cell/kinetic imaging.
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4D viewer with 3D measurements
Tools for multidimensional visualization including stacks of sequential images, multiple Z-sections, wavelengths, time points, and positions. Data can be rendered for 3D isosurface viewing and rotation.
Latest Resources
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Toxicology
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.
Resources of MetaMorph Microscopy Automation and Image Analysis Software
Customer Breakthrough
Research team reduces scan times by 88% using MetaMorph Microscopy Automation and Image Analysis Software
Research team reduces scan times by 88% using MetaMorph Microscopy Automation and Image Analysis Software
The main fields of research for the team at the Laboratory of Mucosal Barrier Pathobiology, Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School are epitheli…
Blog
Tips for running a successful live cell imaging experiment
Tips for running a successful live cell imaging experiment
There have been significant advancements in microscopy and camera technology, as well as advancements in technologies for labeling molecules of interest over the past decade. These…
Blog
Overcome the challenges of high-content cell analysis through AI/machine learning
Overcome the challenges of high-content cell analysis through AI/machine learning
Artificial intelligence (AI) is finding its way into many aspects of modern life, from autonomous vehicles to voice-powered personal assistants, and even the creation of art. But it…
Scientific Poster
Automated 3D cell-based assays using a novel flow chip system and high-content imaging
Automated 3D cell-based assays using a novel flow chip system and high-content imaging
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translation research. Significant progress has b…
eBook
Cellular Imaging Insights
Cellular Imaging Insights
Gain insights and expedite studies for 2D and 3D cellular structures using automated cellular imaging.
Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Blog
Get to know our Field Applications Scientist: Kayla Hill
Get to know our Field Applications Scientist: Kayla Hill
Kayla Hill discusses the latest trends in cellular imaging We recently hosted a webinar with our Field Applications Scientist, Kayla Hill, PhD, who explored high-content analysis…
Brochure
MetaMorph® Microscopy Automation and Image Analysis Software
MetaMorph® Microscopy Automation and Image Analysis Software
The MetaMorph® Microscopy Automation and Image Analysis Software from Molecular Devices plays a large role in this revolution. With its image acquisition, processing and analysis cap…
Customer Breakthrough
Institute of Atomic and Molecular Sciences uses MetaMorph software to study proteins at the transition zone of primary cilia
Institute of Atomic and Molecular Sciences uses MetaMorph software to study proteins at the transition zone of primary cilia
Dr. Jung-Chi Liao’s lab at Academia Sinica in Taiwan focuses on understanding the structure and function of proteins at the base of primary cilia in mammalian cells. Specifically, th…
Data Sheet
MetaXpress Software Mitotic Index Application Module
MetaXpress Software Mitotic Index Application Module
Explore Mitotic Index datasheet to get information about MetaXpress App Module designed for quantitative discrimination of mitotic & interphase cells.
Application Note
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite outgrowth and is regulated by…
Application Note
MetaXpress Software Angiogenesis Application Module
MetaXpress Software Angiogenesis Application Module
Angiogenesis is the growth of new blood vessels and is an important natural process occurring in the body, both in health and disease. Angiogenesis occurs in the healthy body for…
Application Note
Expanded fluorescence screening using QPix 400 series
Expanded fluorescence screening using QPix 400 series
Bacterial screening is a popular method used for detecting recombinant bacteria in vector-based molecular cloning. It can also be used to measure specific protein expression and…
Brochure
MetaFluor® Fluorescence Ratio Imaging Software
MetaFluor® Fluorescence Ratio Imaging Software
The MetaFluor Imaging System is designed to display raw data, ratio image, graphs of intensities, ratios & ion concentrations, & ratiometric images such as brightfield or pha…
Brochure
MetaMorph Super-Resolution Software
MetaMorph Super-Resolution Software
Explore MetaMorph Microscopy & Imaging Software used to control experimental hardware, capture images, perform localization do calculations, and display super-resolution image de…
Application Note
Synchronizing electrophysiology and imaging studies with Axon pCLAMP and MetaMorph Software
Synchronizing electrophysiology and imaging studies with Axon pCLAMP and MetaMorph Software
Live cell research is increasingly in need of employing complementary data acquisition simultaneously to describe intracellular processes. Simultaneous recordings with both imaging and…


