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EarlyTox Cell Viability Assay Kits
The EarlyTox™ cell viability assay kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimized for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterization of a full concentration-response profile of test compounds.
EarlyTox Cell Viability Assay Kits
Cell viability assays are critical to a broad spectrum of research areas ranging from investigation into the mechanisms of cell death to the development of new therapeutics targeting apoptosis in diseases. One of the most popular detection technologies for cell viability is a fluorescence microplate reader. The EarlyTox cell viability assay kits have been optimized for highly sensitive and accurate microplate detection which provides a complete solution for monitoring cell viability.
- Optimal data quality for microplate reader assay
- Simplified, homogeneous experimental protocol
- Preconfigured SoftMax Pro Software protocols
EarlyTox™ Live/Dead Assay Kit
This kit enables the detection of both the live and dead populations of mammalian cells based on the integrity of the cell membrane using a fluorescence microplate reader. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted into the fluorescent calcein by intracellular esterases. The number of live cells is therefore indicated by the intensity of green fluorescence in the cytosol, with excitation at 495 nm and emission at 530 nm. EthD-III is virtually non-fluorescent and impermeant to an intact plasma membrane. In the event of compromised cell membrane integrity that is associated with cell death, EthD-III enters cells and binds to nucleic acids, resulting in bright red fluorescence in dead cells, with excitation at 530 nm and emission at 645 nm.
EarlyTox™ Live Cell Assay Kit
This kit enables the detection of the live population of mammalian cells using a fluorescence microplate reader based on the integrity of the cell membrane and the intracellular esterase activity. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases. The number of live cells is therefore indicated by the intensity of green fluorescence in the cytosol, with excitation at 495 nm and emission at 530 nm. The low cellular toxicity of calcein AM also enables long-term cell tracing and cell proliferation assay.
EarlyTox™ Caspase-3/7 R110 Assay Kit
This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)2-R110 contains two DEVD consensus target sequence and is completely hydrolyzed in cell lysate by the enzymes. Hydrolysis of both DEVD peptides releases the green fluorescent dye R110, resulting in a substantial fluorescence increase, with excitation at 490 nm and emission at 520 nm. The assay kit includes the competitive caspase-3/7 inhibitor Ac-DEVD-CHO for use as a negative control. R110 is also provided in the kit for generating a standard curve, which can be used for quantifying caspase-3 activity.
EarlyTox™ Caspase-3/7 NucView 488 Assay Kit and EarlyTox™ Caspase-3/7-D NucView 488 Assay Kit
This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (formulated in DMSO or phosphate-buffered saline (PBS), for EarlyTox Caspase-3/7-D NucView 488 Assay Kit and EarlyTox Caspase-3/7 NucView 488 Assay Kit, respectively) is used to detect caspase-3/7 activity within intact cells without interfering with caspase activity. The substrate consists of a fluorogenic DNA dye that is coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate permeates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus and stains DNA with bright green fluorescence, with excitation at 500 nm and emission at 530 nm. NucView 488 staining is formaldehyde-fixable and compatible with subsequent immunostaining. The assay kit includes an optional masking reagent to reduce background fluorescence when necessary. The PBS formulation is recommended for cells that are sensitive to DMSO.
EarlyTox™ Glutathione Assay Kit
This kit enables the detection of an early apoptosis event based on the measurement of the glutathione content in live mammalian cells using a fluorescence microplate reader. Diminished cellular glutathione (GSH), which is an important anti-oxidant in mammalian cells, occurs at the early stages of mitochondria-associated apoptosis because of GSH efflux. This kit uses monochlorobimane (MCB), a cell permeant dye that has a high affinity for GSH, to detect the cellular GSH level in an apoptotic event. The unreacted dye is almost non-fluorescent. After reaction of the dye with GSH, catalyzed by endogenous glutathione-S-transferase (GST) enzymes, the dye fluoresces blue, with excitation at 394 nm and emission at 490 nm. The intensity of the fluorescence signal generated from the assay reflects the amount of GSH that is present in the cells.
Data of EarlyTox Cell Viability Assay Kits
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Representative Data from SpectraMax Microplate Readers
Data collected using HeLa cells. (A) Live/Dead: Cell viability as measured by the green (live)/red (dead) fluorescence ratio; (B) Caspase-3/7 R110: Cell apoptosis as measured by the caspase 3/7 activity; (C) Glutathione: Cell apoptosis as measured by the glutathione content; D&E: Caspase-3/7 NucView 488. The EarlyTox Caspase-3/7 NucView 488 Assay Kits are also applicable to fluorescence imaging, as the nuclei of intact apoptotic cells are labeled per their caspase activity; (D) Assessment of caspase 3/7 activity on plate reader; (E) Assessment of caspase 3/7 using fluorescence imaging.
