Overview of FLIPR Potassium Assay Kit

The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+ conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.

  • Functional measurement of potassium channel activity in a cell-based assay
  • Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow
  • Expanded signal window compared to non-homogenous assay
  • Rapid procedure with less hands‐on time

Data of FLIPR Potassium Assay Kit

  • Comparison of FLIPR Potassium Assay Kit results

    Potassium Kits Comparison

    Comparison of FLIPR Potassium Assay Kit results to a competitor kit.

  • Signal Window Comparsion

    Comparison of signal dynamic range between the FLIPR Potassium Assay Kit and a competitor kit. Negative control is 4 μM terfenadine; positive control is buffer. The Z’ factor for the FLIPR Potassium Assay Kit = 0.85 and n = 32 compared to the competitor Z’ factor = 0.64 and n = 30.

    Comparison of FLIPR Potassium Assay Kit dynamic range

  • Concentration-dependent inhibition of hERG channel

    hERG Channel Inhibition

    Concentration-dependent inhibition of hERG channel by reference compounds.

  • Optimization of hERG Channel Stimulant

    Cells were incubated with dye and the stimulant buffers were added during detection on the FLIPR Tetra System. The concentration-dependent response of signal was characterized under different conditions. Optimal signal was obtained from the combination of 1 mM Tl+ and 10 mM K+ (final concentration) stimulant buffer diluted in chloride-free buffer.

    Optimization of hERG channel stimulant

Technology of FLIPR Potassium Assay Kit

  • FLIPR Potassium Assay Kit Mechanism

    Assay Kit Mechanism

    The assay exploits the permeability of thallium (Tl+) for potassium (K+) channels. The cells are incubated with an AM ester form of a fluorescent thallium sensitive dye. Intracellular esterases cleave off the AM group trapping the dye within the cells. Cells are then stimulated with either a mixture of K+ and Tl+ or a ligand in the presence of Tl+. The increase in fluorescent signal represents the influx of Tl+ into the cell specifically through the potassium channel and becomes a measure of potassium channel functional activity. Background is reduced by the masking solution.

Ordering Options of FLIPR Potassium Assay Kit

FLIPR Potassium Assay Kit

Explorer Kit

  • (10) Vials of Component A
  • (1) Bottle of Component B* assay buffer HBSS + Ca2+, Mg2+ and 20 mM HEPES, pH 7.4
  • (10) Vials of Component C
  • (1) Bottle of 5X chloride-free assay buffer
  • (1) Bottle of potassium sulfate solutin (200 mM)
  • (1) Bottle of thallium sulfate solution (50 mM)

Each kit contains enough material for ten (10) 96-well or 384-well microplates. Each vial is sufficient for one (1) microplate experiment.

#R8222

$835.00 USD

1

Bulk Kit

  • (10) Vials of Component A
  • (10) Vials of Component C
  • (1) Bottle of 5X chloride-free assay buffer
  • (1) Bottle of potassium sulfate solutin (200 mM)
  • (1) Bottle of thallium sulfate solution (50 mM)

Each kit contains enough material for one hundred (100) 96-well or 384-well microplates. Each vial is sufficient for ten (10) microplate experiments.

#R8223

$5,015.00 USD

1

Evaluation Kit

  • (2) Vials of Component A
  • (1) Bottle of Component B* assay buffer HBSS + Ca2+, Mg2+ and 20 mM HEPES, pH 7.4
  • (2) Vials of Component C
  • (1) Bottle of 5X chloride-free assay buffer
  • (1) Bottle of potassium sulfate solutin (200 mM)
  • (1) Bottle of thallium sulfate solution (50 mM)

Each kit contains enough material for two (2) 96-well or 384-well microplates. Each vial is sufficient for one (1) microplate experiment.

#R8330

$412.00 USD

1

Resources of FLIPR Potassium Assay Kit

Videos and Demos

Developing an in vitro Tl+ flux assay for potassium channel

Developing an in Vitro Tl+ Flux Assay for Potassium Channel Agonist Identification

Compatible Products & Services of FLIPR Potassium Assay Kit

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.