Overview of FLIPR Potassium Assay Kit
The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+ conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.
- Functional measurement of potassium channel activity in a cell-based assay
- Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow
- Expanded signal window compared to non-homogenous assay
- Rapid procedure with less hands‐on time
Latest Resources
Data of FLIPR Potassium Assay Kit
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Potassium Kits Comparison
Comparison of FLIPR Potassium Assay Kit results to a competitor kit.
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Signal Window Comparsion
Comparison of signal dynamic range between the FLIPR Potassium Assay Kit and a competitor kit. Negative control is 4 μM terfenadine; positive control is buffer. The Z’ factor for the FLIPR Potassium Assay Kit = 0.85 and n = 32 compared to the competitor Z’ factor = 0.64 and n = 30.
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hERG Channel Inhibition
Concentration-dependent inhibition of hERG channel by reference compounds.
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Optimization of hERG Channel Stimulant
Cells were incubated with dye and the stimulant buffers were added during detection on the FLIPR Tetra System. The concentration-dependent response of signal was characterized under different conditions. Optimal signal was obtained from the combination of 1 mM Tl+ and 10 mM K+ (final concentration) stimulant buffer diluted in chloride-free buffer.
Technology of FLIPR Potassium Assay Kit
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Assay Kit Mechanism
The assay exploits the permeability of thallium (Tl+) for potassium (K+) channels. The cells are incubated with an AM ester form of a fluorescent thallium sensitive dye. Intracellular esterases cleave off the AM group trapping the dye within the cells. Cells are then stimulated with either a mixture of K+ and Tl+ or a ligand in the presence of Tl+. The increase in fluorescent signal represents the influx of Tl+ into the cell specifically through the potassium channel and becomes a measure of potassium channel functional activity. Background is reduced by the masking solution.
Resources of FLIPR Potassium Assay Kit
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
Scientific Poster
In Vitro Potency Assessment of hERG Inhibitors: Cell-Based Thallium-Sensitive Fluorescence Assay vs. Automated Electrophysiology
In Vitro Potency Assessment of hERG Inhibitors: Cell-Based Thallium-Sensitive Fluorescence Assay vs. Automated Electrophysiology
Explore this poster to see the performance of thallium-sensitive fluorescence dye-based assay on the FLIPR Tetra compared to that obtained by electrophysiological recording technique…
Application Note
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FLIPR Tetra System
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FLIPR Tetra System
Drug-induced inhibition of the human ether-à-go-go-related gene (hERG) ion channel has been related to the susceptibility of patients to potentially fatal ventricular tachyarrhythmia,…
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Application Note
Development of a cell-based potassium-chloride transporter assay using the FLIPR Potassium Assay Kit
Development of a cell-based potassium-chloride transporter assay using the FLIPR Potassium Assay Kit
The FLIPR Potassium Assay Kit can be used to measure the functional activity of the hKCC2 cation-chloride cotransporter using a homogeneous, no-wash protocol. The assay kit displays a…
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Scientific Poster
A Novel Homogenous Potassium Ion Channel Assay for High-Throughput Screening
A Novel Homogenous Potassium Ion Channel Assay for High-Throughput Screening
Ion channels are a class of membrane proteins that mediate the movement of charged ions across the cell membrane. Potassium channels constitute the largest and most diverse group of…
Publications
Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes show promise for screening during early drug development. Here, we tested a hypothesis that in vitro assessment of m…
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Publications
Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells
Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells
A large percentage of drugs fail in clinical studies due to cardiac toxicity; thus, development of sensitive in vitro assays that can evaluate potential adverse effects on cardiomyoc…
Developing an in Vitro Tl+ Flux Assay for Potassium Channel Agonist Identification
