APPLICATION NOTE
Quantitative single-molecule spatial transcriptomics using the ImageXpress HCS.ai High Content Screening System
Transforming Spatial Transcriptomics with Single-Molecule Precision on the ImageXpress HCS.ai High-Content Screening System
In this application note, we demonstrate how the ImageXpress® HCS.ai High-Content Screening System, combined with IN Carta® AI-driven analysis, enables researchers to map RNA transcripts with single-molecule precision while preserving spatial and morphological context. The result is a powerful approach for uncovering how genes drive cellular behavior in both healthy and diseased states.
Key Benefits:
- Single-molecule precision: Detect and quantify individual RNA transcripts with high-sensitivity optics and AI-driven analysis.
- Spatial and morphological context: Map gene expression directly to cell structure for deeper biological insight.
- Efficient and reproducible workflows: Automated tiling, stitching, and segmentation ensure complete, reliable transcript counts.
- Proven biological relevance: Captures significant IL-6 upregulation, demonstrating sensitivity to meaningful transcriptional changes.
- Ready for advanced models: Scales from 2D assays to complex 3D systems for drug discovery and disease modeling research.
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Product family
Imaging
Product primary application
IN Carta Image Analysis Software; ImageXpress HCS.ai High-Content Screening System; Laboratory Automation & Customization Solutions; MetaXpress Acquire High-Content Image Acquisition Software; Cancer Research Solutions; Cell Imaging & Analysis; Disease Modeling; Drug Discovery and Development; Toxicology; 3D Cell Models; Cellular Signaling; Cell-Health-Viability-Proliferation-Cytotoxicity-and-Cellular-Function; Nucleic-Acid-Detection-Quantitation-Analysis; Organoid Innovation Center; Organoids; Spheroids; Stem Cell Research
CMP
701Rn00000k8qnBIAQ
Title
Quantitative single-molecule spatial transcriptomics using the ImageXpress HCS.ai High Content Screening System
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