Tutorials & Videos

Multi-parameter in vitro assessment of compound effects on cardiomyocyte physiology using induced pluripotent stem cells

Early detection and elimination of new chemical entities with cardiac or hERG liability could substantially improve drug safety and minimize the cost of development. In this webinar, we will highlight cardiac toxicity using induced pluripotent stem cells (iPSCs).

Learning Objectives:

- Determine the effects of pharmacological compounds and other chemicals on the beating rate and profiles of cardiomyocytes using the FLIPR Tetra® system and ImageXpress® Micro system. Assay data is characterized from 2D and 3D spheroid cardiomyocyte cultures. The assays employ calcium sensitive dyes to monitor changes in intracellular Ca2+ fluxes synchronous with cell beating which allows monitoring of beat rate, amplitude, and other parameters.

- Identify methods for multiparametric analysis and ranking of compounds for potential cardiotoxic hazards. This methodology is well suited for testing environmental chemicals and early safety testing in drug development prior to clinical studies.

Multi Parameter in Vitro Assessment of on Cardiomyocyte Physiology using Stem Cells