Immunology and Vaccine Development Workflow

Hybridoma Workflow

Resolving Molecular Organization and Dynamics Using Localization-Based Super-Resolution Microscopy

Real-Time Single-Molecule Based Super-Resolution Microscopy Reconstruction: Theoretical and Practical Insight
Number of Citations*: 27,600
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
- Dated: Aug 20, 2011Publication Name: Journal of Nanoparticle Research
Cerium oxide and platinum nanoparticles protect cells from oxidant-mediated apoptosis
Catalytic nanoparticles represent a potential clinical approach to replace or correct aberrant enzymatic activities in patients. Several diseases, including many blinding eye diseases, are promoted by excessive oxidant stress due to reactive oxygen species (ROS). Cerium oxide and platinum nanoparticles represent two potentially therapeutic… View moreCatalytic nanoparticles represent a potential clinical approach to replace or correct aberrant enzymatic activities in patients. Several diseases, including many blinding eye diseases, are promoted by excessive oxidant stress due to reactive oxygen species (ROS). Cerium oxide and platinum nanoparticles represent two potentially therapeutic nanoparticles that de-toxify ROS. In the present study, we directly compare these two classes of catalytic nanoparticles. Cerium oxide and platinum nanoparticles were found to be 16 ± 2.4 and 1.9 ± 0.2 nm in diameter, respectively. Using surface plasmon-enhanced microscopy, we find that these nanoparticles associate with cells. Furthermore, cerium oxide and platinum nanoparticles demonstrated superoxide dismutase catalytic activity, but did not promote hemolytic or cytolytic pathways in living cells. Importantly, both cerium oxide and platinum nanoparticles reduce oxidant-mediated apoptosis in target cells as judged by the activation of caspase 3. The ability to diminish apoptosis may contribute to maintaining healthy tissues.
- Dated: Aug 30, 2007Publication Name: Journal of Neuroscience Methods
High throughput quantification of cells with complex morphology in mixed cultures
Automated image-based and biochemical assays have greatly increased throughput for quantifying cell numbers in in vitro studies. However, it has been more difficult to automate the counting of specific cell types with complex morphologies in mixed cell cultures. We have developed a fully automated, fast, accurate and objective method for the… View moreAutomated image-based and biochemical assays have greatly increased throughput for quantifying cell numbers in in vitro studies. However, it has been more difficult to automate the counting of specific cell types with complex morphologies in mixed cell cultures. We have developed a fully automated, fast, accurate and objective method for the quantification of primary human GFAP-positive astrocytes and CD45-positive microglia from images of mixed cell populations. This method, called the complex cell count (CCC) assay, utilizes a combination of image processing and analysis operations from MetaMorph™ (Version 6.2.6, Molecular Devices). The CCC assay consists of four main aspects: image processing with a unique combination of morphology filters; digital thresholding; integrated morphometry analysis; and a configuration of object standards. The time needed to analyze each image is 1.82 s. Significant correlations have been consistently achieved between the data obtained from CCC analysis and manual cell counts. This assay can quickly and accurately quantify the number of human astrocytes and microglia in mixed cell culture and can be applied to quantifying a range of other cells/objects with complex morphology in neuroscience research.
Contributors: Pritika J.Narayan, Hannah M.Gibbons, Edward W.Mee, Richard L.Faull, Michae Dragunow
Go to article - Dated: Mar 21, 2007Publication Name: Journal of Neuroscience
Functional Neural Development from Human Embryonic Stem Cells: Accelerated Synaptic Activity via Astrocyte Coculture
How a naive human neuroepithelial cell becomes an electrophysiologically active neuron remains unknown. Here, we describe the early physiological development of neurons differentiating from naive human embryonic stem (hES) cells. We found that differentiating neuronal cells progressively decrease their resting membrane potential, gain… View moreHow a naive human neuroepithelial cell becomes an electrophysiologically active neuron remains unknown. Here, we describe the early physiological development of neurons differentiating from naive human embryonic stem (hES) cells. We found that differentiating neuronal cells progressively decrease their resting membrane potential, gain characteristic Na+ and K+ currents, and fire mature action potentials by 7 weeks of differentiation.
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SUCCESS STORY
Research team reduces scan times by 88% using MetaMorph Microscopy Automation and Image Analysis Software
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…