Fura-2 QBT Calcium Kit

The Fura-2 QBT Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2. Leveraging our proprietary masking technology with the industrystandard Fura-2 ratiometric calcium indicator, researchers can:

  • See largest Fura-2 signal window available
  • Eliminate wash artifacts and increase throughput with homogeneous assay
  • Minimize the impact of uneven dye loading and leakage on results
  • Interrogate low-density, weakly or non-adherent cells using no-wash protocol
  • Assay on FlexStation® 3 and FLIPR® Tetra Systems
  • Reference thousands of citations where Fura-2 has been used for cell lines and target

Data of Fura-2 QBT Calcium Kit

  • Fura2 CHO M1 cells AGONISM

    Agonism of the Muscarinic M1 Receptor on CHO M1 Cells

    On the FlexStation® 3 Multi-Mode Microplate Reader, carbachol was used to stimulate the muscarinic M1 receptor on CHO M1 cells to generate concentration response curves (CRC), comparing Fura-2 QBT Dye to the BD Kit and the traditional Fura-2 wash protocol. EC50 values were similar for all methods, and were within range of published values.

  • Antagonism of the Muscarinic M1 Receptor on CHO M1 Cells

    On the FlexStation 3 Multi-Mode Microplate Reader, 50 nM carbachol was used as agonist challenge versus an antagonist CRC of atropine. This experiment Fura-2 QBT Calcium Kit provided the largest signal window and most robust Z-factors at EC80.

    Fura2 CHO M1 cells ANTAGONISM

  • Fura2 H1 cells AGONISM

    Agonism of Endogenous H1 Receptor on HeLa Cells

    Histamine was used to stimulate the endogenous histamine receptor on HeLa cells. Assay was measured using the FLIPR Tetra® High-Throughput Cellular Screening System with UV LEDs. Generated concentration response curves (CRC) comparing Fura-2 QBT Dye to the BD Ratiometric Calcium Kit and traditional Fura-2 wash protocol. EC50 values were within a half log while the Z-factor at EC80 was the largest with the Fura-2 QBT Calcium Kit.

  • Antagonism of Endogenous H1 Receptor on HeLa Cells

    Assay was measured using the FLIPR Tetra High-Throughput Cellular Screening System with UV LEDs and used similar experimental setup and system settings in Figure 3. 40 nM histamine was used as agonist challenge versus an antagonist CRC of pyrilamine.

    Fura2 H1 cells ANTAGONISM

Technology of Fura-2 QBT Calcium Kit

  • Fura-2 QBT Calcium Assay Kit Mechanism

    Assay Kit Mechanism

    Fura-2 QBT Calcium Kit utilizes Fura-2, AM a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation, where lipophilic blocking groups are cleaved resulting in a negatively charged fluorescent dye that stays within the cell. Upon target activation, intracellular calcium is released, binding with the dye, thereby increasing fluorescence intensity. This kit utilizes an extracellular masking technology to block background fluorescence and increase your assay's signal window, and decrease assay steps.

  • Calcium Flux Signal Change

    Calcium-Flux signal change

    Calcium-bound Fura-2 is excited at 340/510 nm and the unbound form at 380/510 nm. Upon calcium-flux, the cytoplasm's calcium concentration increases, causing the fluorescence intensity at 340/510 nm to increase proportionately. In contrast, the 380/510 nm intensity declines in synchronization with the diminishing concentration of unbound dye. By ratioing fluorescence intensities produced by excitation at two wavelengths, artifacts associated with uneven dye distribution and cell number variation are minimized because they effect both measurements equally.

  • Fura-2 QBT Calcium Dye when Bound to Ca2+

    Spectral Shift of Fura-2 QBT Calcium Dye When Bound to Ca2+

    Fura-2 QBT™ Calcium Dye is excited at 340nm and 380 nm, with emission at 510 nm. Upon calcium flux, one can measure changes in intracellular calcium concentration.

Ordering Options of Fura-2 QBT Calcium Kit

Fura-2 QBT Calcium Kit

Evaluation Kit

  • 1 plate x 2 vials each

#R6139

$154.00 USD

1

Explorer Kit

  • 1 plate x 10 vials + Component B buffer

#R8197

$515.00 USD

1

Bulk Kit

  • 10 plates x 10 vials

 

Compatible with 96- and 384-well plate formats.

#R8198

$3,439.00 USD

1

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.