Overview of FLIPR Potassium Assay Kit
The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.
- Functional measurement of potassium channel activity in a cell-based assay
- Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow
- Expanded signal window compared to non-homogenous assay
- Rapid procedure with less hands‐on time