Ordering Options of EarlyTox Cell Viability Assay Kits
EarlyTox Live/Dead Assay Kit
Explorer Kit - PN: R8340
- (1) vial of Calcein AM (4 mM, 30 µL)
- (1) vial of EthD-III (2 mM, 60 µL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
#R8340
Bulk Kit - PN: R8341
- (3) vials of Calcein AM (4 mM, 50 µL)
- (2) vials of EthD-III (2 mM, 150 µL)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
#R8341
EarlyTox Live Cell Assay Kit
Explorer Kit - PN: R8342
- (1) vial of Calcein AM (4 mM, 30 µL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
#R8342
Bulk Kit - PN: R8343
- (3) vials of Calcein AM (4 mM, 50 µL)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
#R8343
EarlyTox Glutathione Assay Kit
Explorer Kit - PN: R8344
- (1) vial of MCB (10 mM, 100 µL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
#R8344
Bulk Kit - PN: R8345
- (1) vial of MCB (10 mM, 500 µL)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
#R8345
EarlyTox Caspase-3/7 R110 Assay Kit
Explorer Kit - PN: R8346
- (1) vial of Rhodamine 110 (80 µM, 1 mL)
- (2) vials of caspase-3 inhibitor (5 mM, 20 µL)
- (2) vials of DEVD-R110 (2 mM, 500 µL)
- (2) vials of lysis buffer (10 mL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
#R8346
EarlyTox Caspase-3/7-D NucView 488 Assay Kit
Explorer Kit - PN: R8348
- (1) vial of NucView 488 Caspase-3 Substrate in DMSO (1 mM, 200 µL)
- (2) vials of masking reagent (1 vial/plate)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
#R8348
EarlyTox Caspase-3/7 NucView 488 Assay Kit
Explorer Kit - PN: R8350
- (1) vial of NucView 488 Caspase-3 Substrate in PBS (1 mM, 200 µL)
- (2) vials of masking reagent (1 vial/plate)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
#R8350
Bulk Kit - PN: R8351
- (1) vial of NucView 488 Caspase-3 Substrate in PBS (1 mM, 1,000 µL)
- (1) vial of masking reagent (1 vial/10 plates)
Each kit contains enough material for ten (10) 96-well or 384-well microplates.
#R8351
Resources of EarlyTox Cell Viability Assay Kits
Flyer
Assay kits for the ImageXpress Pico system
Assay kits for the ImageXpress Pico system
These assay kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and apoptosis events using mammalian cells. The kits employ a n…
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
Data Sheet
EarlyTox Cell Viability Assay Kits
EarlyTox Cell Viability Assay Kits
This datasheet explores EarlyTox Cell Viability Assay Kits which include a family of six reagents for characterizing cell viability, cell proliferation, & various apoptosis event…
Application Note
Measuring reactive oxygen species with SpectraMax microplate readers
Measuring reactive oxygen species with SpectraMax microplate readers
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems,…
Application Note
EarlyTox Glutathione Assay Kit on SpectraMax Fluorescence Microplate Readers
EarlyTox Glutathione Assay Kit on SpectraMax Fluorescence Microplate Readers
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as embryonic development, as well as diseases including cancer and neurodegenerative…
Application Note
EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
Cell viability assays are often performed to assess the effects of a variety of treatments, including drug candidates, pathway activators and inhibitors, and reporter genes. One of the…
Application Note
EarlyTox Caspase-3/7 NucView 488 Assay Kit on the SpectraMax i3x Multi-Mode Detection Platform
EarlyTox Caspase-3/7 NucView 488 Assay Kit on the SpectraMax i3x Multi-Mode Detection Platform
The EarlyTox Caspase-3/7 NucView 488 Assay Kits enable detection of apoptosis in intact cell populations through use of NucView 488 Caspase-3 substrate. This substrate consists of a…
Application Note
Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
Cell viability assays are utilized in a wide variety of research areas ranging from examining the mechanisms of cell death and cell proliferation to evaluating the effects of cytotoxic…
Application Note
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
Apoptosis is an important mechanism signaling programmed death of cells in normal processes such as embryonic development, as well as in diseases including cancer and neurodegenerative…
Application Note
Evaluation of GM-CSF and TNFα-induced apoptosis with EarlyTox Cell Viability Assay Kits
Evaluation of GM-CSF and TNFα-induced apoptosis with EarlyTox Cell Viability Assay Kits
EarlyTox Cell Viability Kits are a family of reagents for assessing a spectrum of cellular conditions, including viability and various apoptotic events. These reagents are designed for…
Application Note
EarlyTox Caspase-3/7 R110 Assay Kit on SpectraMax Fluorescence Microplate Readers
EarlyTox Caspase-3/7 R110 Assay Kit on SpectraMax Fluorescence Microplate Readers
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as embryonic development, as well as diseases including cancer and neurodegenerative…
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How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…