RiboPro use the SpectraMax iD3 to advance research into life-changing mRNA therapeutics
COMPANY/UNIVERSITY
RiboPro
TEAM MEMBERS
Sander van Asbeck
Marc Penders
PRODUCTS USED
SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
The Challenge
RiboPro is a spin-off of Dutch biotechnology company Mercurna. Based in The Netherlands, the RiboPro team design and produce high-quality messenger RNA (mRNA) for academic and industrial researchers. Their mission is to accelerate the research and drug development of as many researchers as possible with their potent technology. As specialists in mRNA and drug delivery, they continuously strive to improve mRNA-based products for the benefit of their customers worldwide.
RNA is a great research tool and can be a powerful therapeutic. To deliver engineered mRNA therapeutics into cells, the team at RiboPro use a proprietary lipid nanoparticle (LNP)-based delivery vehicle. Researchers needed to be able to determine the efficiency and toxicity of their LNP and mRNA formulations so required a reliable ‘workhorse’ instrument to perform various assays.
The Solution
The team use the SpectraMax iD3 Multi-Mode Microplate Reader to perform luciferase assays to determine the performance of new LNP formulations. They also use it for cell viability assays and immunogenicity ELISAs to determine the (reduced) toxicity of both the mRNA and LNP formulations, as well as to measure the concentrations of RNA and DNA during their mRNA synthesis process. As such, it has become a crucial piece of equipment for the group. In the photo above, Marc Penders of RiboPro is using the SpectraMax iD3 for a lipid oxidation assay.
SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
Products Used
The SpectraMax® iD3 Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Built-in near-field communication (NFC) functionality enables you to pull up your custom protocols with a single tap, saving you precious time better spent on your research. It features a large, high-resolution touchscreen interface with an embedded software package allowing you to set up custom protocols, take advantage of preloaded protocols, and run your experiment without the need for a dedicated computer workstation.
With optimized reagents and industry-leading SoftMax® Pro Software, it provides an all access pass for breakthrough research.
The Results
RiboPro now use the SpectraMax iD3 Reader to measure between 10 and 50 plates per day, as well as a rapidly increasing number of RNA/DNA samples.
Sander van Asbeck (CEO Mercurna), says, “We particularly enjoy the automatic gain settings and optimization features of the SpectraMax iD3, which allow us to improve our assays and pick-up the smallest of clues to improve our products. Furthermore, with our throughput, being able to measure multiple RNA or DNA samples in one go speeds things up, further lowering the cost of our service”.